ID   KCP4_PINMG              Reviewed;         198 AA.
AC   H2A0N9;
DT   13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2012, sequence version 1.
DT   03-AUG-2022, entry version 31.
DE   RecName: Full=BPTI/Kunitz domain-containing protein 4;
DE   AltName: Full=Nacre serine protease inhibitor 3;
DE            Short=NSPI3;
DE   Flags: Precursor;
OS   Margaritifera margaritifera (Freshwater pearl mussel).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Bivalvia;
OC   Autobranchia; Pteriomorphia; Pterioida; Pterioidea; Pteriidae; Pinctada.
OX   NCBI_TaxID=102329;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND IDENTIFICATION.
RC   TISSUE=Mantle;
RX   PubMed=21040589; DOI=10.1186/1471-2164-11-613;
RA   Joubert C., Piquemal D., Marie B., Manchon L., Pierrat F.,
RA   Zanella-Cleon I., Cochennec-Laureau N., Gueguen Y., Montagnani C.;
RT   "Transcriptome and proteome analysis of Pinctada margaritifera calcifying
RT   mantle and shell: focus on biomineralization.";
RL   BMC Genomics 11:613-613(2010).
RN   [2]
RP   PROTEIN SEQUENCE OF 130-150 AND 162-170, SUBCELLULAR LOCATION, AND TISSUE
RP   SPECIFICITY.
RC   TISSUE=Shell;
RX   PubMed=23213212; DOI=10.1073/pnas.1210552109;
RA   Marie B., Joubert C., Tayale A., Zanella-Cleon I., Belliard C.,
RA   Piquemal D., Cochennec-Laureau N., Marin F., Gueguen Y., Montagnani C.;
RT   "Different secretory repertoires control the biomineralization processes of
RT   prism and nacre deposition of the pearl oyster shell.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:20986-20991(2012).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23213212}.
CC   -!- TISSUE SPECIFICITY: Nacreous layer of shell (at protein level).
CC       {ECO:0000269|PubMed:23213212}.
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DR   EMBL; HE610406; CCE46180.1; -; mRNA.
DR   MEROPS; I02.959; -.
DR   PRIDE; H2A0N9; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00109; KU; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR004094; Antistasin-like.
DR   InterPro; IPR011061; Hirudin/antistatin.
DR   InterPro; IPR002223; Kunitz_BPTI.
DR   InterPro; IPR036880; Kunitz_BPTI_sf.
DR   InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR   Pfam; PF02822; Antistasin; 2.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   PRINTS; PR00759; BASICPTASE.
DR   SMART; SM00131; KU; 1.
DR   SUPFAM; SSF57262; SSF57262; 2.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   PROSITE; PS51252; ANTISTASIN; 4.
DR   PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor; Repeat;
KW   Secreted; Serine protease inhibitor; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..198
FT                   /note="BPTI/Kunitz domain-containing protein 4"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000417940"
FT   DOMAIN          31..56
FT                   /note="Antistasin-like 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00582"
FT   DOMAIN          56..85
FT                   /note="Antistasin-like 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00582"
FT   DOMAIN          85..114
FT                   /note="Antistasin-like 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00582"
FT   DOMAIN          114..143
FT                   /note="Antistasin-like 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00582"
FT   DOMAIN          146..196
FT                   /note="BPTI/Kunitz inhibitor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        146..196
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        155..179
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        171..192
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
SQ   SEQUENCE   198 AA;  22096 MW;  5E4CBC92ED76C1F7 CRC64;
     MNLLVFFVYL XVCVIGSHSI XGSQQLSTKK CGGAICAMAC TYGFVLDKDG CPICKCRDAP
     CPLVKCLPCK YGYVIDDNGC QTCECKPLDC GLVCLIYCPY GNIPDERGCP TCFCRPRPCK
     ELECEKKCRN NVLDEIGCPT CKCKECLEPK KVGPCRALIP RYFYDVWSGK CKKFYWGGCQ
     ANGNNFKRKS QCCKRCKS