ID   KBTB8_DANRE             Reviewed;         601 AA.
AC   Q08CL3; A8KC38;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Kelch repeat and BTB domain-containing protein 8;
DE   AltName: Full=T-cell activation kelch repeat protein;
DE            Short=TA-KRP;
GN   Name=kbtbd8; Synonyms=takrp; ORFNames=zgc:152950;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=SJD; TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3
CC       ubiquitin ligase complex that acts as a regulator of neural crest
CC       specification (By similarity). The BCR(KBTBD8) complex acts by
CC       mediating monoubiquitination of target proteins (By similarity).
CC       {ECO:0000250|UniProtKB:A0A1B8YAB1, ECO:0000250|UniProtKB:Q8NFY9}.
CC   -!- SUBUNIT: Component of the BCR(KBTBD8) E3 ubiquitin ligase complex.
CC       {ECO:0000250|UniProtKB:Q8NFY9}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC       {ECO:0000250|UniProtKB:Q8NFY9}. Golgi apparatus
CC       {ECO:0000250|UniProtKB:Q8NFY9}. Note=Translocates to the spindle
CC       apparatus during mitosis. {ECO:0000250|UniProtKB:Q8NFY9}.
CC   -!- SIMILARITY: Belongs to the KBTBD8 family. {ECO:0000305}.
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DR   EMBL; BC124188; AAI24189.1; -; mRNA.
DR   EMBL; BC154345; AAI54346.1; -; mRNA.
DR   RefSeq; NP_001103500.1; NM_001110030.1.
DR   AlphaFoldDB; Q08CL3; -.
DR   SMR; Q08CL3; -.
DR   STRING; 7955.ENSDARP00000094847; -.
DR   PaxDb; Q08CL3; -.
DR   GeneID; 559966; -.
DR   KEGG; dre:559966; -.
DR   CTD; 84541; -.
DR   ZFIN; ZDB-GENE-030114-8; kbtbd8.
DR   eggNOG; KOG4441; Eukaryota.
DR   InParanoid; Q08CL3; -.
DR   OrthoDB; 398028at2759; -.
DR   PhylomeDB; Q08CL3; -.
DR   Reactome; R-DRE-8951664; Neddylation.
DR   Reactome; R-DRE-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   PRO; PR:Q08CL3; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR   GO; GO:0014032; P:neural crest cell development; ISS:UniProtKB.
DR   GO; GO:0014029; P:neural crest formation; ISS:UniProtKB.
DR   GO; GO:0006513; P:protein monoubiquitination; ISS:UniProtKB.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 2.120.10.80; -; 1.
DR   Gene3D; 3.30.710.10; -; 1.
DR   InterPro; IPR011705; BACK.
DR   InterPro; IPR017096; BTB-kelch_protein.
DR   InterPro; IPR000210; BTB/POZ_dom.
DR   InterPro; IPR028764; KBTBD8.
DR   InterPro; IPR015915; Kelch-typ_b-propeller.
DR   InterPro; IPR006652; Kelch_1.
DR   InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR   PANTHER; PTHR24412:SF433; PTHR24412:SF433; 1.
DR   Pfam; PF07707; BACK; 1.
DR   Pfam; PF00651; BTB; 1.
DR   Pfam; PF01344; Kelch_1; 2.
DR   PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1.
DR   SMART; SM00875; BACK; 1.
DR   SMART; SM00225; BTB; 1.
DR   SMART; SM00612; Kelch; 4.
DR   SUPFAM; SSF117281; SSF117281; 1.
DR   SUPFAM; SSF54695; SSF54695; 1.
DR   PROSITE; PS50097; BTB; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Cytoskeleton; Golgi apparatus; Kelch repeat; Reference proteome;
KW   Repeat; Translation regulation; Ubl conjugation pathway.
FT   CHAIN           1..601
FT                   /note="Kelch repeat and BTB domain-containing protein 8"
FT                   /id="PRO_0000278222"
FT   DOMAIN          48..116
FT                   /note="BTB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT   DOMAIN          151..253
FT                   /note="BACK"
FT   REPEAT          336..390
FT                   /note="Kelch 1"
FT   REPEAT          391..441
FT                   /note="Kelch 2"
FT   REPEAT          443..481
FT                   /note="Kelch 3"
FT   REPEAT          482..529
FT                   /note="Kelch 4"
FT   REPEAT          542..588
FT                   /note="Kelch 5"
SQ   SEQUENCE   601 AA;  68657 MW;  E9D9DB2DCB7BCFC5 CRC64;
     MAASGDVGKL LQVQNGTPAS TSYNGVDALH ACNILQQLKA LYDEAQLTDI VVEVDHGKTF
     SCHRNVLAAI SPYFRSMFTS GLTESSQREV RIVGVESESM HLVLDYAYTS RVTLTESNVQ
     ALFTAASIFQ IPALQDQCAQ FMISRLDPQN CIGVFMFADA YGHQELRERS QDYIRKKFLC
     VMGEQEFLHL TKDQLVSILN SDDLNVEKEE HVYESIVHWL EYDCSRREAD LPEVFAKCIR
     LPLLEEAFLS RIPPAFALAL SRDSSDKSRL NGTNGCSQRL GMTASEMIIC FDAAHKHSGK
     KQTVPCLDIA AGKVYKLCKP PNDLREVGIL ISSENDIFIA GGYRPSNSEV CIDHRAESDF
     WQYEHAGNRW LPRSPMLRAR IGCRLVHCCG KLYALGGRVY EGDGRNALKS VECYDARDNC
     WTAVSPMPVA MEFHSTIEYK DRIYVLQGEY FFCFDPRKDY WGHLPSMNIP RTQGLAALHK
     NCIYYIAGIC RNHQRTFTVE VYDIEQNTWC RKRDLPFDQA TSPYIKVLLL QGRLHLFVRA
     TQVMVEEHVF RTSRKNSLYQ YDDEADHWTK VYETPDRLWD LGRHFECVVA KLYPQCLQKV
     L