KCNE2_RAT
ID KCNE2_RAT Reviewed; 123 AA.
AC P63161; Q5FVT9; Q9WTW0;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Potassium voltage-gated channel subfamily E member 2;
DE AltName: Full=MinK-related peptide 1;
DE AltName: Full=Minimum potassium ion channel-related peptide 1;
DE AltName: Full=Potassium channel subunit beta MiRP1;
GN Name=Kcne2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Heart;
RX PubMed=10219239; DOI=10.1016/s0092-8674(00)80728-x;
RA Abbott G.W., Sesti F., Splawski I., Buck M.E., Lehmann M.H., Timothy K.W.,
RA Keating M.T., Goldstein S.A.N.;
RT "MiRP1 forms IKr potassium channels with HERG and is associated with
RT cardiac arrhythmia.";
RL Cell 97:175-187(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH HCN1.
RX PubMed=11420311; DOI=10.1161/hh1201.093511;
RA Yu H., Wu J., Potapova I., Wymore R.T., Holmes B., Zuckerman J., Pan Z.,
RA Wang H., Shi W., Robinson R.B., El-Maghrabi M.R., Benjamin W., Dixon J.E.,
RA McKinnon D., Cohen I.S., Wymore R.;
RT "MinK-related peptide 1: a beta subunit for the HCN ion channel subunit
RT family enhances expression and speeds activation.";
RL Circ. Res. 88:E84-E87(2001).
RN [4]
RP SUBUNIT, INTERACTION WITH KCNC2, AND SUBCELLULAR LOCATION.
RX PubMed=14679187; DOI=10.1074/jbc.m310501200;
RA Lewis A., McCrossan Z.A., Abbott G.W.;
RT "MinK, MiRP1, and MiRP2 diversify Kv3.1 and Kv3.2 potassium channel
RT gating.";
RL J. Biol. Chem. 279:7884-7892(2004).
RN [5]
RP FUNCTION, INTERACTION WITH KCNB1, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=19219384; DOI=10.1007/s00232-009-9154-8;
RA McCrossan Z.A., Roepke T.K., Lewis A., Panaghie G., Abbott G.W.;
RT "Regulation of the Kv2.1 potassium channel by MinK and MiRP1.";
RL J. Membr. Biol. 228:1-14(2009).
CC -!- FUNCTION: Ancillary protein that assembles as a beta subunit with a
CC voltage-gated potassium channel complex of pore-forming alpha subunits.
CC Modulates the gating kinetics and enhances stability of the channel
CC complex. Assembled with KCNB1 modulates the gating characteristics of
CC the delayed rectifier voltage-dependent potassium channel KCNB1
CC (PubMed:19219384). Associated with KCNH2/HERG is proposed to form the
CC rapidly activating component of the delayed rectifying potassium
CC current in heart (IKr). May associate with KCNQ2 and/or KCNQ3 and
CC modulate the native M-type current. May associate with HCN1 and HCN2
CC and increase potassium current (By similarity). Interacts with KCNQ1;
CC forms a heterooligomer complex leading to currents with an apparently
CC instantaneous activation, a rapid deactivation process and a linear
CC current-voltage relationship and decreases the amplitude of the outward
CC current (By similarity). {ECO:0000250|UniProtKB:Q9Y6J6,
CC ECO:0000269|PubMed:19219384}.
CC -!- SUBUNIT: Interacts with KCNB1 (PubMed:19219384). Associates with
CC KCNH2/ERG1 (By similarity). May associate with KCNQ2 and KCNQ3 (By
CC similarity). Associates with HCN1 and probably HCN2 (PubMed:11420311).
CC Heteromultimer with KCNC2. Interacts with KCNC2 (PubMed:14679187).
CC Interacts with KCNQ1; forms a heterooligomer complex that targets to
CC the membrane raft and leading to currents with an apparently
CC instantaneous activation, a rapid deactivation process and a linear
CC current-voltage relationship and decreases the amplitude of the outward
CC current (By similarity). {ECO:0000250|UniProtKB:Q9Y6J6,
CC ECO:0000269|PubMed:11420311, ECO:0000269|PubMed:14679187,
CC ECO:0000269|PubMed:19219384}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:14679187,
CC ECO:0000269|PubMed:19219384}; Single-pass type I membrane protein
CC {ECO:0000305}. Note=Colocalizes with KCNB1 at the plasma membrane.
CC {ECO:0000269|PubMed:19219384}.
CC -!- TISSUE SPECIFICITY: Expressed in the heart (PubMed:19219384).
CC {ECO:0000269|PubMed:19219384}.
CC -!- SIMILARITY: Belongs to the potassium channel KCNE family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF071003; AAD28087.1; -; mRNA.
DR EMBL; BC089778; AAH89778.1; -; mRNA.
DR RefSeq; NP_598287.1; NM_133603.2.
DR RefSeq; XP_006248085.1; XM_006248023.3.
DR RefSeq; XP_008766776.1; XM_008768554.2.
DR AlphaFoldDB; P63161; -.
DR SMR; P63161; -.
DR CORUM; P63161; -.
DR STRING; 10116.ENSRNOP00000045279; -.
DR GlyGen; P63161; 2 sites.
DR iPTMnet; P63161; -.
DR PhosphoSitePlus; P63161; -.
DR PaxDb; P63161; -.
DR Ensembl; ENSRNOT00000040844; ENSRNOP00000045279; ENSRNOG00000029811.
DR Ensembl; ENSRNOT00000093948; ENSRNOP00000081079; ENSRNOG00000029811.
DR Ensembl; ENSRNOT00000094999; ENSRNOP00000078157; ENSRNOG00000029811.
DR Ensembl; ENSRNOT00000099713; ENSRNOP00000080461; ENSRNOG00000029811.
DR GeneID; 171138; -.
DR KEGG; rno:171138; -.
DR UCSC; RGD:621383; rat.
DR CTD; 9992; -.
DR RGD; 621383; Kcne2.
DR eggNOG; ENOG502S1GJ; Eukaryota.
DR GeneTree; ENSGT00940000154497; -.
DR HOGENOM; CLU_1991831_0_0_1; -.
DR InParanoid; P63161; -.
DR OMA; DYVILYL; -.
DR OrthoDB; 1493495at2759; -.
DR PhylomeDB; P63161; -.
DR TreeFam; TF336058; -.
DR Reactome; R-RNO-5576890; Phase 3 - rapid repolarisation.
DR Reactome; R-RNO-5576893; Phase 2 - plateau phase.
DR PRO; PR:P63161; -.
DR Proteomes; UP000002494; Chromosome 11.
DR Bgee; ENSRNOG00000029811; Expressed in ovary and 17 other tissues.
DR Genevisible; P63161; RN.
DR GO; GO:0009986; C:cell surface; IDA:RGD.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:RGD.
DR GO; GO:0005794; C:Golgi apparatus; IDA:RGD.
DR GO; GO:0016021; C:integral component of membrane; IDA:RGD.
DR GO; GO:0016020; C:membrane; ISO:RGD.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0008076; C:voltage-gated potassium channel complex; IDA:BHF-UCL.
DR GO; GO:0005261; F:cation channel activity; TAS:RGD.
DR GO; GO:0005251; F:delayed rectifier potassium channel activity; IEA:Ensembl.
DR GO; GO:0042802; F:identical protein binding; IPI:RGD.
DR GO; GO:0005242; F:inward rectifier potassium channel activity; IEA:Ensembl.
DR GO; GO:0015459; F:potassium channel regulator activity; IDA:UniProtKB.
DR GO; GO:0044325; F:transmembrane transporter binding; IDA:BHF-UCL.
DR GO; GO:0005249; F:voltage-gated potassium channel activity; IDA:RGD.
DR GO; GO:1902282; F:voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization; ISO:RGD.
DR GO; GO:0007568; P:aging; IEP:RGD.
DR GO; GO:0086002; P:cardiac muscle cell action potential involved in contraction; ISO:RGD.
DR GO; GO:0071466; P:cellular response to xenobiotic stimulus; IDA:BHF-UCL.
DR GO; GO:0015705; P:iodide transport; ISO:RGD.
DR GO; GO:0086009; P:membrane repolarization; IDA:BHF-UCL.
DR GO; GO:0086011; P:membrane repolarization during action potential; IDA:BHF-UCL.
DR GO; GO:0098915; P:membrane repolarization during ventricular cardiac muscle cell action potential; ISO:RGD.
DR GO; GO:0033555; P:multicellular organismal response to stress; ISO:RGD.
DR GO; GO:0015798; P:myo-inositol transport; ISO:RGD.
DR GO; GO:1902260; P:negative regulation of delayed rectifier potassium channel activity; IDA:UniProtKB.
DR GO; GO:1903817; P:negative regulation of voltage-gated potassium channel activity; IDA:RGD.
DR GO; GO:1901800; P:positive regulation of proteasomal protein catabolic process; ISO:RGD.
DR GO; GO:1901387; P:positive regulation of voltage-gated calcium channel activity; IDA:RGD.
DR GO; GO:0097623; P:potassium ion export across plasma membrane; IDA:BHF-UCL.
DR GO; GO:1990573; P:potassium ion import across plasma membrane; IMP:BHF-UCL.
DR GO; GO:0071805; P:potassium ion transmembrane transport; IDA:BHF-UCL.
DR GO; GO:1902159; P:regulation of cyclic nucleotide-gated ion channel activity; IDA:RGD.
DR GO; GO:1902259; P:regulation of delayed rectifier potassium channel activity; IDA:BHF-UCL.
DR GO; GO:0086091; P:regulation of heart rate by cardiac conduction; ISO:RGD.
DR GO; GO:1901979; P:regulation of inward rectifier potassium channel activity; IDA:BHF-UCL.
DR GO; GO:0060306; P:regulation of membrane repolarization; IDA:BHF-UCL.
DR GO; GO:1901379; P:regulation of potassium ion transmembrane transport; IDA:BHF-UCL.
DR GO; GO:0060307; P:regulation of ventricular cardiac muscle cell membrane repolarization; ISO:RGD.
DR GO; GO:0043586; P:tongue development; IEP:RGD.
DR GO; GO:0086005; P:ventricular cardiac muscle cell action potential; ISO:RGD.
DR InterPro; IPR000369; K_chnl_KCNE.
DR InterPro; IPR005425; K_chnl_volt-dep_bsu_KCNE2.
DR PANTHER; PTHR15282; PTHR15282; 1.
DR Pfam; PF02060; ISK_Channel; 1.
DR PRINTS; PR01605; KCNE2CHANNEL.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Ion channel; Ion transport; Membrane;
KW Potassium; Potassium channel; Potassium transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Voltage-gated channel.
FT CHAIN 1..123
FT /note="Potassium voltage-gated channel subfamily E member
FT 2"
FT /id="PRO_0000144288"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 70..123
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 6
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 29
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 123 AA; 14356 MW; CB91870E7B1EB82A CRC64;
MTTLANLTQT LEDAFKKVFI TYMDSWRRNT TAEQQALQAR VDAENFYYVI LYLMVMIGMF
AFIVVAILVS TVKSKRREHS QDPYHQYIVE DWQQKYRSQI LHLEDSKATI HENLGATGFT
VSP
