ID   KDGD_METRJ              Reviewed;         304 AA.
AC   B1LW44;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Probable 5-dehydro-4-deoxyglucarate dehydratase {ECO:0000255|HAMAP-Rule:MF_00694};
DE            EC=4.2.1.41 {ECO:0000255|HAMAP-Rule:MF_00694};
DE   AltName: Full=5-keto-4-deoxy-glucarate dehydratase {ECO:0000255|HAMAP-Rule:MF_00694};
DE            Short=KDGDH {ECO:0000255|HAMAP-Rule:MF_00694};
GN   OrderedLocusNames=Mrad2831_5150;
OS   Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 /
OS   NBRC 15690 / NCIMB 10815 / 0-1).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylobacterium.
OX   NCBI_TaxID=426355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C.,
RA   Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Marx C.J., Richardson P.;
RT   "Complete sequence of chromosome of Methylobacterium radiotolerans JCM
RT   2831.";
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-dehydro-4-deoxy-D-glucarate + H(+) = 2,5-dioxopentanoate +
CC         CO2 + H2O; Xref=Rhea:RHEA:24608, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:42819,
CC         ChEBI:CHEBI:58136; EC=4.2.1.41; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00694};
CC   -!- PATHWAY: Carbohydrate acid metabolism; D-glucarate degradation; 2,5-
CC       dioxopentanoate from D-glucarate: step 2/2. {ECO:0000255|HAMAP-
CC       Rule:MF_00694}.
CC   -!- SIMILARITY: Belongs to the DapA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00694}.
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DR   EMBL; CP001001; ACB27107.1; -; Genomic_DNA.
DR   RefSeq; WP_012322051.1; NC_010505.1.
DR   AlphaFoldDB; B1LW44; -.
DR   SMR; B1LW44; -.
DR   STRING; 426355.Mrad2831_5150; -.
DR   EnsemblBacteria; ACB27107; ACB27107; Mrad2831_5150.
DR   KEGG; mrd:Mrad2831_5150; -.
DR   PATRIC; fig|426355.14.peg.5224; -.
DR   eggNOG; COG0329; Bacteria.
DR   HOGENOM; CLU_049343_5_2_5; -.
DR   OMA; EFHALTP; -.
DR   OrthoDB; 1438588at2; -.
DR   UniPathway; UPA00564; UER00628.
DR   Proteomes; UP000006589; Chromosome.
DR   GO; GO:0047448; F:5-dehydro-4-deoxyglucarate dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0042838; P:D-glucarate catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00951; KDGDH; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   HAMAP; MF_00694; KDGDH; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR002220; DapA-like.
DR   InterPro; IPR017655; Dehydro-deoxyglucarate_dehyd.
DR   PANTHER; PTHR12128; PTHR12128; 1.
DR   Pfam; PF00701; DHDPS; 1.
DR   PIRSF; PIRSF001365; DHDPS; 1.
DR   SMART; SM01130; DHDPS; 1.
DR   TIGRFAMs; TIGR03249; KdgD; 1.
PE   3: Inferred from homology;
KW   Lyase; Reference proteome.
FT   CHAIN           1..304
FT                   /note="Probable 5-dehydro-4-deoxyglucarate dehydratase"
FT                   /id="PRO_1000132271"
SQ   SEQUENCE   304 AA;  32809 MW;  DDCC95B06A27AF1B CRC64;
     MASMTPAEMA RVLGSGLLSF PVTHFRDDLS FDETAYRDNL GRLADYKVSG LFAAGGTGEF
     FSLTPAEIDR VLRAAVEETR GRTPVIAPAG QGTALAVEMA RAAEAAGADG ILLLPPYLVG
     SEQAGLAAHI EAVCRATALG IIVYNRANAQ LDERTLAGLC ERCPNLVGFK DGVGDVELMT
     RVYAALGDRL TYIGGLPTAE TFALPYLEMG VTTYSSAIFN FLPEWALSFY DSVRRRDRDA
     VYRELRDFVL PYIAIRNRKR GYAVSIVKAG MSAVGRHAGP VRPPLTELDA AERAELTALI
     GDRR