ID   KC1_TOXGO               Reviewed;         324 AA.
AC   Q6QNM1;
DT   16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Casein kinase I;
DE            EC=2.7.11.1;
OS   Toxoplasma gondii.
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC   Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma.
OX   NCBI_TaxID=5811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Donald R.G.K., Liberator P.A., Zhong T.;
RT   "Coccidian parasite casein kinase I as a chemotherapeutic target for anti-
RT   protozoal agents.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Casein kinases are operationally defined by their
CC       preferential utilization of acidic proteins such as caseins as
CC       substrates. It can phosphorylate a large number of proteins (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC         [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC         threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC         Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC         EC=2.7.11.1;
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. CK1 Ser/Thr
CC       protein kinase family. Casein kinase I subfamily. {ECO:0000305}.
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DR   EMBL; AY532160; AAS46019.1; -; mRNA.
DR   AlphaFoldDB; Q6QNM1; -.
DR   SMR; Q6QNM1; -.
DR   EnsemblProtists; TGME49_240640-t26_1; TGME49_240640-t26_1; TGME49_240640.
DR   VEuPathDB; ToxoDB:TGARI_240640; -.
DR   VEuPathDB; ToxoDB:TGCAST_240640; -.
DR   VEuPathDB; ToxoDB:TGCOUG_240640; -.
DR   VEuPathDB; ToxoDB:TGDOM2_240640; -.
DR   VEuPathDB; ToxoDB:TGFOU_240640; -.
DR   VEuPathDB; ToxoDB:TGGT1_240640; -.
DR   VEuPathDB; ToxoDB:TGMAS_240640; -.
DR   VEuPathDB; ToxoDB:TGME49_240640; -.
DR   VEuPathDB; ToxoDB:TGP89_240640; -.
DR   VEuPathDB; ToxoDB:TGPRC2_240640; -.
DR   VEuPathDB; ToxoDB:TGRH88_030510; -.
DR   VEuPathDB; ToxoDB:TGRUB_240640; -.
DR   VEuPathDB; ToxoDB:TGVAND_240640; -.
DR   VEuPathDB; ToxoDB:TGVEG_240640; -.
DR   OMA; IFDWTFL; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR   GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR   InterPro; IPR030509; CK1.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   InterPro; IPR017441; Protein_kinase_ATP_BS.
DR   InterPro; IPR008271; Ser/Thr_kinase_AS.
DR   PANTHER; PTHR11909:SF409; PTHR11909:SF409; 1.
DR   Pfam; PF00069; Pkinase; 1.
DR   SMART; SM00220; S_TKc; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR   PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Kinase; Nucleotide-binding; Serine/threonine-protein kinase;
KW   Transferase.
FT   CHAIN           1..324
FT                   /note="Casein kinase I"
FT                   /id="PRO_0000192854"
FT   DOMAIN          9..278
FT                   /note="Protein kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   ACT_SITE        128
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT                   ECO:0000255|PROSITE-ProRule:PRU10027"
FT   BINDING         15..23
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   BINDING         38
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ   SEQUENCE   324 AA;  37960 MW;  06DAA7D90C55E31F CRC64;
     MEVRVGGKYR LGRKIGSGSF GDIYIGANIL TGDEVAIKLE SIKSKHPQLL YESKLYKLLA
     GGIGIPMVHW YGIEGDYNVM VIDLLGPSLE DLFSICNRKL SLKTVLMLAD QMLNRIEFVH
     SKNFIHRDIK PDNFLIGRGK KMSVVYIIDF GLAKKYRDPK TQQHIPYREG KNLTGTARYA
     SINTHLGIEQ SRRDDLEALG YVLMYFNRGS LPWQGLKATT KKDKYDKIME KKMSTPIEIL
     CKHFPFEFIT YLNYCRSLRF EDRPDYAYLR RLFKDLFFRE GYQYDFIFDW TFINTEKDRA
     SRRSQQVYVE DNRQVEENQN ELPM