KCD20_MOUSE
ID KCD20_MOUSE Reviewed; 419 AA.
AC Q8CDD8; Q3TKH8; Q5U5Z7; Q9CSC4; Q9CWT1;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=BTB/POZ domain-containing protein KCTD20;
DE AltName: Full=Potassium channel tetramerization domain containing 20;
GN Name=Kctd20;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD;
RC TISSUE=Blastocyst, Embryo, Liver, Testis, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 71-419 (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Eye, and Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH AKT1; AKT2;
RP AKT3; PPP1CA AND PP2CA, AND FUNCTION.
RX PubMed=24156551; DOI=10.1186/1471-2091-14-27;
RA Nawa M., Matsuoka M.;
RT "KCTD20, a relative of BTBD10, is a positive regulator of Akt.";
RL BMC Biochem. 14:27-27(2013).
CC -!- FUNCTION: Promotes the phosphorylation of AKT family members.
CC {ECO:0000269|PubMed:24156551}.
CC -!- SUBUNIT: Interacts with AKT1; AKT2 and AKT3 (PubMed:24156551).
CC Interacts with PPP2CA and PPP1CA (PubMed:24156551). Part of a complex
CC containing MARK4 (By similarity). {ECO:0000250|UniProtKB:Q7Z5Y7,
CC ECO:0000269|PubMed:24156551}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:24156551}.
CC Note=Colocalizes with BTBD10 in filamentous structures.
CC {ECO:0000269|PubMed:24156551}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8CDD8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CDD8-2; Sequence=VSP_021279;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC {ECO:0000269|PubMed:24156551}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE39167.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK010404; BAB26913.1; -; mRNA.
DR EMBL; AK013254; BAB28748.2; -; mRNA.
DR EMBL; AK030202; BAC26839.1; -; mRNA.
DR EMBL; AK088807; BAC40584.1; -; mRNA.
DR EMBL; AK166987; BAE39167.1; ALT_INIT; mRNA.
DR EMBL; AK168440; BAE40345.1; -; mRNA.
DR EMBL; BC036318; AAH36318.1; -; mRNA.
DR EMBL; BC049250; AAH49250.1; -; mRNA.
DR CCDS; CCDS28588.1; -. [Q8CDD8-1]
DR CCDS; CCDS89045.1; -. [Q8CDD8-2]
DR RefSeq; NP_080164.2; NM_025888.5. [Q8CDD8-1]
DR RefSeq; XP_006524873.1; XM_006524810.1.
DR RefSeq; XP_006524874.1; XM_006524811.1. [Q8CDD8-1]
DR PDB; 1FO0; X-ray; 2.50 A; P=207-214.
DR PDB; 1NAN; X-ray; 2.30 A; M/Q=207-214.
DR PDB; 2CLZ; X-ray; 1.90 A; C/M=207-214.
DR PDBsum; 1FO0; -.
DR PDBsum; 1NAN; -.
DR PDBsum; 2CLZ; -.
DR AlphaFoldDB; Q8CDD8; -.
DR SMR; Q8CDD8; -.
DR BioGRID; 211857; 6.
DR IntAct; Q8CDD8; 1.
DR MINT; Q8CDD8; -.
DR STRING; 10090.ENSMUSP00000062282; -.
DR iPTMnet; Q8CDD8; -.
DR PhosphoSitePlus; Q8CDD8; -.
DR EPD; Q8CDD8; -.
DR PaxDb; Q8CDD8; -.
DR PRIDE; Q8CDD8; -.
DR ProteomicsDB; 263488; -. [Q8CDD8-1]
DR ProteomicsDB; 263489; -. [Q8CDD8-2]
DR Antibodypedia; 29679; 58 antibodies from 17 providers.
DR DNASU; 66989; -.
DR Ensembl; ENSMUST00000117672; ENSMUSP00000113740; ENSMUSG00000005936. [Q8CDD8-2]
DR Ensembl; ENSMUST00000118762; ENSMUSP00000112890; ENSMUSG00000005936. [Q8CDD8-2]
DR Ensembl; ENSMUST00000168507; ENSMUSP00000131435; ENSMUSG00000005936. [Q8CDD8-1]
DR Ensembl; ENSMUST00000233197; ENSMUSP00000156936; ENSMUSG00000005936. [Q8CDD8-1]
DR GeneID; 66989; -.
DR KEGG; mmu:66989; -.
DR UCSC; uc008brw.1; mouse. [Q8CDD8-1]
DR CTD; 222658; -.
DR MGI; MGI:1914239; Kctd20.
DR VEuPathDB; HostDB:ENSMUSG00000005936; -.
DR eggNOG; KOG3840; Eukaryota.
DR GeneTree; ENSGT00390000007975; -.
DR HOGENOM; CLU_036245_0_0_1; -.
DR InParanoid; Q8CDD8; -.
DR OMA; WNHEPFI; -.
DR OrthoDB; 390051at2759; -.
DR PhylomeDB; Q8CDD8; -.
DR TreeFam; TF314369; -.
DR BioGRID-ORCS; 66989; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Kctd20; mouse.
DR PRO; PR:Q8CDD8; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q8CDD8; protein.
DR Bgee; ENSMUSG00000005936; Expressed in granulocyte and 269 other tissues.
DR ExpressionAtlas; Q8CDD8; baseline and differential.
DR Genevisible; Q8CDD8; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0042327; P:positive regulation of phosphorylation; IDA:UniProtKB.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR039886; BTBD10/KCTD20.
DR InterPro; IPR039885; BTBD10/KCTD20_BTB/POZ.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR21637; PTHR21637; 1.
DR Pfam; PF16017; BTB_3; 1.
DR SMART; SM00225; BTB; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cytoplasm; Reference proteome.
FT CHAIN 1..419
FT /note="BTB/POZ domain-containing protein KCTD20"
FT /id="PRO_0000255249"
FT DOMAIN 117..191
FT /note="BTB"
FT VAR_SEQ 1..71
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_021279"
SQ SEQUENCE 419 AA; 47068 MW; 4354C06990BE5A66 CRC64;
MNVHQGSDGD WSLQPELSCL GDEALAATQE KEGSSLVSSG LHSVTYPLAA RSEDLALDYA
SQPASLPHPH IMPLPEDNKG SCFQSGSKRS HEPFIVPERF GNSGLGFGGG AHSQAPEKVT
LLVDGTRFVV NPQIFTAHPD TMLGRMFGPG REYNFTRPNE KGEYVIAEGI SATVFRTVLD
YYKTGIINCP DGISIPDLRD TCDYLCINFD FNTIRCQDLS ALLHELSNDG AHKQFDHYLE
ELILPIMVGC AKKGERECHI VVLTDEDSVD WDEDHPPPMG EEYSQILYSS KLYRFFKYIE
NRDVAKTVLK ERGLKNIRIG IEGYPTCKEK IKRRPGGRSE VIYNYVQRPF IQMSWEKEEG
KSRHVDFQCV RSKSLTNLVA AGEDVLEDQE IIMHHPPQVD ELDRLNAPLS QMAPNDFQD
