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KCC2D_PIG
ID   KCC2D_PIG               Reviewed;         499 AA.
AC   Q95266;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 147.
DE   RecName: Full=Calcium/calmodulin-dependent protein kinase type II subunit delta;
DE            Short=CaM kinase II subunit delta;
DE            Short=CaMK-II subunit delta;
DE            EC=2.7.11.17;
GN   Name=CAMK2D;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Aortic smooth muscle;
RX   PubMed=9083077; DOI=10.1074/jbc.272.14.9393;
RA   Singer H.A., Benscoter H.A., Schworer C.M.;
RT   "Novel Ca2+/calmodulin-dependent protein kinase II gamma-subunit variants
RT   expressed in vascular smooth muscle, brain, and cardiomyocytes.";
RL   J. Biol. Chem. 272:9393-9400(1997).
CC   -!- FUNCTION: Calcium/calmodulin-dependent protein kinase involved in the
CC       regulation of Ca(2+) homeostatis and excitation-contraction coupling
CC       (ECC) in heart by targeting ion channels, transporters and accessory
CC       proteins involved in Ca(2+) influx into the myocyte, Ca(2+) release
CC       from the sarcoplasmic reticulum (SR), SR Ca(2+) uptake and Na(+) and
CC       K(+) channel transport. Targets also transcription factors and
CC       signaling molecules to regulate heart function. In its activated form,
CC       is involved in the pathogenesis of dilated cardiomyopathy and heart
CC       failure. Contributes to cardiac decompensation and heart failure by
CC       regulating SR Ca(2+) release via direct phosphorylation of RYR2 Ca(2+)
CC       channel on 'Ser-2808'. In the nucleus, phosphorylates the MEF2
CC       repressor HDAC4, promoting its nuclear export and binding to 14-3-3
CC       protein, and expression of MEF2 and genes involved in the hypertrophic
CC       program. Is essential for left ventricular remodeling responses to
CC       myocardial infarction. In pathological myocardial remodeling acts
CC       downstream of the beta adrenergic receptor signaling cascade to
CC       regulate key proteins involved in ECC. Regulates Ca(2+) influx to
CC       myocytes by binding and phosphorylating the L-type Ca(2+) channel
CC       subunit beta-2 CACNB2. In addition to Ca(2+) channels, can target and
CC       regulate the cardiac sarcolemmal Na(+) channel Nav1.5/SCN5A and the K+
CC       channel Kv4.3/KCND3, which contribute to arrhythmogenesis in heart
CC       failure. Phosphorylates phospholamban (PLN/PLB), an endogenous
CC       inhibitor of SERCA2A/ATP2A2, contributing to the enhancement of SR
CC       Ca(2+) uptake that may be important in frequency-dependent acceleration
CC       of relaxation (FDAR) and maintenance of contractile function during
CC       acidosis. May participate in the modulation of skeletal muscle function
CC       in response to exercise, by regulating SR Ca(2+) transport through
CC       phosphorylation of PLN/PLB and triadin, a ryanodine receptor-coupling
CC       factor (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC         [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC         threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC         Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC         EC=2.7.11.17;
CC   -!- ACTIVITY REGULATION: Activated by Ca(2+)/calmodulin. Binding of
CC       calmodulin results in conformational change that relieves intrasteric
CC       autoinhibition and allows autophosphorylation of Thr-287 which turns
CC       the kinase in a constitutively active form and confers to the kinase a
CC       Ca(2+)-independent activity (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta
CC       (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms
CC       assemble into homo- or heteromultimeric holoenzymes composed of 12
CC       subunits with two hexameric rings stacked one on top of the other.
CC       Interacts with RRAD CACNB2 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane, sarcolemma {ECO:0000305};
CC       Peripheral membrane protein {ECO:0000305}; Cytoplasmic side
CC       {ECO:0000305}. Sarcoplasmic reticulum membrane {ECO:0000305};
CC       Peripheral membrane protein {ECO:0000305}; Cytoplasmic side
CC       {ECO:0000305}.
CC   -!- DOMAIN: The CAMK2 protein kinases contain a unique C-terminal subunit
CC       association domain responsible for oligomerization.
CC   -!- PTM: Autophosphorylation of Thr-287 following activation by
CC       Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an
CC       activated state (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr
CC       protein kinase family. CaMK subfamily. {ECO:0000305}.
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DR   EMBL; U73504; AAC48715.1; -; mRNA.
DR   RefSeq; NP_999546.1; NM_214381.1.
DR   AlphaFoldDB; Q95266; -.
DR   SMR; Q95266; -.
DR   STRING; 9823.ENSSSCP00000009730; -.
DR   PaxDb; Q95266; -.
DR   PeptideAtlas; Q95266; -.
DR   PRIDE; Q95266; -.
DR   Ensembl; ENSSSCT00045024685; ENSSSCP00045017055; ENSSSCG00045012550.
DR   Ensembl; ENSSSCT00055047899; ENSSSCP00055038229; ENSSSCG00055018512.
DR   GeneID; 397674; -.
DR   KEGG; ssc:397674; -.
DR   CTD; 817; -.
DR   eggNOG; KOG0033; Eukaryota.
DR   HOGENOM; CLU_000288_71_0_1; -.
DR   InParanoid; Q95266; -.
DR   OrthoDB; 330091at2759; -.
DR   TreeFam; TF315229; -.
DR   Reactome; R-SSC-3371571; HSF1-dependent transactivation.
DR   Reactome; R-SSC-399719; Trafficking of AMPA receptors.
DR   Reactome; R-SSC-438066; Unblocking of NMDA receptors, glutamate binding and activation.
DR   Reactome; R-SSC-5576892; Phase 0 - rapid depolarisation.
DR   Reactome; R-SSC-5578775; Ion homeostasis.
DR   Reactome; R-SSC-5673001; RAF/MAP kinase cascade.
DR   Reactome; R-SSC-936837; Ion transport by P-type ATPases.
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Unplaced.
DR   Genevisible; Q95266; SS.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR   GO; GO:0042383; C:sarcolemma; IEA:UniProtKB-SubCell.
DR   GO; GO:0033017; C:sarcoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0005516; F:calmodulin binding; IBA:GO_Central.
DR   GO; GO:0004683; F:calmodulin-dependent protein kinase activity; IBA:GO_Central.
DR   GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR   GO; GO:0010613; P:positive regulation of cardiac muscle hypertrophy; ISS:UniProtKB.
DR   GO; GO:0006468; P:protein phosphorylation; ISS:UniProtKB.
DR   GO; GO:0060341; P:regulation of cellular localization; ISS:UniProtKB.
DR   InterPro; IPR013543; Ca/CaM-dep_prot_kinase-assoc.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   InterPro; IPR017441; Protein_kinase_ATP_BS.
DR   InterPro; IPR008271; Ser/Thr_kinase_AS.
DR   Pfam; PF08332; CaMKII_AD; 1.
DR   Pfam; PF00069; Pkinase; 1.
DR   SMART; SM00220; S_TKc; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR   PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; ATP-binding; Calmodulin-binding; Cell membrane; Kinase;
KW   Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome;
KW   Sarcoplasmic reticulum; Serine/threonine-protein kinase; Transferase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q13557"
FT   CHAIN           2..499
FT                   /note="Calcium/calmodulin-dependent protein kinase type II
FT                   subunit delta"
FT                   /id="PRO_0000277818"
FT   DOMAIN          14..272
FT                   /note="Protein kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   REGION          283..292
FT                   /note="Autoinhibitory domain"
FT                   /evidence="ECO:0000250"
FT   REGION          291..301
FT                   /note="Calmodulin-binding"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        136
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT                   ECO:0000255|PROSITE-ProRule:PRU10027"
FT   BINDING         20..28
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   BINDING         43
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:Q13557"
FT   MOD_RES         287
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000250|UniProtKB:Q13557"
FT   MOD_RES         306
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         307
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         315
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q13557"
FT   MOD_RES         318
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PHZ2"
FT   MOD_RES         319
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q13557"
FT   MOD_RES         330
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q13557"
FT   MOD_RES         331
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q13557"
FT   MOD_RES         333
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PHZ2"
FT   MOD_RES         336
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PHZ2"
FT   MOD_RES         337
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q13557"
FT   MOD_RES         404
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q13557"
FT   MOD_RES         490
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q13557"
FT   MOD_RES         494
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PHZ2"
SQ   SEQUENCE   499 AA;  56389 MW;  D6999FB8D41120D3 CRC64;
     MASTTTCTRF TDEYQLFEEL GKGAFSVVRR CMKIPTGQEY AAKIINTKKL SARDHQKLER
     EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI
     LESVNHCHLN GIVHRDLKPE NLLLASKSKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY
     LSPEVLRKDP YGKPVDMWAC GVILYILLVG YPPFWDEDQH RLYQQIKAGA YDFPSPEWDT
     VTPEAKDLIN KMLTINPAKR ITASEALKHP WICQRSTVAS MMHRQETVDC LKKFNARRKL
     KGAILTTMLA TRNFSAAKSL LKKPDGVKES TESSNTTIED EDVKARKQEI IKVTEQLIEA
     INNGDFEAYT KICDPGLTAF EPEALGNLVE GMDFHRFYFE NALSKSNKPI HTIILNPHVH
     LVGDDAACIA YIRLTQYMDG SGMPKTMQSE ETRVWHRRDG KWQNVHFHRS GSPTVPIKPS
     CIPNGKENFS GSTSLWQNI
 
 
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