KCNKF_HUMAN
ID KCNKF_HUMAN Reviewed; 330 AA.
AC Q9H427; Q52LL3; Q9HBC8;
DT 01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 23-FEB-2022, sequence version 3.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Potassium channel subfamily K member 15;
DE AltName: Full=Acid-sensitive potassium channel protein TASK-5;
DE AltName: Full=TWIK-related acid-sensitive K(+) channel 5;
DE AltName: Full=Two pore potassium channel KT3.3;
DE Short=Two pore K(+) channel KT3.3;
GN Name=KCNK15; Synonyms=TASK5;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=11409881; DOI=10.1006/bbrc.2001.5064;
RA Kim D., Gnatenco C.;
RT "TASK-5, a new member of the tandem-pore K(+) channel family.";
RL Biochem. Biophys. Res. Commun. 284:923-930(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS GLY-95; PRO-260 AND PRO-323.
RC TISSUE=Brain;
RX PubMed=11431495; DOI=10.1152/jn.2001.86.1.130;
RA Vega-Saenz de Miera E., Lau D.H.P., Zhadina M., Pountney D., Coetzee W.A.,
RA Rudy B.;
RT "KT3.2 and KT3.3, two novel human two-pore K(+) channels closely related to
RT TASK-1.";
RL J. Neurophysiol. 86:130-142(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], POLYMORPHISM, VARIANTS GLY-95;
RP PRO-260; HIS-261 AND PRO-323, AND MUTAGENESIS OF ARG-138; 141-LEU--CYS-145;
RP TRP-151 AND CYS-153.
RC TISSUE=Brain, Colon, and Ovarian carcinoma;
RX PubMed=11749039; DOI=10.1006/mcne.2001.1045;
RA Karschin C., Wischmeyer E., Preisig-Mueller R., Rajan S., Derst C.,
RA Grzeschik K.-H., Daut J., Karschin A.;
RT "Expression pattern in brain of TASK-1, TASK-3, and a tandem pore domain
RT K(+) channel subunit, TASK-5, associated with the central auditory nervous
RT system.";
RL Mol. Cell. Neurosci. 18:632-648(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA], VARIANTS GLY-95; PRO-260 AND PRO-323, AND
RP TISSUE SPECIFICITY.
RX PubMed=11680614; DOI=10.1007/s004240100620;
RA Ashmole I., Goodwin P.A., Stanfield P.R.;
RT "TASK-5, a novel member of the tandem pore K+ channel family.";
RL Pflugers Arch. 442:828-833(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11780052; DOI=10.1038/414865a;
RA Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R.,
RA Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L.,
RA Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P.,
RA Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., Buck D., Burrill W.D.,
RA Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G.,
RA Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E.,
RA Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D.,
RA Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P.,
RA Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E.,
RA Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J.,
RA Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D.,
RA Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S.,
RA Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D.,
RA Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A.,
RA Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T.,
RA Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I.,
RA Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M.,
RA Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D.,
RA Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M.,
RA Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A.,
RA Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L.,
RA Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L.,
RA Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 20.";
RL Nature 414:865-871(2001).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Probable potassium channel subunit. No channel activity
CC observed in heterologous systems. May need to associate with another
CC protein to form a functional channel.
CC -!- SUBUNIT: Heterodimer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Detected in pancreas, heart, placenta, lung, liver,
CC kidney, ovary, testis, skeletal muscle and adrenal gland, and at lower
CC levels in prostate, spleen and thyroid gland.
CC {ECO:0000269|PubMed:11409881, ECO:0000269|PubMed:11680614}.
CC -!- POLYMORPHISM: Three alleles are known: TASK-5A, TASK-5B and TASK-5C.
CC The sequence shown is that of allele TASK-5B.
CC {ECO:0000269|PubMed:11409881, ECO:0000269|PubMed:11749039,
CC ECO:0000269|PubMed:11780052, ECO:0000269|PubMed:15489334}.
CC -!- SIMILARITY: Belongs to the two pore domain potassium channel (TC
CC 1.A.1.8) family. {ECO:0000305}.
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DR EMBL; AF257081; AAG33127.1; -; mRNA.
DR EMBL; AF294350; AAK97091.1; -; mRNA.
DR EMBL; AF294351; AAK97092.1; -; mRNA.
DR EMBL; AF294352; AAK97093.1; -; Genomic_DNA.
DR EMBL; AF336342; AAK37518.1; -; mRNA.
DR EMBL; AL118522; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC093874; AAH93874.1; -; mRNA.
DR EMBL; BC143282; AAI43283.1; -; mRNA.
DR CCDS; CCDS13337.1; -.
DR PIR; JC7703; JC7703.
DR RefSeq; NP_071753.2; NM_022358.3.
DR AlphaFoldDB; Q9H427; -.
DR STRING; 9606.ENSP00000361952; -.
DR TCDB; 1.A.1.9.12; the voltage-gated ion channel (vic) superfamily.
DR PhosphoSitePlus; Q9H427; -.
DR BioMuta; KCNK15; -.
DR DMDM; 24636282; -.
DR MassIVE; Q9H427; -.
DR MaxQB; Q9H427; -.
DR PaxDb; Q9H427; -.
DR PeptideAtlas; Q9H427; -.
DR PRIDE; Q9H427; -.
DR ProteomicsDB; 80784; -.
DR Antibodypedia; 27447; 93 antibodies from 20 providers.
DR DNASU; 60598; -.
DR Ensembl; ENST00000372861.5; ENSP00000361952.3; ENSG00000124249.7.
DR GeneID; 60598; -.
DR KEGG; hsa:60598; -.
DR MANE-Select; ENST00000372861.5; ENSP00000361952.3; NM_022358.4; NP_071753.2.
DR UCSC; uc002xmr.4; human.
DR CTD; 60598; -.
DR DisGeNET; 60598; -.
DR GeneCards; KCNK15; -.
DR HGNC; HGNC:13814; KCNK15.
DR HPA; ENSG00000124249; Low tissue specificity.
DR MIM; 607368; gene.
DR neXtProt; NX_Q9H427; -.
DR OpenTargets; ENSG00000124249; -.
DR PharmGKB; PA30056; -.
DR VEuPathDB; HostDB:ENSG00000124249; -.
DR eggNOG; KOG4404; Eukaryota.
DR GeneTree; ENSGT00940000160902; -.
DR HOGENOM; CLU_022504_4_2_1; -.
DR InParanoid; Q9H427; -.
DR OrthoDB; 1109218at2759; -.
DR PhylomeDB; Q9H427; -.
DR TreeFam; TF313947; -.
DR PathwayCommons; Q9H427; -.
DR Reactome; R-HSA-5576886; Phase 4 - resting membrane potential.
DR SignaLink; Q9H427; -.
DR BioGRID-ORCS; 60598; 8 hits in 1057 CRISPR screens.
DR ChiTaRS; KCNK15; human.
DR GeneWiki; KCNK15; -.
DR GenomeRNAi; 60598; -.
DR Pharos; Q9H427; Tbio.
DR PRO; PR:Q9H427; -.
DR Proteomes; UP000005640; Chromosome 20.
DR RNAct; Q9H427; protein.
DR Bgee; ENSG00000124249; Expressed in thoracic aorta and 89 other tissues.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0022841; F:potassium ion leak channel activity; IBA:GO_Central.
DR GO; GO:0071805; P:potassium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0034765; P:regulation of ion transmembrane transport; IEA:UniProtKB-KW.
DR GO; GO:0030322; P:stabilization of membrane potential; IBA:GO_Central.
DR InterPro; IPR003280; 2pore_dom_K_chnl.
DR InterPro; IPR003092; 2pore_dom_K_chnl_TASK.
DR InterPro; IPR013099; K_chnl_dom.
DR InterPro; IPR008073; TASK5.
DR PANTHER; PTHR11003; PTHR11003; 1.
DR Pfam; PF07885; Ion_trans_2; 2.
DR PIRSF; PIRSF038061; K_channel_subfamily_K_type; 1.
DR PRINTS; PR01333; 2POREKCHANEL.
DR PRINTS; PR01690; TASK5CHANNEL.
DR PRINTS; PR01095; TASKCHANNEL.
PE 1: Evidence at protein level;
KW Ion channel; Ion transport; Membrane; Potassium; Potassium channel;
KW Potassium transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Voltage-gated channel.
FT CHAIN 1..330
FT /note="Potassium channel subfamily K member 15"
FT /id="PRO_0000101765"
FT TOPO_DOM 1..8
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT INTRAMEM 80..101
FT /note="Pore-forming; Name=Pore-forming 1"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 129..158
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT INTRAMEM 189..209
FT /note="Pore-forming; Name=Pore-forming 2"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 244..330
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 256..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 95
FT /note="E -> G (in allele TASK-5A and allele TASK-5C;
FT dbSNP:rs1111032)"
FT /evidence="ECO:0000269|PubMed:11431495,
FT ECO:0000269|PubMed:11749039"
FT /id="VAR_014211"
FT VARIANT 260
FT /note="T -> P (in allele TASK-5A and allele TASK-5C;
FT dbSNP:rs6073538)"
FT /evidence="ECO:0000269|PubMed:11431495,
FT ECO:0000269|PubMed:11749039"
FT /id="VAR_014212"
FT VARIANT 261
FT /note="P -> H (in allele TASK-5A; dbSNP:rs13037900)"
FT /evidence="ECO:0000269|PubMed:11749039,
FT ECO:0000269|PubMed:15489334"
FT /id="VAR_014213"
FT VARIANT 323
FT /note="L -> P (in allele TASK-5A and allele TASK-5C;
FT dbSNP:rs13042905)"
FT /evidence="ECO:0000269|PubMed:11431495,
FT ECO:0000269|PubMed:11749039"
FT /id="VAR_014214"
FT MUTAGEN 138
FT /note="R->Y: No effect on lack of functional expression."
FT /evidence="ECO:0000269|PubMed:11749039"
FT MUTAGEN 141..145
FT /note="LAAKC->HRAKK: No effect on lack of functional
FT expression."
FT /evidence="ECO:0000269|PubMed:11749039"
FT MUTAGEN 151
FT /note="W->R: No effect on lack of functional expression."
FT /evidence="ECO:0000269|PubMed:11749039"
FT MUTAGEN 153
FT /note="C->D: No effect on lack of functional expression."
FT /evidence="ECO:0000269|PubMed:11749039"
SQ SEQUENCE 330 AA; 36222 MW; 24F428721A1C7790 CRC64;
MRRPSVRAAG LVLCTLCYLL VGAAVFDALE SEAESGRQRL LVQKRGALRR KFGFSAEDYR
ELERLALQAE PHRAGRQWKF PGSFYFAITV ITTIEYGHAA PGTDSGKVFC MFYALLGIPL
TLVTFQSLGE RLNAVVRRLL LAAKCCLGLR WTCVSTENLV VAGLLACAAT LALGAVAFSH
FEGWTFFHAY YYCFITLTTI GFGDFVALQS GEALQRKLPY VAFSFLYILL GLTVIGAFLN
LVVLRFLVAS ADWPERAART PSPRPPGAPE SRGLWLPRRP ARSVGSASVF CHVHKLERCA
RDNLGFSPPS SPGVVRGGQA PRLGARWKSI
