APX1_HYDVD
ID APX1_HYDVD Reviewed; 367 AA.
AC Q539E5;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 53.
DE RecName: Full=Putative ascorbate peroxidase;
DE EC=1.11.1.11;
DE AltName: Full=HvAPX1;
DE Flags: Precursor;
GN Name=APX1;
OS Hydra viridissima (Green hydra) (Chlorohydra viridissima).
OC Eukaryota; Metazoa; Cnidaria; Hydrozoa; Hydroidolina; Anthoathecata;
OC Aplanulata; Hydridae; Hydra.
OX NCBI_TaxID=6082;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=A99;
RX PubMed=15914659; DOI=10.1242/jeb.01571;
RA Habetha M., Bosch T.C.G.;
RT "Symbiotic Hydra express a plant-like peroxidase gene during oogenesis.";
RL J. Exp. Biol. 208:2157-2165(2005).
CC -!- FUNCTION: May play a role in the protection of oocyte incorporated
CC cells from rapid apoptotic degradation.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O2 + L-ascorbate = 2 H2O + L-dehydroascorbate;
CC Xref=Rhea:RHEA:22996, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240,
CC ChEBI:CHEBI:38290, ChEBI:CHEBI:58539; EC=1.11.1.11;
CC -!- COFACTOR:
CC Name=heme b; Xref=ChEBI:CHEBI:60344;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00297};
CC Note=Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.
CC {ECO:0000255|PROSITE-ProRule:PRU00297};
CC -!- DEVELOPMENTAL STAGE: Expressed exclusively during oogenesis.
CC {ECO:0000269|PubMed:15914659}.
CC -!- MISCELLANEOUS: The gene for this protein may have been transferred
CC horizontally following an endosymbiotic event early in evolution of the
CC Hydra lineage as an RNA or cDNA intermediate.
CC -!- SIMILARITY: Belongs to the peroxidase family. {ECO:0000305}.
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DR EMBL; AY608909; AAU07981.1; -; mRNA.
DR AlphaFoldDB; Q539E5; -.
DR SMR; Q539E5; -.
DR PeroxiBase; 2285; HviNAnPrx01.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0016688; F:L-ascorbate peroxidase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0034599; P:cellular response to oxidative stress; IEA:InterPro.
DR GO; GO:0042744; P:hydrogen peroxide catabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR044831; Ccp1-like.
DR InterPro; IPR002016; Haem_peroxidase.
DR InterPro; IPR010255; Haem_peroxidase_sf.
DR InterPro; IPR019793; Peroxidases_heam-ligand_BS.
DR PANTHER; PTHR31356; PTHR31356; 1.
DR Pfam; PF00141; peroxidase; 1.
DR PRINTS; PR00458; PEROXIDASE.
DR SUPFAM; SSF48113; SSF48113; 1.
DR PROSITE; PS00435; PEROXIDASE_1; 1.
DR PROSITE; PS50873; PEROXIDASE_4; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Heme; Hydrogen peroxide; Iron; Metal-binding;
KW Oxidoreductase; Peroxidase; Signal.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT CHAIN 17..367
FT /note="Putative ascorbate peroxidase"
FT /id="PRO_0000023637"
FT ACT_SITE 58
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00297"
FT SITE 54
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00297"
FT CARBOHYD 119
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 239
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 356
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 60..66
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00297"
SQ SEQUENCE 367 AA; 40790 MW; 91E0A494F416A10D CRC64;
MVPVIWLTVL IVLVDSLQII PTFHDFQRAK TDLLGLIESV KRGDDLPMIA GTVRLAFHDC
IGKGKCDGCI DHSKPGNAGL KRVTDRLDAL YDASYKGKIS RADFYALASV TALTRSTANL
SDKYNGLRKF KVGRKDCSTS PVESIDSSDI PRGSDGTSKT LQFFKSEFGM KTQEAVALLG
AHTLGRCSLQ NSGFVGSWVD QRFSTAPPGE ENLSPTSILD NAYYRMIIDI VPWTQVNING
TRIQWQEPSN SIPNDKLPES KRSPLLLNSD MAISWIIKPS DALGTVSCRP TSLKTPCRHS
NAHTFAKIYA KNNALWVKDF TKAFNKMIEM NENKLRKAPI FNGYFDEMNS HDEPINESVD
EISEDIF