ID   KBX2_LIOAU              Reviewed;          80 AA.
AC   C7G3K3;
DT   24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT   13-OCT-2009, sequence version 1.
DT   25-MAY-2022, entry version 16.
DE   RecName: Full=Beta-KTx-like peptide LaIT2 {ECO:0000303|PubMed:19966481};
DE   Flags: Precursor;
OS   Liocheles australasiae (Dwarf wood scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Iurida; Scorpionoidea; Hemiscorpiidae; Liocheles.
OX   NCBI_TaxID=431266;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-63, FUNCTION, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=19966481; DOI=10.1271/bbb.90509;
RA   Matsushita N., Miyashita M., Ichiki Y., Ogura T., Sakuradani E.,
RA   Nakagawa Y., Shimizu S., Miyagawa H.;
RT   "Purification and cDNA cloning of LaIT2, a novel insecticidal toxin from
RT   venom of the scorpion Liocheles australasiae.";
RL   Biosci. Biotechnol. Biochem. 73:2769-2772(2009).
CC   -!- FUNCTION: Amphipathic peptide that causes significant antimicrobial
CC       activity in the growth inhibition assay against E.coli. Causes
CC       paralysis or death to crickets within 48 hours after injection at a
CC       dose of 1.3 ug/insect (26 ug/g of body weight).
CC       {ECO:0000269|PubMed:19966481}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=6628.2; Method=Electrospray; Note=Monoisotopic
CC       mass.; Evidence={ECO:0000269|PubMed:19966481};
CC   -!- SIMILARITY: Belongs to the long chain scorpion toxin family. Class 2
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AB510468; BAI22852.1; -; mRNA.
DR   AlphaFoldDB; C7G3K3; -.
DR   SMR; C7G3K3; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR029237; Long_scorpion_toxin.
DR   Pfam; PF14866; Toxin_38; 1.
DR   PROSITE; PS51862; BSPN_CSAB; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW   Neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000269|PubMed:19966481"
FT   CHAIN           22..80
FT                   /note="Beta-KTx-like peptide LaIT2"
FT                   /id="PRO_5000503610"
FT   DOMAIN          49..80
FT                   /note="BetaSPN-type CS-alpha/beta"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01209"
FT   DISULFID        52..72
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01209"
FT   DISULFID        59..77
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01209"
FT   DISULFID        63..79
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01209"
SQ   SEQUENCE   80 AA;  8984 MW;  450C8BAFD55F6D66 CRC64;
     MAKHLIVMFL VIMVISSLVD CAKKPFVQRV KNAASKAYNK LKGLAMQSQY GCPIISNMCE
     DHCRRKKMEG QCDLLDCVCS