AQPA_DICDI
ID AQPA_DICDI Reviewed; 279 AA.
AC Q9U8P7; Q55FK7;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Aquaporin A;
GN Name=aqpA; ORFNames=DDB_G0267378;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, FUNCTION, AND
RP INDUCTION.
RC STRAIN=AX4;
RX PubMed=10876090; DOI=10.1016/s0378-1119(00)00201-8;
RA Mitra B.N., Yoshino R., Morio T., Yokoyama M., Maeda M., Urushihara H.,
RA Tanaka Y.;
RT "Loss of a member of the aquaporin gene family, aqpA affects spore dormancy
RT in Dictyostelium.";
RL Gene 251:131-139(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=AX4;
RA Mitra B.N., Yoshino R., Morio T., Urushihara H., Tanaka Y.;
RT "Characterization of a water channel protein gene aqpA in Dictyostelium
RT discoideum.";
RL Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: May form a water-specific channel (By similarity). Required
CC for prolonged spore survival on fruiting bodies. {ECO:0000250,
CC ECO:0000269|PubMed:10876090}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: First detected at the tight mound stage. Highly
CC expressed in fingers and late culminants. In slugs, detected in
CC prespore cells, but not in prestalk cells.
CC {ECO:0000269|PubMed:10876090}.
CC -!- INDUCTION: Constitutively expressed during hyperosmotic stress.
CC {ECO:0000269|PubMed:10876090}.
CC -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC membrane-spanning domains and a pore-forming loop with the signature
CC motif Asn-Pro-Ala (NPA). Here, the second motif is Asn-Pro-Val (NPV).
CC -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
CC {ECO:0000305}.
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DR EMBL; AB032841; BAA85158.1; -; Genomic_DNA.
DR EMBL; AB008431; BAF94060.1; -; mRNA.
DR EMBL; AAFI02000003; EAL73141.1; -; Genomic_DNA.
DR RefSeq; XP_647526.1; XM_642434.1.
DR AlphaFoldDB; Q9U8P7; -.
DR SMR; Q9U8P7; -.
DR STRING; 44689.DDB0191271; -.
DR PaxDb; Q9U8P7; -.
DR EnsemblProtists; EAL73141; EAL73141; DDB_G0267378.
DR GeneID; 8616333; -.
DR KEGG; ddi:DDB_G0267378; -.
DR dictyBase; DDB_G0267378; aqpA.
DR eggNOG; KOG0223; Eukaryota.
DR HOGENOM; CLU_020019_3_3_1; -.
DR InParanoid; Q9U8P7; -.
DR OMA; WTPGPLH; -.
DR PhylomeDB; Q9U8P7; -.
DR Reactome; R-DDI-1237044; Erythrocytes take up carbon dioxide and release oxygen.
DR Reactome; R-DDI-1247673; Erythrocytes take up oxygen and release carbon dioxide.
DR Reactome; R-DDI-432040; Vasopressin regulates renal water homeostasis via Aquaporins.
DR Reactome; R-DDI-432047; Passive transport by Aquaporins.
DR PRO; PR:Q9U8P7; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015250; F:water channel activity; IBA:GO_Central.
DR GO; GO:0006833; P:water transport; IBA:GO_Central.
DR CDD; cd00333; MIP; 1.
DR Gene3D; 1.20.1080.10; -; 1.
DR InterPro; IPR023271; Aquaporin-like.
DR InterPro; IPR034294; Aquaporin_transptr.
DR InterPro; IPR000425; MIP.
DR InterPro; IPR022357; MIP_CS.
DR PANTHER; PTHR19139; PTHR19139; 1.
DR Pfam; PF00230; MIP; 1.
DR PRINTS; PR00783; MINTRINSICP.
DR SUPFAM; SSF81338; SSF81338; 1.
DR PROSITE; PS00221; MIP; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..279
FT /note="Aquaporin A"
FT /id="PRO_0000327505"
FT TOPO_DOM 1..40
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 62..69
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 91..114
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 136..158
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 180..188
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 210..227
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 249..279
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOTIF 96..98
FT /note="NPA 1"
FT /evidence="ECO:0000250"
FT MOTIF 214..216
FT /note="NPA 2"
FT /evidence="ECO:0000250"
SQ SEQUENCE 279 AA; 29906 MW; 1B4928E57ACC7EA7 CRC64;
MVKVVPLRFI TYDPLKDPSK MIYRRPISKP VKAFKGFFSE FLGTLYLVYF CGGSVCAAFA
VAGDSAARAL LGGLIQGMAL AALIWAVSGV SGCNLNPAVT LANLLSGRVG LIDSLYYVAA
QILGCIAGAG ILYGCLPNMY RIDLGVPHLA PGMNTGQAFL MEMMLTSILC LCVLGTSVFN
VWDRRLNRIA PFAIGLALFI GVAIGFNFSG GALNPVRVLG PSIISGVWSH HWVYWLGPIV
GAILAAFIYR CLLQERFDVI ERPGYIAPLI DPSTAVSSY
