ID   5057R_ASFM2             Reviewed;         528 AA.
AC   P0C9U3;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 30.
DE   RecName: Full=Protein MGF 505-7R;
GN   OrderedLocusNames=Mal-039;
OS   African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10500;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH   NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH   NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Kutish G.F., Rock D.L.;
RT   "African swine fever virus genomes.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a role in virus cell tropism, and may be required for
CC       efficient virus replication in macrophages. Interferes with host NF-
CC       kappa-B promoter activity mediated by TLR8. Mechanistically, inhibits
CC       the phosphorylation and subsequent nuclear translocation of host NF-
CC       kappa-B RELA subunit downstream of TLR8. Promotes the expression of the
CC       autophagy-related protein host ULK1 to degrade host STING and inhibit
CC       the interferon response. Inhibits also JAK1- and JAK2-mediated
CC       signaling and thus negatively regulates the IFN-gamma signaling.
CC       {ECO:0000250|UniProtKB:Q89925}.
CC   -!- SUBUNIT: Interacts with host STING1. Interacts with host JAK1; this
CC       interaction leads to JAK1 degradation. Interacts with host JAK2; this
CC       interaction leads to JAK2 degradation. Interacts with host RELA; this
CC       interaction inhibits NF-kappa-B promoter activity.
CC       {ECO:0000250|UniProtKB:Q89925}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250|UniProtKB:Q89925}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000250|UniProtKB:Q89925}.
CC   -!- SIMILARITY: Belongs to the asfivirus MGF 505 family. {ECO:0000305}.
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DR   EMBL; AY261361; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   SMR; P0C9U3; -.
DR   Proteomes; UP000000860; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0039576; P:suppression by virus of host JAK-STAT cascade via inhibition of JAK1 activity; IEA:UniProtKB-KW.
DR   GO; GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.40.20; -; 1.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   InterPro; IPR004858; MGF_505.
DR   Pfam; PF03158; DUF249; 1.
DR   SUPFAM; SSF48403; SSF48403; 1.
PE   3: Inferred from homology;
KW   ANK repeat; Early protein; Host cytoplasm; Host-virus interaction;
KW   Inhibition of host innate immune response by virus;
KW   Inhibition of host interferon signaling pathway by virus;
KW   Inhibition of host JAK1 by virus; Repeat; Viral immunoevasion.
FT   CHAIN           1..528
FT                   /note="Protein MGF 505-7R"
FT                   /id="PRO_0000373341"
FT   REPEAT          54..83
FT                   /note="ANK 1"
FT   REPEAT          261..291
FT                   /note="ANK 2"
SQ   SEQUENCE   528 AA;  61838 MW;  B806D2C74D5992A5 CRC64;
     MFSLQDLCRK NTFFLPNGFN KHTLQLLGLY WKEHGSVHRV EKDNIMIQNE LVLSINDALL
     LAGEEGDTDV VQLLLLWEGN LHYAIIGALK TEKYNLICEY HSQIEDWHIL LPMIQDPETF
     EKCHDLSLQC DFICLLQHAI KYNKLSILVK YKEDLLNIRI RHRVQSLFVL ACENRRTEII
     EWIGQNLSIP EPDAIFSIAI ATKDLELFSL GYKIIFDYMQ RQGILQLSNG LRMVVLNRHI
     SMAIDNGLLP FVLETLKHGG NIHRALSYAV SHNRRKILDY LIRQKNIPPN TIERLLYLAV
     KNQSSRKTLN LLLSYINYKV KNVKKLVEHV VDHNSTLVVT ILLEKKINLI DAVLTRLVKH
     STYFRVREFI QEFSISPEKF IKIAVREKRN VIIKGISEDI WENPAERITY LKQIVHTIKY
     ESGREFLINI IHTIYQSYSL KPEEILKLAR FYVKHNATTH FKDLCKYLWL NRGTESKKLF
     LECLEIADKK DFPAIKNIVS EYINYLFTAG AITKEEIMQV YTLEYAMY