KCMB1_HUMAN
ID KCMB1_HUMAN Reviewed; 191 AA.
AC Q16558; O00707; O00708; P78475; Q53YR0; Q8TAX3; Q93005;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 23-MAR-2010, sequence version 5.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=Calcium-activated potassium channel subunit beta-1;
DE AltName: Full=BK channel subunit beta-1;
DE Short=BKbeta;
DE Short=BKbeta1;
DE Short=Hbeta1;
DE AltName: Full=Calcium-activated potassium channel, subfamily M subunit beta-1;
DE Short=Calcium-activated potassium channel subunit beta;
DE AltName: Full=Charybdotoxin receptor subunit beta-1;
DE AltName: Full=K(VCA)beta-1;
DE AltName: Full=Maxi K channel subunit beta-1;
DE AltName: Full=Slo-beta-1;
DE Short=Slo-beta;
GN Name=KCNMB1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Uterus;
RX PubMed=8612769; DOI=10.1016/0014-5793(96)00151-2;
RA Meera P., Wallner M., Jiang Z., Toro L.;
RT "A calcium switch for the functional coupling between alpha (hslo) and beta
RT subunits (KV,Ca beta) of maxi K channels.";
RL FEBS Lett. 382:84-88(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Uterus;
RX PubMed=8764643; DOI=10.1523/jneurosci.16-15-04543.1996;
RA Dworetzky S.I., Boissard C.G., Lum-Ragan J.T., McKay M.C.,
RA Post-Munson D.J., Trojnacki J.T., Chang C.P., Gribkoff V.K.;
RT "Phenotypic alteration of a human BK (hSlo) channel by hSlobeta subunit
RT coexpression: changes in blocker sensitivity, activation/relaxation and
RT inactivation kinetics, and protein kinase A modulation.";
RL J. Neurosci. 16:4543-4550(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=8799178; DOI=10.1073/pnas.93.17.9200;
RA Tseng-Crank J., Godinot N., Johansen T.E., Ahring P.K., Strobaek D.,
RA Mertz R., Foster C.D., Olesen S.P., Reinhart P.H.;
RT "Cloning, expression, and distribution of a Ca(2+)-activated K+ channel
RT beta-subunit from human brain.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:9200-9205(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
RC TISSUE=Aortic smooth muscle;
RA Folander K., Biazzo D., Swanson R.;
RT "Sequence of the gene encoding the beta subunit of a human, large-
RT conductance, calcium-activate, K+ channel.";
RL Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Rae J.L., Shepard A.R.;
RT "Identification of potassium channels in human lens epithelium.";
RL (In) Civan M.M. (eds.);
RL The eye's aqueous humor, from secretion to glaucoma, pp.69-104, Academic
RL Press, San Diego (1998).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Myometrium;
RX PubMed=12434576;
RA Mazzone J.N., Kaiser R.A., Buxton I.L.O.;
RT "Calcium-activated potassium channel expression in human myometrium: effect
RT of pregnancy.";
RL Proc. West. Pharmacol. Soc. 45:184-186(2002).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Pluznick J.L., Sansom S.C.;
RT "Role of the BK-beta1 subunit in human mesangial cells.";
RL Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Cervix;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [10]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [11]
RP GLYCOSYLATION.
RX PubMed=10792058; DOI=10.1073/pnas.100118597;
RA Meera P., Wallner M., Toro L.;
RT "A neuronal beta subunit (KCNMB4) makes the large conductance, voltage- and
RT Ca2+-activated K+ channel resistant to charybdotoxin and iberiotoxin.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:5562-5567(2000).
RN [12]
RP BINDING TO E2.
RX PubMed=10489376; DOI=10.1126/science.285.5435.1929;
RA Valverde M.A., Rojas P., Amigo J., Cosmelli D., Orio P., Bahamonde M.I.,
RA Mann G.E., Vergara C., Latorre R.;
RT "Acute activation of Maxi-K channels (hSlo) by estradiol binding to the
RT beta subunit.";
RL Science 285:1929-1931(1999).
RN [13]
RP REVIEW.
RX PubMed=12136044; DOI=10.1152/nips.01387.2002;
RA Orio P., Rojas P., Ferreira G., Latorre R.;
RT "New disguises for an old channel: MaxiK channel beta-subunits.";
RL News Physiol. Sci. 17:156-161(2002).
RN [14]
RP VARIANT LYS-65, AND POLYMORPHISM.
RX PubMed=15057310; DOI=10.1172/jci200420347;
RA Fernandez-Fernandez J.M., Tomas M., Vazquez E., Orio P., Latorre R.,
RA Senti M., Marrugat J., Valverde M.A.;
RT "Gain-of-function mutation in the KCNMB1 potassium channel subunit is
RT associated with low prevalence of diastolic hypertension.";
RL J. Clin. Invest. 113:1032-1039(2004).
CC -!- FUNCTION: Regulatory subunit of the calcium activated potassium KCNMA1
CC (maxiK) channel. Modulates the calcium sensitivity and gating kinetics
CC of KCNMA1, thereby contributing to KCNMA1 channel diversity. Increases
CC the apparent Ca(2+)/voltage sensitivity of the KCNMA1 channel. It also
CC modifies KCNMA1 channel kinetics and alters its pharmacological
CC properties. It slows down the activation and the deactivation kinetics
CC of the channel. Acts as a negative regulator of smooth muscle
CC contraction by enhancing the calcium sensitivity to KCNMA1. Its
CC presence is also a requirement for internal binding of the KCNMA1
CC channel opener dehydrosoyasaponin I (DHS-1) triterpene glycoside and
CC for external binding of the agonist hormone 17-beta-estradiol (E2).
CC Increases the binding activity of charybdotoxin (CTX) toxin to KCNMA1
CC peptide blocker by increasing the CTX association rate and decreasing
CC the dissociation rate.
CC -!- SUBUNIT: Interacts with KCNMA1 tetramer. There are probably 4 molecules
CC of KCMNB1 per KCNMA1 tetramer.
CC -!- INTERACTION:
CC Q16558-2; Q9UNK0: STX8; NbExp=3; IntAct=EBI-17703887, EBI-727240;
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q16558-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q16558-2; Sequence=VSP_009822, VSP_009823;
CC -!- TISSUE SPECIFICITY: Abundantly expressed in smooth muscle. Low levels
CC of expression in most other tissues. Within the brain, relatively high
CC levels found in hippocampus and corpus callosum.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:10792058}.
CC -!- POLYMORPHISM: Genetic variation in KCNMB1 can influence the severity of
CC diastolic hypertension (PubMed:15057310).
CC {ECO:0000269|PubMed:15057310}.
CC -!- SIMILARITY: Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB1
CC subfamily. {ECO:0000305}.
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DR EMBL; U25138; AAB02394.1; -; mRNA.
DR EMBL; U38907; AAA81327.1; -; mRNA.
DR EMBL; U42600; AAB16827.1; -; mRNA.
DR EMBL; U42601; AAB16825.1; -; Genomic_DNA.
DR EMBL; U42603; AAB16826.1; -; Genomic_DNA.
DR EMBL; U42602; AAB16826.1; JOINED; Genomic_DNA.
DR EMBL; U61536; AAB61396.1; -; mRNA.
DR EMBL; U61537; AAB61397.1; -; Genomic_DNA.
DR EMBL; AF026002; AAB88805.1; -; mRNA.
DR EMBL; AY044441; AAK95827.1; -; mRNA.
DR EMBL; AY515264; AAS20193.1; -; mRNA.
DR EMBL; AK313979; BAG36693.1; -; mRNA.
DR EMBL; CH471062; EAW61474.1; -; Genomic_DNA.
DR EMBL; BC025707; AAH25707.1; -; mRNA.
DR CCDS; CCDS4373.1; -. [Q16558-1]
DR PIR; S68842; S68842.
DR RefSeq; NP_004128.1; NM_004137.3. [Q16558-1]
DR AlphaFoldDB; Q16558; -.
DR SMR; Q16558; -.
DR BioGRID; 109980; 7.
DR IntAct; Q16558; 2.
DR STRING; 9606.ENSP00000274629; -.
DR BindingDB; Q16558; -.
DR ChEMBL; CHEMBL5078; -.
DR DrugBank; DB01110; Miconazole.
DR DrugBank; DB00721; Procaine.
DR DrugBank; DB09089; Trimebutine.
DR TCDB; 8.A.14.1.2; the ca(2+)-activated k(+) channel auxiliary subunit slowpoke-Beta (sloBeta) family.
DR GlyGen; Q16558; 2 sites.
DR PhosphoSitePlus; Q16558; -.
DR BioMuta; KCNMB1; -.
DR DMDM; 292495100; -.
DR MassIVE; Q16558; -.
DR PaxDb; Q16558; -.
DR PeptideAtlas; Q16558; -.
DR PRIDE; Q16558; -.
DR ProteomicsDB; 60914; -. [Q16558-1]
DR ProteomicsDB; 60915; -. [Q16558-2]
DR Antibodypedia; 16897; 190 antibodies from 27 providers.
DR DNASU; 3779; -.
DR Ensembl; ENST00000274629.9; ENSP00000274629.3; ENSG00000145936.9. [Q16558-1]
DR Ensembl; ENST00000521859.1; ENSP00000427940.1; ENSG00000145936.9. [Q16558-2]
DR GeneID; 3779; -.
DR KEGG; hsa:3779; -.
DR MANE-Select; ENST00000274629.9; ENSP00000274629.3; NM_004137.4; NP_004128.1.
DR UCSC; uc003maq.3; human. [Q16558-1]
DR CTD; 3779; -.
DR DisGeNET; 3779; -.
DR GeneCards; KCNMB1; -.
DR HGNC; HGNC:6285; KCNMB1.
DR HPA; ENSG00000145936; Tissue enhanced (endometrium, intestine, seminal vesicle, smooth muscle).
DR MalaCards; KCNMB1; -.
DR MIM; 603951; gene.
DR MIM; 608622; phenotype.
DR neXtProt; NX_Q16558; -.
DR OpenTargets; ENSG00000145936; -.
DR PharmGKB; PA221; -.
DR VEuPathDB; HostDB:ENSG00000145936; -.
DR eggNOG; ENOG502RZA0; Eukaryota.
DR GeneTree; ENSGT00950000183039; -.
DR HOGENOM; CLU_085739_1_1_1; -.
DR InParanoid; Q16558; -.
DR OMA; FPYPCLQ; -.
DR OrthoDB; 1178937at2759; -.
DR PhylomeDB; Q16558; -.
DR TreeFam; TF328589; -.
DR PathwayCommons; Q16558; -.
DR Reactome; R-HSA-1296052; Ca2+ activated K+ channels.
DR Reactome; R-HSA-418457; cGMP effects.
DR Reactome; R-HSA-9662360; Sensory processing of sound by inner hair cells of the cochlea.
DR Reactome; R-HSA-9667769; Acetylcholine inhibits contraction of outer hair cells.
DR SignaLink; Q16558; -.
DR BioGRID-ORCS; 3779; 11 hits in 1068 CRISPR screens.
DR ChiTaRS; KCNMB1; human.
DR GeneWiki; KCNMB1; -.
DR GenomeRNAi; 3779; -.
DR Pharos; Q16558; Tbio.
DR PRO; PR:Q16558; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q16558; protein.
DR Bgee; ENSG00000145936; Expressed in blood vessel layer and 123 other tissues.
DR Genevisible; Q16558; HS.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0008076; C:voltage-gated potassium channel complex; IBA:GO_Central.
DR GO; GO:0015269; F:calcium-activated potassium channel activity; IBA:GO_Central.
DR GO; GO:0015459; F:potassium channel regulator activity; IBA:GO_Central.
DR GO; GO:0007568; P:aging; IEA:Ensembl.
DR GO; GO:1903413; P:cellular response to bile acid; IEA:Ensembl.
DR GO; GO:0071361; P:cellular response to ethanol; IEA:Ensembl.
DR GO; GO:0071456; P:cellular response to hypoxia; IEA:Ensembl.
DR GO; GO:0007268; P:chemical synaptic transmission; TAS:ProtInc.
DR GO; GO:0005513; P:detection of calcium ion; IBA:GO_Central.
DR GO; GO:1901381; P:positive regulation of potassium ion transmembrane transport; IEA:Ensembl.
DR GO; GO:0006813; P:potassium ion transport; TAS:ProtInc.
DR GO; GO:0042311; P:vasodilation; IEA:Ensembl.
DR InterPro; IPR003930; K_chnl_Ca-activ_BK_bsu.
DR PANTHER; PTHR10258; PTHR10258; 1.
DR Pfam; PF03185; CaKB; 1.
DR PRINTS; PR01450; BKCHANNELB.
PE 1: Evidence at protein level;
KW Alternative splicing; Glycoprotein; Ion channel; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..191
FT /note="Calcium-activated potassium channel subunit beta-1"
FT /id="PRO_0000187046"
FT TOPO_DOM 1..18
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 19..39
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 40..157
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 179..191
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 80
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 142
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 103..130
FT /note="CSYIPGSVDNYQTARADVEKVRAKFQEQ -> VLNWRDGDTSLYPCQVCEPV
FT PNCPCPRG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_009822"
FT VAR_SEQ 131..191
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_009823"
FT VARIANT 65
FT /note="E -> K (has a protective effect against diastolic
FT hypertension; dbSNP:rs11739136)"
FT /evidence="ECO:0000269|PubMed:15057310"
FT /id="VAR_019325"
FT VARIANT 110
FT /note="V -> L (in dbSNP:rs2301149)"
FT /id="VAR_047009"
SQ SEQUENCE 191 AA; 21797 MW; 547432FF194E770D CRC64;
MVKKLVMAQK RGETRALCLG VTMVVCAVIT YYILVTTVLP LYQKSVWTQE SKCHLIETNI
RDQEELKGKK VPQYPCLWVN VSAAGRWAVL YHTEDTRDQN QQCSYIPGSV DNYQTARADV
EKVRAKFQEQ QVFYCFSAPR GNETSVLFQR LYGPQALLFS LFWPTFLLTG GLLIIAMVKS
NQYLSILAAQ K
