KCNE3_MOUSE
ID KCNE3_MOUSE Reviewed; 103 AA.
AC Q9WTW2;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Potassium voltage-gated channel subfamily E member 3;
DE AltName: Full=MinK-related peptide 2;
DE AltName: Full=Minimum potassium ion channel-related peptide 2;
DE AltName: Full=Potassium channel subunit beta MiRP2;
GN Name=Kcne3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Abbott G.W., Sesti F., Buck M.E., Goldstein S.A.N.;
RL Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Ancillary protein that assembles as a beta subunit with a
CC voltage-gated potassium channel complex of pore-forming alpha subunits.
CC Modulates the gating kinetics and enhances stability of the channel
CC complex. Assembled with KCNB1 modulates the gating characteristics of
CC the delayed rectifier voltage-dependent potassium channel KCNB1.
CC Associated with KCNC4/Kv3.4 is proposed to form the subthreshold
CC voltage-gated potassium channel in skeletal muscle and to establish the
CC resting membrane potential (RMP) in muscle cells. Associated with
CC KCNQ1/KCLQT1 may form the intestinal cAMP-stimulated potassium channel
CC involved in chloride secretion that produces a current with nearly
CC instantaneous activation with a linear current-voltage relationship.
CC {ECO:0000250|UniProtKB:Q9JJV7, ECO:0000250|UniProtKB:Q9Y6H6}.
CC -!- SUBUNIT: Interacts with KCNB1. Interacts with KCNC2 (By similarity).
CC Associates with KCNC4/Kv3.4 (By similarity). Interacts with KCNQ1;
CC produces a current with nearly instantaneous activation with a linear
CC current-voltage relationship and alters membrane raft localization (By
CC similarity). {ECO:0000250|UniProtKB:Q9JJV7,
CC ECO:0000250|UniProtKB:Q9Y6H6}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9JJV7,
CC ECO:0000250|UniProtKB:Q9Y6H6}; Single-pass type I membrane protein
CC {ECO:0000250|UniProtKB:Q9Y6H6}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q9JJV7, ECO:0000250|UniProtKB:Q9Y6H6}.
CC Perikaryon {ECO:0000250|UniProtKB:Q9Y6H6}. Cell projection, dendrite
CC {ECO:0000250|UniProtKB:Q9Y6H6}. Membrane raft
CC {ECO:0000250|UniProtKB:Q9Y6H6}. Note=Colocalizes with KCNB1 at high-
CC density somatodendritic clusters on the surface of hippocampal neurons.
CC {ECO:0000250|UniProtKB:Q9Y6H6}.
CC -!- SIMILARITY: Belongs to the potassium channel KCNE family.
CC {ECO:0000305}.
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DR EMBL; AF076532; AAD28090.1; -; mRNA.
DR EMBL; BC004629; AAH04629.1; -; mRNA.
DR CCDS; CCDS21495.1; -.
DR RefSeq; NP_001177798.1; NM_001190869.1.
DR RefSeq; NP_001177799.1; NM_001190870.1.
DR RefSeq; NP_001177800.1; NM_001190871.2.
DR RefSeq; NP_001177879.1; NM_001190950.1.
DR RefSeq; NP_065599.1; NM_020574.5.
DR RefSeq; XP_006508121.1; XM_006508058.2.
DR RefSeq; XP_006508122.1; XM_006508059.2.
DR RefSeq; XP_006508125.1; XM_006508062.1.
DR RefSeq; XP_017177877.1; XM_017322388.1.
DR AlphaFoldDB; Q9WTW2; -.
DR SMR; Q9WTW2; -.
DR STRING; 10090.ENSMUSP00000130019; -.
DR TCDB; 8.A.10.3.1; the slow voltage-gated k+) channel accessory protein (mink) family.
DR GlyGen; Q9WTW2; 3 sites.
DR iPTMnet; Q9WTW2; -.
DR PhosphoSitePlus; Q9WTW2; -.
DR PaxDb; Q9WTW2; -.
DR PRIDE; Q9WTW2; -.
DR ProteomicsDB; 301772; -.
DR Antibodypedia; 2753; 155 antibodies from 28 providers.
DR DNASU; 57442; -.
DR Ensembl; ENSMUST00000049333; ENSMUSP00000039353; ENSMUSG00000035165.
DR Ensembl; ENSMUST00000170954; ENSMUSP00000130019; ENSMUSG00000035165.
DR Ensembl; ENSMUST00000178946; ENSMUSP00000136616; ENSMUSG00000035165.
DR Ensembl; ENSMUST00000179842; ENSMUSP00000136415; ENSMUSG00000035165.
DR Ensembl; ENSMUST00000207358; ENSMUSP00000146583; ENSMUSG00000035165.
DR Ensembl; ENSMUST00000207995; ENSMUSP00000147151; ENSMUSG00000035165.
DR Ensembl; ENSMUST00000208260; ENSMUSP00000147047; ENSMUSG00000035165.
DR GeneID; 57442; -.
DR KEGG; mmu:57442; -.
DR UCSC; uc009imm.2; mouse.
DR CTD; 10008; -.
DR MGI; MGI:1891124; Kcne3.
DR VEuPathDB; HostDB:ENSMUSG00000035165; -.
DR eggNOG; ENOG502S4UF; Eukaryota.
DR GeneTree; ENSGT00940000155001; -.
DR HOGENOM; CLU_180169_0_0_1; -.
DR InParanoid; Q9WTW2; -.
DR OMA; AYMYILF; -.
DR OrthoDB; 1608208at2759; -.
DR PhylomeDB; Q9WTW2; -.
DR TreeFam; TF335981; -.
DR Reactome; R-MMU-5576890; Phase 3 - rapid repolarisation.
DR Reactome; R-MMU-5576893; Phase 2 - plateau phase.
DR BioGRID-ORCS; 57442; 2 hits in 72 CRISPR screens.
DR PRO; PR:Q9WTW2; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q9WTW2; protein.
DR Bgee; ENSMUSG00000035165; Expressed in spermatid and 114 other tissues.
DR ExpressionAtlas; Q9WTW2; baseline and differential.
DR Genevisible; Q9WTW2; MM.
DR GO; GO:1990794; C:basolateral part of cell; IDA:MGI.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0030425; C:dendrite; ISS:UniProtKB.
DR GO; GO:0045121; C:membrane raft; ISO:MGI.
DR GO; GO:0032809; C:neuronal cell body membrane; ISS:UniProtKB.
DR GO; GO:0043204; C:perikaryon; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0031982; C:vesicle; ISS:UniProtKB.
DR GO; GO:0008076; C:voltage-gated potassium channel complex; ISS:MGI.
DR GO; GO:0005267; F:potassium channel activity; ISS:MGI.
DR GO; GO:0015459; F:potassium channel regulator activity; ISS:UniProtKB.
DR GO; GO:0044325; F:transmembrane transporter binding; ISO:MGI.
DR GO; GO:0005249; F:voltage-gated potassium channel activity; IEA:InterPro.
DR GO; GO:0030644; P:cellular chloride ion homeostasis; IDA:MGI.
DR GO; GO:0086011; P:membrane repolarization during action potential; IBA:GO_Central.
DR GO; GO:0098915; P:membrane repolarization during ventricular cardiac muscle cell action potential; IEA:GOC.
DR GO; GO:1902260; P:negative regulation of delayed rectifier potassium channel activity; ISS:UniProtKB.
DR GO; GO:1905025; P:negative regulation of membrane repolarization during ventricular cardiac muscle cell action potential; ISO:MGI.
DR GO; GO:1903765; P:negative regulation of potassium ion export across plasma membrane; ISO:MGI.
DR GO; GO:1903817; P:negative regulation of voltage-gated potassium channel activity; ISO:MGI.
DR GO; GO:1901387; P:positive regulation of voltage-gated calcium channel activity; ISO:MGI.
DR GO; GO:0097623; P:potassium ion export across plasma membrane; IBA:GO_Central.
DR GO; GO:0071805; P:potassium ion transmembrane transport; IDA:MGI.
DR GO; GO:0086091; P:regulation of heart rate by cardiac conduction; ISO:MGI.
DR GO; GO:0043266; P:regulation of potassium ion transport; ISO:MGI.
DR GO; GO:0060307; P:regulation of ventricular cardiac muscle cell membrane repolarization; IBA:GO_Central.
DR GO; GO:0006814; P:sodium ion transport; IDA:MGI.
DR GO; GO:0086005; P:ventricular cardiac muscle cell action potential; IBA:GO_Central.
DR InterPro; IPR000369; K_chnl_KCNE.
DR InterPro; IPR005426; K_chnl_volt-dep_bsu_KCNE3.
DR PANTHER; PTHR15282; PTHR15282; 1.
DR PANTHER; PTHR15282:SF6; PTHR15282:SF6; 1.
DR Pfam; PF02060; ISK_Channel; 1.
DR PRINTS; PR01606; KCNE3CHANNEL.
DR PRINTS; PR00168; KCNECHANNEL.
PE 3: Inferred from homology;
KW Cell membrane; Cell projection; Cytoplasm; Glycoprotein; Ion channel;
KW Ion transport; Membrane; Potassium; Potassium channel; Potassium transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport;
KW Voltage-gated channel.
FT CHAIN 1..103
FT /note="Potassium voltage-gated channel subfamily E member
FT 3"
FT /id="PRO_0000144290"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 79..103
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 30..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 5
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 22
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 41
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 103 AA; 11720 MW; 92E7C8C2284A8804 CRC64;
METSNGTETW YMSLHAVLKA LNTTLHSHLL CRPGPGPGPD NQTEDRRASL PGRNDNSYMY
ILFVMFLFAV TVGSLILGYT RSRKVDKRSD PYHVYIKNRV SMI
