KCP_XENLA
ID KCP_XENLA Reviewed; 2327 AA.
AC Q9IBG7;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Kielin/chordin-like protein;
DE AltName: Full=Cysteine-rich motor neuron 2 protein;
DE Short=CRIM-2;
DE AltName: Full=Kielin;
DE Flags: Precursor;
GN Name=kcp; Synonyms=crim2;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND
RP FUNCTION.
RC TISSUE=Embryonic floor plate;
RX PubMed=10779551; DOI=10.1073/pnas.090020497;
RA Matsui M., Mizuseki K., Nakatani J., Nakanishi S., Sasai Y.;
RT "Xenopus kielin: A dorsalizing factor containing multiple chordin-type
RT repeats secreted from the embryonic midline.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:5291-5296(2000).
CC -!- FUNCTION: May be a signaling molecule that mediates inductive
CC activities of the embryonic midline. Able to dorsalize mesoderm.
CC {ECO:0000269|PubMed:10779551}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10779551}.
CC -!- DEVELOPMENTAL STAGE: Expression starts at the mid-gastrula stage in the
CC dorsal midline. Expressed in the axial mesoderm at this stage. At
CC neurula stages, expression is detected both in the axial mesoderm
CC (notochord and prechordal mesoderm) and in the ventral central nervous
CC system (floor plate and ventral forebrain). At tailbud stages,
CC expression is diminished in the notochord and remains in the ventral
CC central nervous system (CNS). During and after tailbud stages, other
CC regions shows detectable levels of expression. The epiphyseal placode
CC has a strong expression. Additional expression is found in dorsal parts
CC of the CNS, especially in the anterior spinal cord and hindbrain as
CC well as in the tailbud mesoderm. At the larval stage, expression is
CC also found in the forming heart. Induced in mesoderm and in ectoderm by
CC nodal-related genes. {ECO:0000269|PubMed:10779551}.
CC -!- MISCELLANEOUS: 'Kiel' means keel in English. As the expression in the
CC central nervous system (CNS) resembled a keel of ship, the gene was
CC named Kielin.
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DR EMBL; AB026192; BAA95483.1; -; mRNA.
DR RefSeq; NP_001079355.1; NM_001085886.1.
DR AlphaFoldDB; Q9IBG7; -.
DR SMR; Q9IBG7; -.
DR GeneID; 378806; -.
DR KEGG; xla:378806; -.
DR CTD; 378806; -.
DR Xenbase; XB-GENE-865639; kcp.L.
DR Proteomes; UP000186698; Chromosome 3L.
DR Bgee; 378806; Expressed in neurula embryo and 15 other tissues.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001791; Laminin_G.
DR InterPro; IPR036084; Ser_inhib-like_sf.
DR InterPro; IPR014853; Unchr_dom_Cys-rich.
DR InterPro; IPR001007; VWF_dom.
DR InterPro; IPR001846; VWF_type-D.
DR Pfam; PF00093; VWC; 16.
DR Pfam; PF00094; VWD; 1.
DR SMART; SM00832; C8; 1.
DR SMART; SM00210; TSPN; 1.
DR SMART; SM00214; VWC; 28.
DR SMART; SM00215; VWC_out; 12.
DR SMART; SM00216; VWD; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR SUPFAM; SSF57567; SSF57567; 1.
DR PROSITE; PS01208; VWFC_1; 20.
DR PROSITE; PS50184; VWFC_2; 23.
DR PROSITE; PS51233; VWFD; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Disulfide bond; Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..2327
FT /note="Kielin/chordin-like protein"
FT /id="PRO_0000318588"
FT DOMAIN 339..400
FT /note="VWFC 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 401..438
FT /note="VWFC 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 439..493
FT /note="VWFC 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 494..553
FT /note="VWFC 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 554..610
FT /note="VWFC 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 611..669
FT /note="VWFC 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 670..728
FT /note="VWFC 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 729..786
FT /note="VWFC 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 787..847
FT /note="VWFC 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 848..907
FT /note="VWFC 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 908..966
FT /note="VWFC 11"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 967..1025
FT /note="VWFC 12"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 1026..1083
FT /note="VWFC 13"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 1084..1142
FT /note="VWFC 14"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 1146..1203
FT /note="VWFC 15"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 1204..1260
FT /note="VWFC 16"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 1261..1319
FT /note="VWFC 17"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 1321..1377
FT /note="VWFC 18"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 1378..1439
FT /note="VWFC 19"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 1440..1495
FT /note="VWFC 20"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 1496..1555
FT /note="VWFC 21"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 1556..1614
FT /note="VWFC 22"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 1615..1673
FT /note="VWFC 23"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 1674..1731
FT /note="VWFC 24"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 1732..1799
FT /note="VWFC 25"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 1800..1860
FT /note="VWFC 26"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 1861..1924
FT /note="VWFC 27"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 1928..1988
FT /note="VWFC 28"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT DOMAIN 1992..2168
FT /note="VWFD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00580"
FT DOMAIN 2259..2319
FT /note="TIL"
FT REGION 250..294
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 291..332
FT /evidence="ECO:0000255"
FT COMPBIAS 259..277
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 1994..2126
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00580"
FT DISULFID 2016..2167
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00580"
SQ SEQUENCE 2327 AA; 255802 MW; 0293109329209983 CRC64;
MNTLLWTILL PLLFSFCVCQ QPEHQDLEMS VQYYDDNVID LLEALNVTRS VKGVTKAKGS
DPASPAWKFR QRVPHLTLPR DYSVYLLSTT QESLGLHFVA KQAKNNRGTL VAFLSPAATK
IDGRPLLRLI SDTHTDQLYF EYRTAQTMEP ASLHFPGSSP FSGSQWARVA LNVNTHKVTL
FLDCEEPVVF GKEGAEEMLS LILPLDLEIT FASTPSDKES KFLGYWQTAE ISPTGFTRRP
WHCENRSDSL PLPYSLSGER QMEDEEIQRE PRAPDLSDTD HYQQQQSEVP AQLLAKDDRL
QRLEEAVKGL TNMIDMIKSQ NADLQARVIA LESCECRRST CVWEDKEYQD SETWKKDACN
ICVCVGGSVT CSVRKDWPQC LGCFHEGRNY NNKDIFSVGP CMSCICQSGE VSCTPKLCPP
VTCSDPVTLP NECCPLCATG CSDGHKEGDT WRKDTCTTCT CQNGTISCER EQCPELTCLK
RHTPPGQCCA KCQQGCEYEG LIYRNGDYFL SQSNPCVNCS CLNNLVRCLP VQCPLPACTN
PVPIPGQCCP SCPVCELDGH PLIPGQNVTT KDGCRLCSCQ DGKVQCTESV QCPHICTHGV
RSNSCCLDCS ACEMHGDIIP NGLTFQGNMD PCESCTCQDG NVHCVRVSCP ELSCVLHEKI
PGECCSQCQS CMDGTVKRKH GEEWKPQGDP CQSCRCLEGR VQCRKRHCAA LCRNPLPPRP
GTCCPMCDGC LYNGRSYLNG QPVRSTDQCN RCFCENGNVQ CEPIACPQAP CRNPVRRTGE
CCPRCEGCEY DSRHFAEGVV FTTAHDPCLQ CTCLSGEVSC EHLDRKCPPS QCSHPGKAAG
QCCPSCDVCD FEGILYTDRQ TFQPPGHGPC LKCFCTIGNV RCVEETCPPA PCPNPVRDPE
QCCPVCKVCV QDGVEFLEGI EWELDGNPCS SCTCRNGDTV CGVSECPPVS CLHPTRREGE
CCPVCDSCSY NQRLYSNEQI FTDPDNPCQD CQCKDGTVQC SSIVCPPVLC TIPERTPGQC
CAKCPDCRYQ DQIFLEGEQF SNPLNQCQEC WCRDGHVTCT DRGCTGALCS YPLPGTCCQN
NCNGCNYAGK EYPNGADFPH PTDKCRQCHC INGNVQCLAQ RCPPLLCAEP FPVPGECCPQ
CPVPPADCPY SGVTYRHMQR FYDPSDKCRD CICNNGTVTC QRKPCAPTPC LHPLQGDCCR
SCDGCLMSGK ELANGEQFPQ PSDPCSVCVC WEGSVTCQPK TCPVLNCPFP APGQCCKECQ
DCQYFGEVYL NGQEFSAPED SCSRCVCADG FVTCSKKPCY KAGCTHPSTP PGKCCPVCDG
CSYNGDALIN SQSVPDPSNP LCSECTCRAG SVQCVRKLCG PTSCPHPVTG PCDCPICQGC
HFQGHNYIDG EVFTSAQSQC EQCRCMRGHV TCGPRPCDQV TCPHPAEDPC MCPVCDGCNY
SGRDCTNGES FPDPEDECSH CTCRNGEVAC ISVPCPRVSC MYPITPRGEC CPRCTGICKH
NGRVYQSGDT FHPPGDLCTK CSCQNEMVNC QRVRCSQECS HPVLSPASSC CPVCDRCFYE
NREYANHETF TSTSDPCQRC VCLDGSVTCT HVVCPYVSCA NPITKPGQCC RECPVCRYQG
KEFSEGAHWV PHTDPCLKCT CSNGHVDCEP PQCPPLPCTQ QVTDPGTCCP RCRGCVYNGR
EYRDNSNWLS SSDHCMSCMC VDGVTTCSKL QCITSCTNQI TIPGECCPVC ADCISNSKVY
LPGDSYNPSK DPCEICTCES LPNGQQYRHC TKKQCPSLLD CPRSYILPPA EGQCCSSCAQ
ALSNCTNTLV GNEIQATDDP CYTCHCKDLT WVCVHQPCPA LSCPRSEQFT HSGSCCPVCN
ECVVEIEGRR VPDGETWTDR QDPCVTCTCT LGHVECQIEE CQPVQCQEGE RKVKRPGTCC
HECQASAVSC WYQGQRFLSN EHWQVDECTA CTCVSGEVHC HSERCPQVSC TAEETPALIP
GMCCPHCIPR PATCIAFGDP HYRTFDGKMY HFQGSCTYVL SEDCEGGDFS IHVTNDDRGL
RGVSWTKEVT VLIGDAVVQL LQDWVVMVDY QTVELPFLKE PYIYIERKTN TILLNSNIGV
KVQWNGRSHL EVSVPGTYRD HLCGLCGNFN NYPQDDLRDR RGQILMSEAA FGNSWRVQSS
NDSSSSCWDG QDVDPCKQAG YRARKEANGR CKLLKSSVFE PCHRVVPPEM FFASCVYDLC
ACGAGDECLC DVLEAYASEC REAGVILQWR SPALCAVGCP HDRGYVFDEC GPPCPKTCFN
KDVPLGVLES HCFKPCVPGC QCPAGLVEHE SHCIPPESCP KIIHGNL
