ID   5057R_ASFK5             Reviewed;         528 AA.
AC   P0C9U2;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 18.
DE   RecName: Full=Protein MGF 505-7R;
GN   OrderedLocusNames=Ken-043;
OS   African swine fever virus (isolate Pig/Kenya/KEN-50/1950) (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=561445;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH   NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH   NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Kutish G.F., Rock D.L.;
RT   "African swine fever virus genomes.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a role in virus cell tropism, and may be required for
CC       efficient virus replication in macrophages. Interferes with host NF-
CC       kappa-B promoter activity mediated by TLR8. Mechanistically, inhibits
CC       the phosphorylation and subsequent nuclear translocation of host NF-
CC       kappa-B RELA subunit downstream of TLR8. Promotes the expression of the
CC       autophagy-related protein host ULK1 to degrade host STING and inhibit
CC       the interferon response. Inhibits also JAK1- and JAK2-mediated
CC       signaling and thus negatively regulates the IFN-gamma signaling.
CC       {ECO:0000250|UniProtKB:Q89925}.
CC   -!- SUBUNIT: Interacts with host STING1. Interacts with host JAK1; this
CC       interaction leads to JAK1 degradation. Interacts with host JAK2; this
CC       interaction leads to JAK2 degradation. Interacts with host RELA; this
CC       interaction inhibits NF-kappa-B promoter activity.
CC       {ECO:0000250|UniProtKB:Q89925}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250|UniProtKB:Q89925}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the asfivirus MGF 505 family. {ECO:0000305}.
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DR   EMBL; AY261360; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   SMR; P0C9U2; -.
DR   Proteomes; UP000000861; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR004858; MGF_505.
DR   Pfam; PF03158; DUF249; 1.
PE   3: Inferred from homology;
KW   Early protein; Host cytoplasm.
FT   CHAIN           1..528
FT                   /note="Protein MGF 505-7R"
FT                   /id="PRO_0000373340"
SQ   SEQUENCE   528 AA;  61571 MW;  DE574FDB79AFFFEE CRC64;
     MFSLQDLCRK NTFFLPNDFK KHTLQLLGLY WKEHGSVHRA EKDNIMIQNE LILTVNDALQ
     LAGEEGDTDV VQLLLLWEGN LHYAIIGALK AENYNLICEY HSQIEDWHAL LPLIQDPETF
     EKCHELSLGC DLLCLLQHAI KCNMLSILVK YKEDLLNARI RHRIQSLFVL ACETRRIEII
     LWIGQNLPIP EPETIFSIAV ITKDLELFSL GYKIIFDYMQ RQGIFQLSEV VRKILLNRHI
     DMAVNKGLLP FVLETFKYGG SVKRALSCAV MDNKRKVIDY LVRHENISHQ TIERLLYLAV
     KKHSSRKTLN LLLSYINYKV KNIKKLLDHV LGGNSTLVLK ILLEKKQNLV DAALTRLVKH
     STYFRVKEFI EDFSISPEKF IKIAVREQKN VIIKVICEDI WENPAERIRY LKQIVSSIKY
     ESGRQFLINI IHTIYQSHSL KPEEIFKLAT FYVKHNAVTH FKDLCKFLWL NRGTESKKHF
     LKCLEIADEK DFPAIKSIVS EYINYMFTAG TMTKDEIMQA YASEYTMC