KBTB8_XENTR
ID KBTB8_XENTR Reviewed; 606 AA.
AC A0A1B8YAB1; F6VT55;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2016, sequence version 1.
DT 03-AUG-2022, entry version 26.
DE RecName: Full=Kelch repeat and BTB domain-containing protein 8;
GN Name=kbtbd8;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=20431018; DOI=10.1126/science.1183670;
RA Hellsten U., Harland R.M., Gilchrist M.J., Hendrix D., Jurka J.,
RA Kapitonov V., Ovcharenko I., Putnam N.H., Shu S., Taher L., Blitz I.L.,
RA Blumberg B., Dichmann D.S., Dubchak I., Amaya E., Detter J.C., Fletcher R.,
RA Gerhard D.S., Goodstein D., Graves T., Grigoriev I.V., Grimwood J.,
RA Kawashima T., Lindquist E., Lucas S.M., Mead P.E., Mitros T., Ogino H.,
RA Ohta Y., Poliakov A.V., Pollet N., Robert J., Salamov A., Sater A.K.,
RA Schmutz J., Terry A., Vize P.D., Warren W.C., Wells D., Wills A.,
RA Wilson R.K., Zimmerman L.B., Zorn A.M., Grainger R., Grammer T.,
RA Khokha M.K., Richardson P.M., Rokhsar D.S.;
RT "The genome of the Western clawed frog Xenopus tropicalis.";
RL Science 328:633-636(2010).
RN [2]
RP FUNCTION.
RX PubMed=26399832; DOI=10.1038/nature14978;
RA Werner A., Iwasaki S., McGourty C.A., Medina-Ruiz S., Teerikorpi N.,
RA Fedrigo I., Ingolia N.T., Rape M.;
RT "Cell-fate determination by ubiquitin-dependent regulation of
RT translation.";
RL Nature 525:523-527(2015).
CC -!- FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3
CC ubiquitin ligase complex that acts as a regulator of neural crest
CC specification (PubMed:26399832). The BCR(KBTBD8) complex acts by
CC mediating monoubiquitination of target proteins (By similarity).
CC {ECO:0000250|UniProtKB:Q8NFY9, ECO:0000269|PubMed:26399832}.
CC -!- SUBUNIT: Component of the BCR(KBTBD8) E3 ubiquitin ligase complex.
CC {ECO:0000250|UniProtKB:Q8NFY9}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250|UniProtKB:Q8NFY9}. Golgi apparatus
CC {ECO:0000250|UniProtKB:Q8NFY9}. Note=Translocates to the spindle
CC apparatus during mitosis. {ECO:0000250|UniProtKB:Q8NFY9}.
CC -!- SIMILARITY: Belongs to the KBTBD8 family. {ECO:0000305}.
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DR EMBL; KV460360; OCA19947.1; -; Genomic_DNA.
DR EMBL; AAMC01080009; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAMC01080010; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAMC01080011; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAMC01080012; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; A0A1B8YAB1; -.
DR SMR; A0A1B8YAB1; -.
DR STRING; 8364.ENSXETP00000017686; -.
DR eggNOG; KOG4441; Eukaryota.
DR TreeFam; TF332672; -.
DR Proteomes; UP000008143; Genome assembly.
DR Proteomes; UP000790000; Unplaced.
DR GO; GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0014032; P:neural crest cell development; IMP:UniProtKB.
DR GO; GO:0014029; P:neural crest formation; IMP:UniProtKB.
DR GO; GO:0006513; P:protein monoubiquitination; ISS:UniProtKB.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR Gene3D; 2.120.10.80; -; 1.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR011705; BACK.
DR InterPro; IPR017096; BTB-kelch_protein.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR028764; KBTBD8.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR24412:SF433; PTHR24412:SF433; 1.
DR Pfam; PF07707; BACK; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF01344; Kelch_1; 2.
DR PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1.
DR SMART; SM00875; BACK; 1.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00612; Kelch; 4.
DR SUPFAM; SSF117281; SSF117281; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Cytoskeleton; Golgi apparatus; Kelch repeat; Reference proteome;
KW Repeat; Translation regulation; Ubl conjugation pathway.
FT CHAIN 1..606
FT /note="Kelch repeat and BTB domain-containing protein 8"
FT /id="PRO_0000439643"
FT DOMAIN 55..123
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 158..258
FT /note="BACK"
FT /evidence="ECO:0000255"
FT REPEAT 342..396
FT /note="Kelch 1"
FT /evidence="ECO:0000255"
FT REPEAT 397..447
FT /note="Kelch 2"
FT /evidence="ECO:0000255"
FT REPEAT 449..487
FT /note="Kelch 3"
FT /evidence="ECO:0000255"
FT REPEAT 488..534
FT /note="Kelch 4"
FT /evidence="ECO:0000255"
FT REPEAT 546..593
FT /note="Kelch 5"
FT /evidence="ECO:0000255"
SQ SEQUENCE 606 AA; 69510 MW; 6291F000DB0106EE CRC64;
MAQTPSSTAS TEVAKYVQTQ NGIPPSNPVS SAMDPYHACS ILQQLKAMYD EGKLTDIVVQ
VDHGKHFSCH RNVLAAISPY FRSMFTSGLT ESTQKEVRLV GIEAESMQLV LNYAYTSRVQ
LTEANVQALF TAASIFQIPS LQDQCAQYMI SRLEPQNCIG VFSFADHYGH QELKEKAQDY
IRKKFLYVTK EQEFLHLRKD QLISILNSDD LDVEKEEHVY DSIISWYEHE QETREMHLPE
IFAKCIRMPL MEETFVEQIP PIFAQAMPIN RVQKGKISAN CCTRRLGMTA SEMIICFEAA
NKHSGKKQTV PCLDTLTGKV YKLCKPPGDL REVGILVTPD NELYIAGGYR PSNNDVCIDH
KAESDFWLYD HSSNRWLPKA PLLRARIGCK LVHCCGKLYA IGGRVYEGDG RNPLKSVECY
DTRDNCWTAV SLMPVAMEFH SAVEYKDKIY ILQGEVFLCY DPPRDYWCYL TPMTAPRVQG
MAAVYKDSIY YVAGIRGNHR VLTVEAYDVE QNRWTRKKDL PCEQSSNPYI KLVVFKSKLH
LFVRATQVSV EEFVFRTSRK NSLYQYDEIA DSWKKVYETP ERLWDLGRHF ECAVAKLYPQ
CLQKVL
