ID   APY1_ORYSJ              Reviewed;         489 AA.
AC   Q8H7L6;
DT   31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Probable apyrase 1;
DE            Short=OsAPY1;
DE            EC=3.6.1.5;
DE   AltName: Full=ATP-diphosphatase;
DE   AltName: Full=ATP-diphosphohydrolase;
DE   AltName: Full=Adenosine diphosphatase;
DE            Short=ADPase;
GN   Name=APY1; OrderedLocusNames=Os03g0328400, LOC_Os03g21120;
GN   ORFNames=OSJNBb0014I10.10;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
CC   -!- FUNCTION: Catalyzes the hydrolysis of phosphoanhydride bonds of
CC       nucleoside tri- and di-phosphates. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + 2 H2O = a ribonucleoside
CC         5'-phosphate + 2 H(+) + 2 phosphate; Xref=Rhea:RHEA:36795,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:58043, ChEBI:CHEBI:61557; EC=3.6.1.5;
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Single-pass type II
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GDA1/CD39 NTPase family. {ECO:0000305}.
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DR   EMBL; AC126222; AAN65004.1; -; Genomic_DNA.
DR   EMBL; DP000009; ABF95735.1; -; Genomic_DNA.
DR   EMBL; AP014959; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; XP_015632681.1; XM_015777195.1.
DR   AlphaFoldDB; Q8H7L6; -.
DR   SMR; Q8H7L6; -.
DR   STRING; 4530.OS03T0328400-00; -.
DR   PaxDb; Q8H7L6; -.
DR   PRIDE; Q8H7L6; -.
DR   GeneID; 4332721; -.
DR   KEGG; osa:4332721; -.
DR   InParanoid; Q8H7L6; -.
DR   OrthoDB; 1337265at2759; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0102490; F:8-oxo-dGTP phosphohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0102485; F:dATP phosphohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102486; F:dCTP phosphohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102491; F:dGTP phosphohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102488; F:dTTP phosphohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102487; F:dUTP phosphohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102489; F:GTP phosphohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0017110; F:nucleoside-diphosphatase activity; IBA:GO_Central.
DR   GO; GO:0009134; P:nucleoside diphosphate catabolic process; IBA:GO_Central.
DR   InterPro; IPR000407; GDA1_CD39_NTPase.
DR   PANTHER; PTHR11782; PTHR11782; 1.
DR   Pfam; PF01150; GDA1_CD39; 1.
DR   PROSITE; PS01238; GDA1_CD39_NTPASE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Calcium; Hydrolase; Membrane; Nucleotide-binding;
KW   Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix.
FT   CHAIN           1..489
FT                   /note="Probable apyrase 1"
FT                   /id="PRO_0000419907"
FT   TOPO_DOM        1..28
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        29..49
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        50..489
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        211
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         89..99
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000305"
FT   BINDING         235..245
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   489 AA;  52799 MW;  0BF6BF154488D38E CRC64;
     MRRFSAAAGA RQQQQQGEAV SDRVLRFRGV LVVVLAPVLL ISLVLLLMPR APASATVEGS
     AGELVAAAGR RWGPRAVSGL GDGSTRYAVI FDAGSSGSRV HVYCFDGNLD LLPIGKEIEL
     FKQKKPGLSA YAMDPQEAAK SLVSLLEEAE KVIPVELREQ TPVRVGATAG LRALGTEKSE
     EILQAVRDLL QDKSSFRSQP EWVTVLDGSQ EGAFQWVTIN YLLGNLGKPY SHTVGVVDLG
     GGSVQMAYAI SEKDAGKAPP VAEGEDSYVK ELLLKGTTYY LYVHSYLRYG LLAARAEILK
     AGEGNDYRNC MLEGHHGQYR YGDDIFEASG LSSGASYSKC RAVAVRALKV DEPACTHMKC
     TFGGVWNGGG GDGQKNLFVA SFFFDRAAEA GFVNPKAPFA KVKPSDFEEA ARRVCKLNVK
     DAQATYPDVS EENVPYLCMD LVYQYTLLVD GFGVDPYQDI TLVKKVPYSN SFVEAAWPLG
     SAIEVASSS