KCJ10_MOUSE
ID KCJ10_MOUSE Reviewed; 379 AA.
AC Q9JM63;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=ATP-sensitive inward rectifier potassium channel 10;
DE AltName: Full=Inward rectifier K(+) channel Kir4.1;
DE AltName: Full=Potassium channel, inwardly rectifying subfamily J member 10;
GN Name=Kcnj10;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Inanobe A., Takahashi K., Tanemoto M., Fujita A., Kurachi Y.;
RL Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=CD-1, and ICR; TISSUE=Brain;
RX PubMed=11169792;
RX DOI=10.1002/1098-1136(20010101)33:1<57::aid-glia1006>3.0.co;2-0;
RA Li L., Head V., Timpe L.C.;
RT "Identification of an inward rectifier potassium channel gene expressed in
RT mouse cortical astrocytes.";
RL Glia 33:57-71(2001).
RN [3]
RP INTERACTION WITH PATJ.
RX PubMed=9647694; DOI=10.1006/mcne.1998.0679;
RA Kurschner C., Mermelstein P.G., Holden W.T., Surmeier D.J.;
RT "CIPP, a novel multivalent PDZ domain protein, selectively interacts with
RT Kir4.0 family members, NMDA receptor subunits, neurexins, and
RT neuroligins.";
RL Mol. Cell. Neurosci. 11:161-172(1998).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=33811157; DOI=10.1681/asn.2020111587;
RA Schlingmann K.P., Renigunta A., Hoorn E.J., Forst A.L., Renigunta V.,
RA Atanasov V., Mahendran S., Barakat T.S., Gillion V., Godefroid N.,
RA Brooks A.S., Lugtenberg D., Lake J., Debaix H., Rudin C., Knebelmann B.,
RA Tellier S., Rousset-Rouviere C., Viering D., de Baaij J.H.F., Weber S.,
RA Palygin O., Staruschenko A., Kleta R., Houillier P., Bockenhauer D.,
RA Devuyst O., Vargas-Poussou R., Warth R., Zdebik A.A., Konrad M.;
RT "Defects in KCNJ16 cause a novel tubulopathy with hypokalemia, salt
RT wasting, disturbed acid-base homeostasis, and sensorineural deafness.";
RL J. Am. Soc. Nephrol. 32:1498-1512(2021).
CC -!- FUNCTION: May be responsible for potassium buffering action of glial
CC cells in the brain. Inward rectifier potassium channels are
CC characterized by a greater tendency to allow potassium to flow into the
CC cell rather than out of it. Their voltage dependence is regulated by
CC the concentration of extracellular potassium; as external potassium is
CC raised, the voltage range of the channel opening shifts to more
CC positive voltages. The inward rectification is mainly due to the
CC blockage of outward current by internal magnesium. Can be blocked by
CC extracellular barium and cesium (By similarity). In the kidney,
CC together with KCNJ16, mediates basolateral K(+) recycling in distal
CC tubules; this process is critical for Na(+) reabsorption at the tubules
CC (By similarity). {ECO:0000250, ECO:0000250|UniProtKB:P78508}.
CC -!- SUBUNIT: Heterodimer with Kir5.1/KCNJ16; this interaction is required
CC for KCNJ16 localization to the basolateral membrane in kidney cells.
CC Interacts with MAGI1, alone and possibly as a heterodimer with KCNJ16;
CC this interaction may facilitate KCNJ10/KCNJ16 potassium channel
CC expression at the basolateral membrane in kidney cells (By similarity).
CC Interacts with PATJ (PubMed:9647694). {ECO:0000250|UniProtKB:P78508,
CC ECO:0000269|PubMed:9647694}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:P78508}; Multi-
CC pass membrane protein {ECO:0000250|UniProtKB:P78508}. Basolateral cell
CC membrane {ECO:0000250|UniProtKB:P78508}. Note=In kidney distal
CC convoluted tubules, located in the basolateral membrane where it
CC colocalizes with KCNJ16. {ECO:0000250|UniProtKB:P78508}.
CC -!- TISSUE SPECIFICITY: Widely expressed in adult brain, including in the
CC neocortex, the stratum pyrimadale of the hippocampus and the piriform
CC cortex. Expressed by cultured astrocytes and also by cocultured
CC cortical neurons (at protein level). Expressed in the distal segment of
CC the nephron (PubMed:33811157). {ECO:0000269|PubMed:11169792,
CC ECO:0000269|PubMed:33811157}.
CC -!- SIMILARITY: Belongs to the inward rectifier-type potassium channel (TC
CC 1.A.2.1) family. KCNJ10 subfamily. {ECO:0000305}.
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DR EMBL; AB039879; BAA92432.1; -; mRNA.
DR EMBL; AF322631; AAG42845.1; -; mRNA.
DR CCDS; CCDS15512.1; -.
DR RefSeq; NP_001034573.1; NM_001039484.1.
DR RefSeq; XP_006496740.2; XM_006496677.3.
DR AlphaFoldDB; Q9JM63; -.
DR SMR; Q9JM63; -.
DR BioGRID; 200895; 11.
DR CORUM; Q9JM63; -.
DR IntAct; Q9JM63; 6.
DR MINT; Q9JM63; -.
DR STRING; 10090.ENSMUSP00000054356; -.
DR iPTMnet; Q9JM63; -.
DR PhosphoSitePlus; Q9JM63; -.
DR SwissPalm; Q9JM63; -.
DR MaxQB; Q9JM63; -.
DR PaxDb; Q9JM63; -.
DR PRIDE; Q9JM63; -.
DR ProteomicsDB; 301767; -.
DR Antibodypedia; 1679; 288 antibodies from 27 providers.
DR DNASU; 16513; -.
DR Ensembl; ENSMUST00000056136; ENSMUSP00000054356; ENSMUSG00000044708.
DR GeneID; 16513; -.
DR KEGG; mmu:16513; -.
DR UCSC; uc007dqj.1; mouse.
DR CTD; 3766; -.
DR MGI; MGI:1194504; Kcnj10.
DR VEuPathDB; HostDB:ENSMUSG00000044708; -.
DR eggNOG; KOG3827; Eukaryota.
DR GeneTree; ENSGT00990000203615; -.
DR HOGENOM; CLU_022738_3_3_1; -.
DR InParanoid; Q9JM63; -.
DR OMA; QVNVAFQ; -.
DR OrthoDB; 956263at2759; -.
DR PhylomeDB; Q9JM63; -.
DR TreeFam; TF313676; -.
DR Reactome; R-MMU-1296041; Activation of G protein gated Potassium channels.
DR Reactome; R-MMU-1296067; Potassium transport channels.
DR Reactome; R-MMU-997272; Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
DR BioGRID-ORCS; 16513; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Kcnj10; mouse.
DR PRO; PR:Q9JM63; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q9JM63; protein.
DR Bgee; ENSMUSG00000044708; Expressed in cerebellar nuclear complex and 108 other tissues.
DR ExpressionAtlas; Q9JM63; baseline and differential.
DR Genevisible; Q9JM63; MM.
DR GO; GO:0016324; C:apical plasma membrane; ISO:MGI.
DR GO; GO:0097449; C:astrocyte projection; IDA:ARUK-UCL.
DR GO; GO:0016323; C:basolateral plasma membrane; IDA:MGI.
DR GO; GO:0044297; C:cell body; IDA:ARUK-UCL.
DR GO; GO:0097546; C:ciliary base; IDA:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; IC:MGI.
DR GO; GO:0005902; C:microvillus; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0098793; C:presynapse; IEA:GOC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0005242; F:inward rectifier potassium channel activity; IMP:MGI.
DR GO; GO:0005267; F:potassium channel activity; IDA:MGI.
DR GO; GO:0005102; F:signaling receptor binding; ISO:MGI.
DR GO; GO:0007628; P:adult walking behavior; IMP:MGI.
DR GO; GO:0035865; P:cellular response to potassium ion; IMP:MGI.
DR GO; GO:0022010; P:central nervous system myelination; IMP:MGI.
DR GO; GO:0051649; P:establishment of localization in cell; IMP:MGI.
DR GO; GO:0051935; P:glutamate reuptake; IMP:MGI.
DR GO; GO:0051938; P:L-glutamate import; ISO:MGI.
DR GO; GO:0060081; P:membrane hyperpolarization; ISO:MGI.
DR GO; GO:1905515; P:non-motile cilium assembly; IMP:MGI.
DR GO; GO:0014003; P:oligodendrocyte development; IMP:MGI.
DR GO; GO:0055075; P:potassium ion homeostasis; IMP:MGI.
DR GO; GO:1990573; P:potassium ion import across plasma membrane; ISO:MGI.
DR GO; GO:0071805; P:potassium ion transmembrane transport; IMP:MGI.
DR GO; GO:0006813; P:potassium ion transport; IDA:MGI.
DR GO; GO:0034765; P:regulation of ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0048169; P:regulation of long-term neuronal synaptic plasticity; IMP:MGI.
DR GO; GO:0042391; P:regulation of membrane potential; IMP:MGI.
DR GO; GO:0060075; P:regulation of resting membrane potential; IMP:MGI.
DR GO; GO:0051930; P:regulation of sensory perception of pain; ISO:MGI.
DR GO; GO:0007601; P:visual perception; IMP:MGI.
DR Gene3D; 2.60.40.1400; -; 1.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR041647; IRK_C.
DR InterPro; IPR016449; K_chnl_inward-rec_Kir.
DR InterPro; IPR003269; K_chnl_inward-rec_Kir1.2.
DR InterPro; IPR013518; K_chnl_inward-rec_Kir_cyto.
DR InterPro; IPR040445; Kir_TM.
DR PANTHER; PTHR11767; PTHR11767; 1.
DR PANTHER; PTHR11767:SF21; PTHR11767:SF21; 1.
DR Pfam; PF01007; IRK; 1.
DR Pfam; PF17655; IRK_C; 1.
DR PIRSF; PIRSF005465; GIRK_kir; 1.
DR PRINTS; PR01322; KIR12CHANNEL.
DR PRINTS; PR01320; KIRCHANNEL.
DR SUPFAM; SSF81296; SSF81296; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell membrane; Ion channel; Ion transport; Membrane;
KW Nucleotide-binding; Potassium; Potassium transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Voltage-gated channel.
FT CHAIN 1..379
FT /note="ATP-sensitive inward rectifier potassium channel 10"
FT /id="PRO_0000154954"
FT TOPO_DOM 1..64
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 65..89
FT /note="Helical; Name=M1"
FT /evidence="ECO:0000250"
FT TOPO_DOM 90..114
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT INTRAMEM 115..126
FT /note="Helical; Pore-forming; Name=H5"
FT /evidence="ECO:0000250"
FT INTRAMEM 127..133
FT /note="Pore-forming"
FT /evidence="ECO:0000250"
FT TOPO_DOM 134..142
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 143..164
FT /note="Helical; Name=M2"
FT /evidence="ECO:0000250"
FT TOPO_DOM 165..379
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT MOTIF 128..133
FT /note="Selectivity filter"
FT /evidence="ECO:0000250"
FT BINDING 210..217
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT SITE 158
FT /note="Role in the control of polyamine-mediated channel
FT gating and in the blocking by intracellular magnesium"
FT /evidence="ECO:0000250"
SQ SEQUENCE 379 AA; 42432 MW; 7FF08446B7F43453 CRC64;
MTSVAKVYYS QTTQTESRPL VAPGIRRRRV LTKDGRSNVR MEHIADKRFL YLKDLWTTFI
DMQWRYKLLL FSATFAGTWF LFGVVWYLVA VAHGDLLELG PPANHTPCVV QVHTLTGAFL
FSLESQTTIG YGFRYISEEC PLAIVLLIAQ LVLTTILEIF ITGTFLAKIA RPKKRAETIR
FSQHAVVASH NGKPCLMIRV ANMRKSLLIG CQVTGKLLQT HQTKEGENIR LNQVNVTFQV
DTASDSPFLI LPLTFYHVVD ETSPLKDLPL RSGEGDFELV LILSGTVEST SATCQVRTSY
LPEEILWGYE FTPAISLSAS GKYIADFSLF DQVVKVASPS GLRDSTVRYG DPEKLKLEES
LREQAEKEGS ALSVRISNV
