KCD20_HUMAN
ID KCD20_HUMAN Reviewed; 419 AA.
AC Q7Z5Y7; B4DZD3; B4E2Q3; F5H3T3; Q5W105; Q69YQ7; Q8IZ55;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=BTB/POZ domain-containing protein KCTD20;
DE AltName: Full=Potassium channel tetramerization domain containing 20;
GN Name=KCTD20; Synonyms=C6orf69;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Huang C.Q., Chen Z., Wu S.L., Shan Y.X., Liu S., Xiao P.J.;
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), AND VARIANT
RP THR-171.
RC TISSUE=Trachea;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 34-419 (ISOFORM 1).
RC TISSUE=Skeletal muscle;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [7]
RP INTERACTION WITH MARK4.
RX PubMed=14676191; DOI=10.1074/jbc.m312171200;
RA Brajenovic M., Joberty G., Kuster B., Bouwmeester T., Drewes G.;
RT "Comprehensive proteomic analysis of human Par protein complexes reveals an
RT interconnected protein network.";
RL J. Biol. Chem. 279:12804-12811(2004).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: Promotes the phosphorylation of AKT family members.
CC {ECO:0000250|UniProtKB:Q8CDD8}.
CC -!- SUBUNIT: Interacts with AKT1; AKT2 and AKT3 (By similarity). Associates
CC with PP2CA (By similarity). Part of a complex containing MARK4
CC (PubMed:14676191). {ECO:0000250|UniProtKB:Q8CDD8,
CC ECO:0000269|PubMed:14676191}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8CDD8}.
CC Note=Colocalizes with BTBD10 in filamentous structures.
CC {ECO:0000250|UniProtKB:Q8CDD8}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q7Z5Y7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7Z5Y7-2; Sequence=VSP_021277, VSP_021278;
CC Name=3;
CC IsoId=Q7Z5Y7-3; Sequence=VSP_055669;
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DR EMBL; AY305862; AAP74385.1; -; mRNA.
DR EMBL; AK302861; BAG64045.1; -; mRNA.
DR EMBL; AK304377; BAG65215.1; -; mRNA.
DR EMBL; AL136135; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; Z85986; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471081; EAX03892.1; -; Genomic_DNA.
DR EMBL; BC023525; AAH23525.1; -; mRNA.
DR EMBL; AL832381; CAH10583.1; -; mRNA.
DR CCDS; CCDS4821.1; -. [Q7Z5Y7-1]
DR CCDS; CCDS69096.1; -. [Q7Z5Y7-2]
DR CCDS; CCDS69097.1; -. [Q7Z5Y7-3]
DR RefSeq; NP_001273508.1; NM_001286579.1. [Q7Z5Y7-2]
DR RefSeq; NP_001273509.1; NM_001286580.1. [Q7Z5Y7-3]
DR RefSeq; NP_775833.2; NM_173562.4. [Q7Z5Y7-1]
DR RefSeq; XP_005248997.1; XM_005248940.4. [Q7Z5Y7-1]
DR RefSeq; XP_006715086.1; XM_006715023.2. [Q7Z5Y7-1]
DR RefSeq; XP_011512700.1; XM_011514398.2. [Q7Z5Y7-1]
DR AlphaFoldDB; Q7Z5Y7; -.
DR SMR; Q7Z5Y7; -.
DR BioGRID; 128809; 13.
DR IntAct; Q7Z5Y7; 8.
DR STRING; 9606.ENSP00000362836; -.
DR iPTMnet; Q7Z5Y7; -.
DR PhosphoSitePlus; Q7Z5Y7; -.
DR BioMuta; KCTD20; -.
DR DMDM; 74750149; -.
DR EPD; Q7Z5Y7; -.
DR jPOST; Q7Z5Y7; -.
DR MassIVE; Q7Z5Y7; -.
DR MaxQB; Q7Z5Y7; -.
DR PaxDb; Q7Z5Y7; -.
DR PeptideAtlas; Q7Z5Y7; -.
DR PRIDE; Q7Z5Y7; -.
DR ProteomicsDB; 26368; -.
DR ProteomicsDB; 69360; -. [Q7Z5Y7-1]
DR ProteomicsDB; 69361; -. [Q7Z5Y7-2]
DR Antibodypedia; 29679; 58 antibodies from 17 providers.
DR DNASU; 222658; -.
DR Ensembl; ENST00000373731.7; ENSP00000362836.2; ENSG00000112078.14. [Q7Z5Y7-1]
DR Ensembl; ENST00000449081.6; ENSP00000412205.2; ENSG00000112078.14. [Q7Z5Y7-2]
DR Ensembl; ENST00000536244.5; ENSP00000439118.1; ENSG00000112078.14. [Q7Z5Y7-3]
DR GeneID; 222658; -.
DR KEGG; hsa:222658; -.
DR MANE-Select; ENST00000373731.7; ENSP00000362836.2; NM_173562.5; NP_775833.2.
DR UCSC; uc003ome.4; human. [Q7Z5Y7-1]
DR CTD; 222658; -.
DR DisGeNET; 222658; -.
DR GeneCards; KCTD20; -.
DR HGNC; HGNC:21052; KCTD20.
DR HPA; ENSG00000112078; Low tissue specificity.
DR MIM; 615932; gene.
DR neXtProt; NX_Q7Z5Y7; -.
DR OpenTargets; ENSG00000112078; -.
DR PharmGKB; PA134913479; -.
DR VEuPathDB; HostDB:ENSG00000112078; -.
DR eggNOG; KOG3840; Eukaryota.
DR GeneTree; ENSGT00390000007975; -.
DR HOGENOM; CLU_036245_0_0_1; -.
DR InParanoid; Q7Z5Y7; -.
DR OMA; WNHEPFI; -.
DR OrthoDB; 390051at2759; -.
DR PhylomeDB; Q7Z5Y7; -.
DR TreeFam; TF314369; -.
DR PathwayCommons; Q7Z5Y7; -.
DR SignaLink; Q7Z5Y7; -.
DR BioGRID-ORCS; 222658; 8 hits in 1082 CRISPR screens.
DR ChiTaRS; KCTD20; human.
DR GenomeRNAi; 222658; -.
DR Pharos; Q7Z5Y7; Tdark.
DR PRO; PR:Q7Z5Y7; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q7Z5Y7; protein.
DR Bgee; ENSG00000112078; Expressed in amniotic fluid and 188 other tissues.
DR ExpressionAtlas; Q7Z5Y7; baseline and differential.
DR Genevisible; Q7Z5Y7; HS.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0042327; P:positive regulation of phosphorylation; ISS:UniProtKB.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR039886; BTBD10/KCTD20.
DR InterPro; IPR039885; BTBD10/KCTD20_BTB/POZ.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR21637; PTHR21637; 1.
DR Pfam; PF16017; BTB_3; 1.
DR SMART; SM00225; BTB; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Reference proteome.
FT CHAIN 1..419
FT /note="BTB/POZ domain-containing protein KCTD20"
FT /id="PRO_0000255248"
FT DOMAIN 117..191
FT /note="BTB"
FT VAR_SEQ 1..145
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055669"
FT VAR_SEQ 54
FT /note="D -> G (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_021277"
FT VAR_SEQ 55..220
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_021278"
FT VARIANT 171
FT /note="S -> T (in dbSNP:rs2239808)"
FT /evidence="ECO:0000269|PubMed:14702039"
FT /id="VAR_028854"
FT CONFLICT 70
FT /note="H -> R (in Ref. 6; CAH10583)"
FT /evidence="ECO:0000305"
FT CONFLICT 205
FT /note="L -> P (in Ref. 6; CAH10583)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 419 AA; 47480 MW; 71B5D35EA02714EC CRC64;
MNVHRGSDSD RLLRQEASCL VDDTLAVAQE KEANSLASSG PHNLTYPLGP RNEDLSLDYA
SQPANLQFPH IMPLAEDIKG SCFQSGNKRN HEPFIAPERF GNSSVGFGSN SHSQAPEKVT
LLVDGTRFVV NPQIFTAHPD TMLGRMFGPG REYNFTRPNE KGEYEIAEGI SATVFRTVLD
YYKTGIINCP DGISIPDLRD TCDYLCINFD FNTIRCQDLS ALLHELSNDG AHKQFDHYLE
ELILPIMVGC AKKGERECHI VVLTDEDSVD WDEDHPPPMG EEYSQILYSS KLYRFFKYIE
NRDVAKTVLK ERGLKNIRIG IEGYPTCKEK IKRRPGGRSE VIYNYVQRPF IQMSWEKEEG
KSRHVDFQCV RSKSLTNLVA AGDDVLEDQE ILMHHPPQVD ELDRLNAPLS QMASNDFQD
