位置:首页 > 蛋白库 > AQP4_DIPME
AQP4_DIPME
ID   AQP4_DIPME              Reviewed;         324 AA.
AC   Q923J4; Q923J5;
DT   10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Aquaporin-4;
DE            Short=AQP-4;
GN   Name=AQP4;
OS   Dipodomys merriami (Merriam's kangaroo rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Castorimorpha; Heteromyidae;
OC   Dipodomyinae; Dipodomys.
OX   NCBI_TaxID=94247;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Fang P.K., Huang Y., Walsberg G.E., Brown D., van Hoek A.N.;
RT   "Molecular cloning and characterization of Merriam's kangaroo rat aquaporin
RT   4.";
RL   Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   TISSUE SPECIFICITY.
RX   PubMed=11292621; DOI=10.1152/ajprenal.2001.280.5.f794;
RA   Huang Y., Tracy R., Walsberg G.E., Makkinje A., Fang P., Brown D.,
RA   Van Hoek A.N.;
RT   "Absence of aquaporin-4 water channels from kidneys of the desert rodent
RT   Dipodomys merriami merriami.";
RL   Am. J. Physiol. 280:F794-F802(2001).
CC   -!- FUNCTION: Forms a water-specific channel. Plays an important role in
CC       brain water homeostasis and in glymphatic solute transport. Required
CC       for a normal rate of water exchange across the blood brain interface.
CC       Required for normal levels of cerebrospinal fluid influx into the brain
CC       cortex and parenchyma along paravascular spaces that surround
CC       penetrating arteries, and for normal drainage of interstitial fluid
CC       along paravenous drainage pathways. Thereby, it is required for normal
CC       clearance of solutes from the brain interstitial fluid, including
CC       soluble beta-amyloid peptides derived from APP. Plays a redundant role
CC       in urinary water homeostasis and urinary concentrating ability.
CC       {ECO:0000250|UniProtKB:P55088}.
CC   -!- SUBUNIT: Homotetramer. The tetramers can form oligomeric arrays in
CC       membranes. The size of the oligomers differs between tissues and is
CC       smaller in skeletal muscle than in brain. Interaction between AQP4
CC       oligomeric arrays in close-by cells can contribute to cell-cell
CC       adhesion (By similarity). Part of a complex containing MLC1, TRPV4,
CC       HEPACAM and ATP1B1 (By similarity). {ECO:0000250|UniProtKB:P47863,
CC       ECO:0000250|UniProtKB:P55087}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P55088};
CC       Multi-pass membrane protein {ECO:0000250|UniProtKB:P55087}. Basolateral
CC       cell membrane {ECO:0000250|UniProtKB:P55088}; Multi-pass membrane
CC       protein {ECO:0000250|UniProtKB:P55087}. Endosome membrane
CC       {ECO:0000250|UniProtKB:P47863}. Cell membrane, sarcolemma
CC       {ECO:0000250|UniProtKB:P55088}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:P55087}. Cell projection
CC       {ECO:0000250|UniProtKB:P55088}. Note=Activation of the vasopressin
CC       receptor AVPR1A triggers AQP4 phosphorylation at Ser-180 and promotes
CC       its internalization from the cell membrane (By similarity). Detected on
CC       brain astrocyte processes and astrocyte endfeet close to capillaries
CC       (By similarity). {ECO:0000250|UniProtKB:P47863,
CC       ECO:0000250|UniProtKB:P55088}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=2;
CC         IsoId=Q923J4-1; Sequence=Displayed;
CC       Name=1;
CC         IsoId=Q923J4-2; Sequence=VSP_010210;
CC   -!- TISSUE SPECIFICITY: Not expressed in kidney, Detectable in gastric
CC       parietal and brain astroglial cells. The absence of AQP4 in kidney may
CC       be critical for the extreme urinary concentration that occurs in this
CC       species (up to 5,000 mosmol/kg H(2)O). {ECO:0000269|PubMed:11292621}.
CC   -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC       membrane-spanning domains and a pore-forming loop with the signature
CC       motif Asn-Pro-Ala (NPA). {ECO:0000250|UniProtKB:P55087}.
CC   -!- PTM: Phosphorylation by PKC at Ser-180 reduces conductance by 50%.
CC       Phosphorylation by PKG at Ser-111 in response to glutamate increases
CC       conductance by 40% (By similarity). {ECO:0000250}.
CC   -!- PTM: Isoform 2: Palmitoylated on its N-terminal region. Isoform 1: Not
CC       palmitoylated. {ECO:0000250|UniProtKB:P47863}.
CC   -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY032857; AAK66823.1; -; mRNA.
DR   EMBL; AY032858; AAK66824.1; -; mRNA.
DR   AlphaFoldDB; Q923J4; -.
DR   SMR; Q923J4; -.
DR   GO; GO:0097450; C:astrocyte end-foot; ISS:UniProtKB.
DR   GO; GO:0016323; C:basolateral plasma membrane; ISS:UniProtKB.
DR   GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; IEA:GOC.
DR   GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR   GO; GO:0042383; C:sarcolemma; ISS:UniProtKB.
DR   GO; GO:0015250; F:water channel activity; ISS:UniProtKB.
DR   GO; GO:0009992; P:cellular water homeostasis; ISS:UniProtKB.
DR   GO; GO:0090660; P:cerebrospinal fluid circulation; ISS:UniProtKB.
DR   GO; GO:0051289; P:protein homotetramerization; ISS:UniProtKB.
DR   GO; GO:0030104; P:water homeostasis; ISS:UniProtKB.
DR   GO; GO:0006833; P:water transport; ISS:UniProtKB.
DR   CDD; cd00333; MIP; 1.
DR   Gene3D; 1.20.1080.10; -; 1.
DR   InterPro; IPR023271; Aquaporin-like.
DR   InterPro; IPR034294; Aquaporin_transptr.
DR   InterPro; IPR000425; MIP.
DR   InterPro; IPR022357; MIP_CS.
DR   PANTHER; PTHR19139; PTHR19139; 1.
DR   Pfam; PF00230; MIP; 1.
DR   PRINTS; PR00783; MINTRINSICP.
DR   SUPFAM; SSF81338; SSF81338; 1.
DR   TIGRFAMs; TIGR00861; MIP; 1.
DR   PROSITE; PS00221; MIP; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cell membrane; Cell projection; Endosome;
KW   Glycoprotein; Lipoprotein; Membrane; Palmitate; Phosphoprotein; Repeat;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..324
FT                   /note="Aquaporin-4"
FT                   /id="PRO_0000063947"
FT   TOPO_DOM        1..36
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        37..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P55087"
FT   TOPO_DOM        58..69
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        70..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P55087"
FT   TOPO_DOM        90..93
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   INTRAMEM        94..101
FT                   /note="Discontinuously helical"
FT                   /evidence="ECO:0000250|UniProtKB:P55087"
FT   TOPO_DOM        102..115
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        116..136
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P55087"
FT   TOPO_DOM        137..155
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        156..176
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P55087"
FT   TOPO_DOM        177..184
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        185..205
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P55087"
FT   TOPO_DOM        206..208
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   INTRAMEM        209..222
FT                   /note="Discontinuously helical"
FT                   /evidence="ECO:0000250|UniProtKB:P55087"
FT   TOPO_DOM        223..231
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        232..252
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P55087"
FT   TOPO_DOM        253..324
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   REGION          305..324
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           97..99
FT                   /note="NPA 1"
FT                   /evidence="ECO:0000250|UniProtKB:P55087"
FT   MOTIF           213..215
FT                   /note="NPA 2"
FT                   /evidence="ECO:0000250|UniProtKB:P55087"
FT   MOD_RES         111
FT                   /note="Phosphoserine; by PKG"
FT                   /evidence="ECO:0000250|UniProtKB:P55088"
FT   MOD_RES         180
FT                   /note="Phosphoserine; by PKC"
FT                   /evidence="ECO:0000250|UniProtKB:P47863"
FT   MOD_RES         276
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P47863"
FT   MOD_RES         285
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P55088"
FT   MOD_RES         289
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:P55088"
FT   LIPID           13
FT                   /note="S-palmitoyl cysteine"
FT                   /evidence="ECO:0000250|UniProtKB:P47863"
FT   LIPID           17
FT                   /note="S-palmitoyl cysteine"
FT                   /evidence="ECO:0000250|UniProtKB:P47863"
FT   CARBOHYD        153
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        206
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         1..22
FT                   /note="Missing (in isoform 1)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_010210"
SQ   SEQUENCE   324 AA;  34940 MW;  EAE9CE3B17952CDD CRC64;
     MSDRPAARPW GKCGSLCRRE EIMVAFKGVW TQAFWKAVTA EFLAMLIFVL LSLGSTINWG
     GKENPLPVDM VLISLCFGLS IATMVQCFGH ISGGHINPAV TVAMVCTRKI SIAKSVFYIA
     AQCLGAIIGA GILYLVTPPS VVGGLGVTTV HGNLTAGHGL LVELIITFQL VFTIFASCDS
     KRTDVTGSIA LAIGFSVAIG HLFAINYTGA SMNPARSFGP AVIMGNWENH WIYWVGPIIG
     AVLAGGLYEY VFCPDVELKR RFKEAFSKAA QQTKGSYMEV EDNRSQVETE DLILKPGLVH
     VIDIDRGDEK KGKDPSGEIA QTQH
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024