AQP8_MOUSE
ID AQP8_MOUSE Reviewed; 261 AA.
AC P56404; Q91XC2;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Aquaporin-8;
DE Short=AQP-8;
GN Name=Aqp8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=9388476; DOI=10.1006/bbrc.1997.7664;
RA Ma T., Yang B., Verkman A.S.;
RT "Cloning of a novel water and urea-permeable aquaporin from mouse expressed
RT strongly in colon, placenta, liver, and heart.";
RL Biochem. Biophys. Res. Commun. 240:324-328(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129;
RX PubMed=10449905; DOI=10.1159/000015300;
RA Calamita G., Spalluto C., Mazzone A., Rocchi M., Svelto M.;
RT "Cloning, structural organization and chromosomal localization of the mouse
RT aquaporin-8 water channel gene (Aqp8).";
RL Cytogenet. Cell Genet. 85:237-241(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Forms a water-specific channel; mercury-sensitive. Also
CC permeable to urea but not to glycerol.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Colon, liver, heart and placenta.
CC -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC membrane-spanning domains and a pore-forming loop with the signature
CC motif Asn-Pro-Ala (NPA).
CC -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
CC {ECO:0000305}.
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DR EMBL; AF018952; AAB68847.1; -; mRNA.
DR EMBL; AF107543; AAD55972.1; -; Genomic_DNA.
DR EMBL; AF107538; AAD55972.1; JOINED; Genomic_DNA.
DR EMBL; AF107539; AAD55972.1; JOINED; Genomic_DNA.
DR EMBL; AF107540; AAD55972.1; JOINED; Genomic_DNA.
DR EMBL; AF107541; AAD55972.1; JOINED; Genomic_DNA.
DR EMBL; AF107542; AAD55972.1; JOINED; Genomic_DNA.
DR EMBL; BC010982; AAH10982.1; -; mRNA.
DR CCDS; CCDS72038.1; -.
DR PIR; JC5806; JC5806.
DR RefSeq; NP_001102515.1; NM_001109045.1.
DR RefSeq; NP_031500.1; NM_007474.2.
DR AlphaFoldDB; P56404; -.
DR SMR; P56404; -.
DR STRING; 10090.ENSMUSP00000033023; -.
DR GlyGen; P56404; 1 site.
DR PhosphoSitePlus; P56404; -.
DR MaxQB; P56404; -.
DR PaxDb; P56404; -.
DR PRIDE; P56404; -.
DR Antibodypedia; 26204; 198 antibodies from 31 providers.
DR DNASU; 11833; -.
DR Ensembl; ENSMUST00000098056; ENSMUSP00000095664; ENSMUSG00000030762.
DR GeneID; 11833; -.
DR KEGG; mmu:11833; -.
DR UCSC; uc012ftk.2; mouse.
DR CTD; 343; -.
DR MGI; MGI:1195271; Aqp8.
DR VEuPathDB; HostDB:ENSMUSG00000030762; -.
DR eggNOG; KOG0223; Eukaryota.
DR GeneTree; ENSGT00940000159304; -.
DR HOGENOM; CLU_020019_3_5_1; -.
DR InParanoid; P56404; -.
DR OMA; GTMQANV; -.
DR OrthoDB; 1152704at2759; -.
DR PhylomeDB; P56404; -.
DR TreeFam; TF312940; -.
DR Reactome; R-MMU-432047; Passive transport by Aquaporins.
DR BioGRID-ORCS; 11833; 0 hits in 67 CRISPR screens.
DR PRO; PR:P56404; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; P56404; protein.
DR Bgee; ENSMUSG00000030762; Expressed in left colon and 51 other tissues.
DR ExpressionAtlas; P56404; baseline and differential.
DR Genevisible; P56404; MM.
DR GO; GO:0045177; C:apical part of cell; ISO:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; TAS:MGI.
DR GO; GO:0046691; C:intracellular canaliculus; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0015250; F:water channel activity; ISO:MGI.
DR GO; GO:0015722; P:canalicular bile acid transport; ISO:MGI.
DR GO; GO:0071320; P:cellular response to cAMP; IEA:Ensembl.
DR GO; GO:0006833; P:water transport; IMP:MGI.
DR Gene3D; 1.20.1080.10; -; 1.
DR InterPro; IPR023271; Aquaporin-like.
DR InterPro; IPR023277; Aquaporin_8.
DR InterPro; IPR034294; Aquaporin_transptr.
DR InterPro; IPR000425; MIP.
DR InterPro; IPR022357; MIP_CS.
DR PANTHER; PTHR45665; PTHR45665; 1.
DR Pfam; PF00230; MIP; 1.
DR PRINTS; PR02020; AQUAPORIN8.
DR PRINTS; PR00783; MINTRINSICP.
DR SUPFAM; SSF81338; SSF81338; 1.
DR PROSITE; PS00221; MIP; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..261
FT /note="Aquaporin-8"
FT /id="PRO_0000063962"
FT TOPO_DOM 1..36
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 37..57
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 58..84
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 106..107
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 129..156
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 157..177
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 178..183
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 205..228
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 250..261
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOTIF 92..94
FT /note="NPA 1"
FT MOTIF 210..212
FT /note="NPA 2"
FT CARBOHYD 139
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 5
FT /note="Missing (in Ref. 3; AAH10982)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 261 AA; 27797 MW; 9CB48ACA1FB5E589 CRC64;
MSGEQTPMCS MDLPEVKVKT SMAGRCRVFW YEQYVQPCIV ELVGSALFIF IGCLSVIENS
PNTGLLQPAL AHGLALGLII ATLGNISGGH FNPAVSLAVT VIGGLKTMLL IPYWISQLFG
GLIGAALAKV VSPEERFWNA SGAAFAIVQE QEQVAEALGI EIILTMLLVL AVCMGAVNEK
TMGPLAPFSI GFSVIVDILA GGSISGACMN PARAFGPAVM AGYWDFHWIY WLGPLLAGLF
VGLLIRLLIG DEKTRLILKS R
