KCJ14_RAT
ID KCJ14_RAT Reviewed; 434 AA.
AC O70596;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=ATP-sensitive inward rectifier potassium channel 14;
DE AltName: Full=Inward rectifier K(+) channel Kir2.4;
DE Short=IRK-4;
DE AltName: Full=Potassium channel, inwardly rectifying subfamily J member 14;
GN Name=Kcnj14; Synonyms=Irk4;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, AND TISSUE SPECIFICITY.
RC STRAIN=Sprague-Dawley; TISSUE=Brain;
RX PubMed=9592090; DOI=10.1523/jneurosci.18-11-04096.1998;
RA Toepert C., Doering F., Wischmeyer E., Karschin C., Brockhaus J.,
RA Ballanyi K., Derst C., Karschin A.;
RT "Kir2.4: a novel K+ inward rectifier channel associated with motoneurons of
RT cranial nerve nuclei.";
RL J. Neurosci. 18:4096-4105(1998).
CC -!- FUNCTION: Inward rectifier potassium channels are characterized by a
CC greater tendency to allow potassium to flow into the cell rather than
CC out of it. Their voltage dependence is regulated by the concentration
CC of extracellular potassium; as external potassium is raised, the
CC voltage range of the channel opening shifts to more positive voltages.
CC The inward rectification is mainly due to the blockage of outward
CC current by internal magnesium. KCNJ14 gives rise to low-conductance
CC channels with a low affinity to the channel blockers Barium and Cesium.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed predominantly in motoneurons of cranial
CC nerve motor nuclei within the general somatic and special visceral
CC motor cell column. {ECO:0000269|PubMed:9592090}.
CC -!- SIMILARITY: Belongs to the inward rectifier-type potassium channel (TC
CC 1.A.2.1) family. KCNJ14 subfamily. {ECO:0000305}.
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DR EMBL; AJ003065; CAA05839.1; -; mRNA.
DR RefSeq; NP_733836.1; NM_170718.1.
DR AlphaFoldDB; O70596; -.
DR SMR; O70596; -.
DR STRING; 10116.ENSRNOP00000028591; -.
DR GuidetoPHARMACOLOGY; 433; -.
DR TCDB; 1.A.2.1.5; the inward rectifier k(+) channel (irk-c) family.
DR PaxDb; O70596; -.
DR PRIDE; O70596; -.
DR ABCD; O70596; 1 sequenced antibody.
DR Ensembl; ENSRNOT00000028591; ENSRNOP00000028591; ENSRNOG00000021056.
DR GeneID; 276720; -.
DR KEGG; rno:276720; -.
DR CTD; 3770; -.
DR RGD; 628872; Kcnj14.
DR eggNOG; KOG3827; Eukaryota.
DR GeneTree; ENSGT01030000234586; -.
DR HOGENOM; CLU_022738_3_0_1; -.
DR InParanoid; O70596; -.
DR OMA; CHNGWAP; -.
DR OrthoDB; 956263at2759; -.
DR PhylomeDB; O70596; -.
DR Reactome; R-RNO-1296053; Classical Kir channels.
DR Reactome; R-RNO-5576886; Phase 4 - resting membrane potential.
DR PRO; PR:O70596; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Bgee; ENSRNOG00000021056; Expressed in cerebellum and 19 other tissues.
DR Genevisible; O70596; RN.
DR GO; GO:0030425; C:dendrite; IDA:RGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043025; C:neuronal cell body; IDA:RGD.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005242; F:inward rectifier potassium channel activity; IDA:RGD.
DR GO; GO:1990573; P:potassium ion import across plasma membrane; IBA:GO_Central.
DR GO; GO:0034765; P:regulation of ion transmembrane transport; IBA:GO_Central.
DR Gene3D; 2.60.40.1400; -; 1.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR041647; IRK_C.
DR InterPro; IPR016449; K_chnl_inward-rec_Kir.
DR InterPro; IPR013518; K_chnl_inward-rec_Kir_cyto.
DR InterPro; IPR040445; Kir_TM.
DR PANTHER; PTHR11767; PTHR11767; 1.
DR Pfam; PF01007; IRK; 1.
DR Pfam; PF17655; IRK_C; 1.
DR PIRSF; PIRSF005465; GIRK_kir; 1.
DR PRINTS; PR01320; KIRCHANNEL.
DR SUPFAM; SSF81296; SSF81296; 1.
PE 1: Evidence at protein level;
KW Ion channel; Ion transport; Membrane; Potassium; Potassium transport;
KW Reference proteome; S-nitrosylation; Transmembrane; Transmembrane helix;
KW Transport; Voltage-gated channel.
FT CHAIN 1..434
FT /note="ATP-sensitive inward rectifier potassium channel 14"
FT /id="PRO_0000154970"
FT TOPO_DOM 1..84
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 85..109
FT /note="Helical; Name=M1"
FT /evidence="ECO:0000250"
FT TOPO_DOM 110..131
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT INTRAMEM 132..143
FT /note="Helical; Pore-forming; Name=H5"
FT /evidence="ECO:0000250"
FT INTRAMEM 144..150
FT /note="Pore-forming"
FT /evidence="ECO:0000250"
FT TOPO_DOM 151..159
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 160..181
FT /note="Helical; Name=M2"
FT /evidence="ECO:0000250"
FT TOPO_DOM 182..434
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT REGION 398..434
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 145..150
FT /note="Selectivity filter"
FT /evidence="ECO:0000250"
FT COMPBIAS 413..427
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 79
FT /note="S-nitrosocysteine"
FT /evidence="ECO:0000250|UniProtKB:P63252"
SQ SEQUENCE 434 AA; 47609 MW; AB586F4628AB42EA CRC64;
MGLARALRRL SGALEPGNSR AGDEEEAGAG LCRNGWAPGP VAGNRRRGRF VKKDGHCNVR
FVNLGGQGAR YLSDLFTTCV DVRWRWMCLL FSCSFLASWL LFGLTFWLIA SLHGDLAAPP
PPAPCFSQVA SFLAAFLFAL ETQTSIGYGV RSVTEECPAA VAAVVLQCIA GCVLDAFVVG
AVMAKMAKPK KRNETLVFSE NAVVALRDRR LCLMWRVGNL RRSHLVEAHV RAQLLQPRVT
PEGEYIPLDH QDVDVGFDGG TDRIFLVSPI TIVHEIDSAS PLYELGRAEL ARADFELVVI
LEGMVEATAM TTQCRSSYLP GELLWGHRFE PVLFQRGSQY EVDYRHFHRT YEVPGTPVCS
AKELDERAEQ ASHSPKSSFP GSLAAFCYEN ELALSCCQEE DEEEDTKEGT SAETPDRAAS
PQALTPTLAL TLPP
