KCTD3_MOUSE
ID KCTD3_MOUSE Reviewed; 815 AA.
AC Q8BFX3; Q6P7X4;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=BTB/POZ domain-containing protein KCTD3;
GN Name=Kctd3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Ovary;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH HCN3, AND TISSUE
RP SPECIFICITY.
RX PubMed=23382386; DOI=10.1074/jbc.m112.434803;
RA Cao-Ehlker X., Zong X., Hammelmann V., Gruner C., Fenske S., Michalakis S.,
RA Wahl-Schott C., Biel M.;
RT "Up-regulation of hyperpolarization-activated cyclic nucleotide-gated
RT channel 3 (HCN3) by specific interaction with K+ channel tetramerization
RT domain-containing protein 3 (KCTD3).";
RL J. Biol. Chem. 288:7580-7589(2013).
CC -!- FUNCTION: Accessory subunit of potassium/sodium hyperpolarization-
CC activated cyclic nucleotide-gated channel 3 (HCN3) up-regulating its
CC cell-surface expression and current density without affecting its
CC voltage dependence and kinetics. {ECO:0000269|PubMed:23382386}.
CC -!- SUBUNIT: Interacts with HCN3. {ECO:0000269|PubMed:23382386}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23382386}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BFX3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BFX3-2; Sequence=VSP_020070, VSP_020071;
CC -!- TISSUE SPECIFICITY: Brain (at protein level). Expressed in the liver
CC kidney, and heart. {ECO:0000269|PubMed:23382386}.
CC -!- SIMILARITY: Belongs to the KCTD3 family. {ECO:0000305}.
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DR EMBL; AK049362; BAC33709.1; -; mRNA.
DR EMBL; AK087865; BAC40028.1; -; mRNA.
DR EMBL; BC061458; AAH61458.1; -; mRNA.
DR EMBL; BC079581; AAH79581.1; -; mRNA.
DR CCDS; CCDS15608.1; -. [Q8BFX3-1]
DR RefSeq; NP_766238.1; NM_172650.2. [Q8BFX3-1]
DR AlphaFoldDB; Q8BFX3; -.
DR SMR; Q8BFX3; -.
DR BioGRID; 230557; 2.
DR STRING; 10090.ENSMUSP00000082821; -.
DR iPTMnet; Q8BFX3; -.
DR PhosphoSitePlus; Q8BFX3; -.
DR jPOST; Q8BFX3; -.
DR MaxQB; Q8BFX3; -.
DR PaxDb; Q8BFX3; -.
DR PeptideAtlas; Q8BFX3; -.
DR PRIDE; Q8BFX3; -.
DR ProteomicsDB; 268970; -. [Q8BFX3-1]
DR ProteomicsDB; 268971; -. [Q8BFX3-2]
DR Antibodypedia; 2795; 118 antibodies from 22 providers.
DR DNASU; 226823; -.
DR Ensembl; ENSMUST00000085678; ENSMUSP00000082821; ENSMUSG00000026608. [Q8BFX3-1]
DR GeneID; 226823; -.
DR KEGG; mmu:226823; -.
DR UCSC; uc007eai.1; mouse. [Q8BFX3-1]
DR CTD; 51133; -.
DR MGI; MGI:2444629; Kctd3.
DR VEuPathDB; HostDB:ENSMUSG00000026608; -.
DR eggNOG; KOG2714; Eukaryota.
DR GeneTree; ENSGT00940000153881; -.
DR HOGENOM; CLU_012214_1_0_1; -.
DR InParanoid; Q8BFX3; -.
DR OMA; YFMHSAS; -.
DR OrthoDB; 351369at2759; -.
DR PhylomeDB; Q8BFX3; -.
DR TreeFam; TF313754; -.
DR Reactome; R-MMU-9013148; CDC42 GTPase cycle.
DR BioGRID-ORCS; 226823; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Kctd3; mouse.
DR PRO; PR:Q8BFX3; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q8BFX3; protein.
DR Bgee; ENSMUSG00000026608; Expressed in manus and 220 other tissues.
DR ExpressionAtlas; Q8BFX3; baseline and differential.
DR Genevisible; Q8BFX3; MM.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0051260; P:protein homooligomerization; IEA:InterPro.
DR Gene3D; 2.130.10.10; -; 2.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR InterPro; IPR003131; T1-type_BTB.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF02214; BTB_2; 1.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00320; WD40; 3.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Membrane; Phosphoprotein;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..815
FT /note="BTB/POZ domain-containing protein KCTD3"
FT /id="PRO_0000247840"
FT DOMAIN 18..87
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT REPEAT 225..272
FT /note="WD 1"
FT REPEAT 277..314
FT /note="WD 2"
FT REPEAT 316..351
FT /note="WD 3"
FT REPEAT 420..458
FT /note="WD 4"
FT REPEAT 538..578
FT /note="WD 5"
FT REGION 513..815
FT /note="Interaction with HCN3"
FT /evidence="ECO:0000269|PubMed:23382386"
FT REGION 707..815
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 714..763
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 773..799
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 605
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y597"
FT MOD_RES 665
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y597"
FT MOD_RES 712
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y597"
FT MOD_RES 793
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y597"
FT VAR_SEQ 382..403
FT /note="VSGNWIEIAYGTSSGAVRVIVQ -> QYTVSAETGLRSPMVRALEPCE (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_020070"
FT VAR_SEQ 404..815
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_020071"
SQ SEQUENCE 815 AA; 88862 MW; E2A45B4169A6E40C CRC64;
MAGGHCGSFA ASAASSGEIV QLNVGGTRFS TSRQTLMWIP DSFFSSLLSG RISTLRDETG
AIFIDRDPAA FAPILNFLRT KELDLRGVSI NVLRHEAEFY GITPLVRRLL LCEELERSSC
GSVLFHGYLP PPGIPSRKVT NTARSSVDAR NGLNSMEGEA RGNGTQPVLA GNGEDTIRLG
VPVDPRKVLI VAGHHNWIVA AYAHFAVCYR IKESSGWQQV FTSPYLDWTI ERVALNAKVV
GGPHGDKDKM VAVASESSII LWSVQDGGSG SEIGVFSLGV PVDALFFIGN QLVATSHTGK
VGVWNAVTQH WQVQDVVPIT SYDTAGSFLL LGCNNGSIYY IDMQKFPLRM KDNDLLVTEL
YHDPSNDAVT ALSVYLTPKT SVSGNWIEIA YGTSSGAVRV IVQHPETVGS GPQLFQTFTV
HRSPVTKIML SEKHLVSVCA DNNHVRTWTV TRFRGMISTQ PGSTPLASFK ILSLEETESH
GSYSSGNDIG PFGERDDQQV FIQKVVPITN KLFVRLSSTG KRICEIQAVD CTTISSFTVR
ECEGSSRMGS RPRRYLFTGH TNGSIQMWDL TTAMDMVNKT EDKDAGGPTE EELLKLLDQC
DLSTSRCATP NISPATSVLQ HSRLRESSSS LQLQHHETIH EAATYGATRP YRESPLLARA
RRTESFHSYR DFQTLNLNRS IERPVPENGH MGPAQTEVKG AAGECAMSER RSPGTEVRCG
RESDGGLEVH RTAEGLSEPK KRSAEDENEH KVEFRKKGFE AGGFLGRKKV PYLASSPSTS
DGGTDSPGTA SPSPTKTTPS PRHKKSDSSG QEYSL
