ID   5057R_ASFB7             Reviewed;         528 AA.
AC   Q89925;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Protein MGF 505-7R;
GN   OrderedLocusNames=BA71R-029; ORFNames=A528R;
OS   African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10498;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA   Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA   Rodriguez J.F., Vinuela E.;
RT   "Analysis of the complete nucleotide sequence of African swine fever
RT   virus.";
RL   Virology 208:249-278(1995).
RN   [2]
RP   FUNCTION, INTERACTION WITH HOST STING1, AND SUBCELLULAR LOCATION.
RX   PubMed=33712518; DOI=10.4049/jimmunol.2001110;
RA   Li D., Yang W., Li L., Li P., Ma Z., Zhang J., Qi X., Ren J., Ru Y.,
RA   Niu Q., Liu Z., Liu X., Zheng H.;
RT   "African Swine Fever Virus MGF-505-7R Negatively Regulates cGAS-STING-
RT   Mediated Signaling Pathway.";
RL   J. Immunol. 206:1844-1857(2021).
RN   [3]
RP   FUNCTION, INTERACTION WITH HOST JAK1 AND JAK2, AND INDUCTION.
RX   PubMed=34517008; DOI=10.1016/j.jbc.2021.101190;
RA   Li D., Zhang J., Yang W., Li P., Ru Y., Kang W., Li L., Ran Y., Zheng H.;
RT   "African swine fever virus protein MGF-505-7R promotes virulence and
RT   pathogenesis by inhibiting JAK1- and JAK2-mediated signaling.";
RL   J. Biol. Chem. 297:101190-101190(2021).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH HOST RELA.
RX   PubMed=34696476; DOI=10.3390/v13102046;
RA   Liu X., Ao D., Jiang S., Xia N., Xu Y., Shao Q., Luo J., Wang H., Zheng W.,
RA   Chen N., Meurens F., Zhu J.;
RT   "African Swine Fever Virus A528R Inhibits TLR8 Mediated NF-kappaB Activity
RT   by Targeting p65 Activation and Nuclear Translocation.";
RL   Viruses 13:0-0(2021).
CC   -!- FUNCTION: Plays a role in virus cell tropism, and may be required for
CC       efficient virus replication in macrophages. Interferes with host NF-
CC       kappa-B promoter activity mediated by TLR8. Mechanistically, inhibits
CC       the phosphorylation and subsequent nuclear translocation of host NF-
CC       kappa-B RELA subunit downstream of TLR8 (PubMed:34696476). Promotes the
CC       expression of the autophagy-related protein host ULK1 to degrade host
CC       STING and inhibit the interferon response (PubMed:33712518). Inhibits
CC       also JAK1- and JAK2-mediated signaling and thus negatively regulates
CC       the IFN-gamma signaling (PubMed:34517008, PubMed:34696476,
CC       PubMed:33712518). {ECO:0000269|PubMed:33712518,
CC       ECO:0000269|PubMed:34517008, ECO:0000269|PubMed:34696476}.
CC   -!- SUBUNIT: Interacts with host STING1 (PubMed:33712518). Interacts with
CC       host JAK1; this interaction leads to JAK1 degradation
CC       (PubMed:34517008). Interacts with host JAK2; this interaction leads to
CC       JAK2 degradation (PubMed:34517008). Interacts with host RELA; this
CC       interaction inhibits NF-kappa-B promoter activity (PubMed:34696476).
CC       {ECO:0000269|PubMed:33712518, ECO:0000269|PubMed:34517008,
CC       ECO:0000269|PubMed:34696476}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000269|PubMed:34696476}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:34517008}.
CC   -!- SIMILARITY: Belongs to the asfivirus MGF 505 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U02468; AAA17789.1; -; Genomic_DNA.
DR   EMBL; U18466; AAA65260.1; -; Genomic_DNA.
DR   RefSeq; NP_042724.1; NC_001659.2.
DR   SMR; Q89925; -.
DR   GeneID; 22220412; -.
DR   KEGG; vg:22220412; -.
DR   Proteomes; UP000000624; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   Gene3D; 1.25.40.20; -; 1.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   InterPro; IPR004858; MGF_505.
DR   Pfam; PF03158; DUF249; 1.
PE   1: Evidence at protein level;
KW   ANK repeat; Host cytoplasm; Reference proteome; Repeat.
FT   CHAIN           1..528
FT                   /note="Protein MGF 505-7R"
FT                   /id="PRO_0000373339"
FT   REPEAT          54..83
FT                   /note="ANK 1"
FT   REPEAT          129..158
FT                   /note="ANK 2"
FT   REPEAT          261..290
FT                   /note="ANK 3"
FT   REPEAT          292..321
FT                   /note="ANK 4"
FT   REPEAT          322..352
FT                   /note="ANK 5"
SQ   SEQUENCE   528 AA;  61841 MW;  DA9F9BC40C24399F CRC64;
     MFSLQDLCRK NTFFLPNDFS KHTLQRLGLY WKEHGSVHRI EKDSIMIQNE LVLSINDALQ
     LAGEEGDTDV VQLLLLWEGN LHYAIIGALK TENYNLVCEY HSQIQDWHIL LPLIQDPETF
     EKCHDLSLGC DLICLLQHAV KCDMLSILVK YKEDLLNVRI RHRTQSLFVL ACENRRFEII
     EWIGQNLSIP EPEAIFSIAI VTKDVELFSL GYKIIFDYMQ RQGIFQLTNV VRMLLLNRHI
     GMAIEKGLLP FILETLKYGG SVKRALSYAV IDNKRKIIDY LVRHENIPRG TIERLLHLAV
     KKQSSRKTLN LLLSYINYKV KNVKKLLEHV VKYNSTLVIR ILLEKKKNLL DATLTRYVKD
     STYFQVKEFM QDFSISPEKF IKIAVREKRN VLIKGISEDI WENPAERIRN LKQIVCTIKY
     ESGRQFLINI IHTIYQSYSL KPEEILKLAT FYVKHNATTH FKDLCKYLWL NRGTESKKLF
     LECLEIADEK EFPDIKSIVS EYINYLFTAG AITKEEIMQV YALEYAMY