ID   KCNKC_RAT               Reviewed;         430 AA.
AC   Q9ERS1;
DT   01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Potassium channel subfamily K member 12;
DE   AltName: Full=Tandem pore domain halothane-inhibited potassium channel 2;
DE            Short=THIK-2;
GN   Name=Kcnk12;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   STRAIN=Wistar; TISSUE=Brain;
RX   PubMed=11060316; DOI=10.1074/jbc.m008985200;
RA   Rajan S., Wischmeyer E., Karschin C., Preisig-Mueller R., Grzeschik K.-H.,
RA   Daut J., Karschin A., Derst C.;
RT   "THIK-1 and THIK-2, a novel subfamily of tandem pore domain K+ channels.";
RL   J. Biol. Chem. 276:7302-7311(2001).
CC   -!- FUNCTION: Probable potassium channel subunit. No channel activity
CC       observed in heterologous systems. May need to associate with another
CC       protein to form a functional channel.
CC   -!- SUBUNIT: Heterodimer. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in most brain regions. Also
CC       expressed in other tissues such as lung, kidney, liver, stomach and
CC       spleen. {ECO:0000269|PubMed:11060316}.
CC   -!- SIMILARITY: Belongs to the two pore domain potassium channel (TC
CC       1.A.1.8) family. {ECO:0000305}.
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DR   EMBL; AF287300; AAG32311.1; -; mRNA.
DR   RefSeq; NP_071628.1; NM_022292.1.
DR   AlphaFoldDB; Q9ERS1; -.
DR   SMR; Q9ERS1; -.
DR   STRING; 10116.ENSRNOP00000021570; -.
DR   GlyGen; Q9ERS1; 1 site.
DR   PaxDb; Q9ERS1; -.
DR   ABCD; Q9ERS1; 1 sequenced antibody.
DR   Ensembl; ENSRNOT00000021570; ENSRNOP00000021570; ENSRNOG00000016110.
DR   GeneID; 64119; -.
DR   KEGG; rno:64119; -.
DR   UCSC; RGD:68940; rat.
DR   CTD; 56660; -.
DR   RGD; 68940; Kcnk12.
DR   eggNOG; KOG4404; Eukaryota.
DR   GeneTree; ENSGT00940000161424; -.
DR   HOGENOM; CLU_022504_3_1_1; -.
DR   InParanoid; Q9ERS1; -.
DR   OMA; VEGWNYV; -.
DR   OrthoDB; 645335at2759; -.
DR   PhylomeDB; Q9ERS1; -.
DR   TreeFam; TF313947; -.
DR   Reactome; R-RNO-5576886; Phase 4 - resting membrane potential.
DR   PRO; PR:Q9ERS1; -.
DR   Proteomes; UP000002494; Chromosome 6.
DR   Bgee; ENSRNOG00000016110; Expressed in testis and 7 other tissues.
DR   Genevisible; Q9ERS1; RN.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0015271; F:outward rectifier potassium channel activity; IBA:GO_Central.
DR   GO; GO:0022841; F:potassium ion leak channel activity; IBA:GO_Central.
DR   GO; GO:0071805; P:potassium ion transmembrane transport; IBA:GO_Central.
DR   GO; GO:0034765; P:regulation of ion transmembrane transport; IEA:UniProtKB-KW.
DR   GO; GO:0030322; P:stabilization of membrane potential; IBA:GO_Central.
DR   InterPro; IPR003280; 2pore_dom_K_chnl.
DR   InterPro; IPR005410; 2pore_dom_K_chnl_THIK.
DR   InterPro; IPR013099; K_chnl_dom.
DR   PANTHER; PTHR11003; PTHR11003; 1.
DR   Pfam; PF07885; Ion_trans_2; 2.
DR   PRINTS; PR01333; 2POREKCHANEL.
DR   PRINTS; PR01588; THIKCHANNEL.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Ion channel; Ion transport; Membrane; Potassium;
KW   Potassium channel; Potassium transport; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport; Voltage-gated channel.
FT   CHAIN           1..430
FT                   /note="Potassium channel subfamily K member 12"
FT                   /id="PRO_0000101761"
FT   TOPO_DOM        1..38
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   INTRAMEM        114..134
FT                   /note="Pore-forming; Name=Pore-forming 1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        145..165
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        166..212
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        213..233
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   INTRAMEM        243..263
FT                   /note="Pore-forming; Name=Pore-forming 2"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        282..302
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        303..430
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        78
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   430 AA;  46965 MW;  69F1D16C6BFE7180 CRC64;
     MSSRSPRPPP RRCRRRLPRP SCCCCCCRRS HLNEDTGRFV LLAALIGLYL VAGATVFSAL
     ESPGEAEARA RWGATLRNFS AAHGVAEPEL RAFLRHYEAA LAAGVRADAL RPRWDFPGAF
     YFVGTVVSTI GFGMTTPATV GGKAFLIAYG LFGCAGTILF FNLFLERIIS LLAFIMRACR
     ERQLRRSGLL PATFRRGSAL SEADSLAGWK PSVYHVLLIL GLFAVLLACC ASAMYTSVEG
     WDYVDSLYFC FVTFSTIGFG DLVSSQHAAY RNQGLYRLGN FLFILLGVCC IYSLFNVISI
     LIKQVLNWML RKLSCRCCTR CCPAPGAPLS RRNAITPGSR LRRRLAALGT DPAARDSDAE
     GRRLSGELIS MRDLTASNKV SLALLQKQLS ETANGYPRSV CVNTRQNGFS GGVGALGIMN
     NRLAETSASR