KC1B_BOVIN
ID KC1B_BOVIN Reviewed; 336 AA.
AC P35507; A3KMY0;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Casein kinase I isoform beta;
DE Short=CKI-beta;
DE EC=2.7.11.1;
GN Name=CSNK1B;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=1946367; DOI=10.1073/pnas.88.21.9548;
RA Rowles J., Slaughter C., Moomaw C., Hsu J., Cobb M.H.;
RT "Purification of casein kinase I and isolation of cDNAs encoding multiple
RT casein kinase I-like enzymes.";
RL Proc. Natl. Acad. Sci. U.S.A. 88:9548-9552(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Fetal skin;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Casein kinases are operationally defined by their
CC preferential utilization of acidic proteins such as caseins as
CC substrates. It can phosphorylate a large number of proteins.
CC Participates in Wnt signaling (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- SUBUNIT: Monomer.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. CK1 Ser/Thr
CC protein kinase family. Casein kinase I subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M76544; AAA30452.1; -; mRNA.
DR EMBL; BC133392; AAI33393.1; -; mRNA.
DR PIR; B56406; B56406.
DR RefSeq; NP_001091628.1; NM_001098159.1.
DR RefSeq; XP_010820216.1; XM_010821914.2.
DR RefSeq; XP_010820218.2; XM_010821916.2.
DR AlphaFoldDB; P35507; -.
DR SMR; P35507; -.
DR STRING; 9913.ENSBTAP00000030051; -.
DR PaxDb; P35507; -.
DR Ensembl; ENSBTAT00000030064; ENSBTAP00000030051; ENSBTAG00000039794.
DR GeneID; 785423; -.
DR KEGG; bta:785423; -.
DR CTD; 785423; -.
DR VEuPathDB; HostDB:ENSBTAG00000039794; -.
DR eggNOG; KOG1163; Eukaryota.
DR GeneTree; ENSGT00940000153700; -.
DR HOGENOM; CLU_019279_2_0_1; -.
DR InParanoid; P35507; -.
DR OMA; HEYKVYR; -.
DR OrthoDB; 1097975at2759; -.
DR TreeFam; TF354246; -.
DR BRENDA; 2.7.11.1; 908.
DR Reactome; R-BTA-195253; Degradation of beta-catenin by the destruction complex.
DR Reactome; R-BTA-196299; Beta-catenin phosphorylation cascade.
DR Proteomes; UP000009136; Chromosome X.
DR Bgee; ENSBTAG00000039794; Expressed in uterine horn and 101 other tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IDA:AgBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IDA:AgBase.
DR GO; GO:0090090; P:negative regulation of canonical Wnt signaling pathway; IBA:GO_Central.
DR GO; GO:0018105; P:peptidyl-serine phosphorylation; IDA:AgBase.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cytoplasm; Kinase; Nucleotide-binding; Reference proteome;
KW Serine/threonine-protein kinase; Transferase; Wnt signaling pathway.
FT CHAIN 1..336
FT /note="Casein kinase I isoform beta"
FT /id="PRO_0000192831"
FT DOMAIN 17..285
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 309..336
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 309..327
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 136
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 23..31
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 46
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ SEQUENCE 336 AA; 38754 MW; CE856225C6C7887C CRC64;
MASSSRPKTD VLVGGRYKLV REIGFGSFGH VYLAIDLTNH EQVAVKLESE NTRQPRLLHE
KELYNFLQGG VGIPQIRWYG QETDYNVLVM DLLGPSLEDL FNFCSRRFSM KTVLMLADQM
ISRIEYVHSR NLIHRDIKPD NFLMGTGPQW KKLFLVDFGL AKKYRDNRTG QHIPHRSGKS
FIGTPFCASI SAHLGIEQSR RDDMESIGYV LMYFNRGSLP WQGLKAATLK QKCEKISEMK
MTTPVDVLCK GFPIEFAMYL KYCLRLSFEE APDYRYLRQL FRLLFRKLSY QHDYAFDWIV
LKQKAEQQAS SSSGEGQQAQ TPTGKSDNTK SEMKHS
