AQPS_RHIME
ID AQPS_RHIME Reviewed; 233 AA.
AC Q92R43;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Aquaglyceroporin AqpS {ECO:0000303|PubMed:16199569};
GN Name=aqpS {ECO:0000303|PubMed:16199569};
GN ORFNames=SMc02648 {ECO:0000312|EMBL:CAC45655.1};
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=1021;
RX PubMed=16199569; DOI=10.1128/jb.187.20.6991-6997.2005;
RA Yang H.C., Cheng J., Finan T.M., Rosen B.P., Bhattacharjee H.;
RT "Novel pathway for arsenic detoxification in the legume symbiont
RT Sinorhizobium meliloti.";
RL J. Bacteriol. 187:6991-6997(2005).
RN [4]
RP FUNCTION IN TRANSPORT OF METHYLARSENICALS, AND MUTAGENESIS OF THR-49 AND
RP VAL-177.
RC STRAIN=1021;
RX PubMed=33418223; DOI=10.1016/j.chemosphere.2020.129379;
RA Chen J., Nadar V.S., Rosen B.P.;
RT "Aquaglyceroporin AqpS from Sinorhizobium meliloti conducts both trivalent
RT and pentavalent methylarsenicals.";
RL Chemosphere 270:129379-129379(2021).
CC -!- FUNCTION: Involved in resistance to arsenic. Facilitates efflux of
CC arsenite [As(III)]. Arsenate [As(V)] enters the cell through phosphate
CC transport systems and is reduced to arsenite by the arsenate reductase
CC ArsC. Internally generated arsenite flows out of the cell by downhill
CC movement through AqpS (PubMed:16199569). Can also transport the highly
CC toxic methylarsenite [MAs(III)] and the relatively non-toxic
CC methylarsenate [MAs(V)]. May be a component of an methylarsenite
CC resistance pathway in which methylarsenite enters cells via AqpS, is
CC oxidized by ArsH to methylarsenate, which exits the cells via AqpS.
CC This pathway may confer a selective advantage for R.melliloti to grow
CC in the presence of environmental methylarsenicals (PubMed:33418223).
CC {ECO:0000269|PubMed:16199569, ECO:0000269|PubMed:33418223}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC membrane-spanning domains and a pore-forming loop with the signature
CC motif Asn-Pro-Ala (NPA). {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Disruption of the gene results in increased
CC tolerance to arsenite but not arsenate. Does not affect sensitivity to
CC antimonate [Sb(V)]. {ECO:0000269|PubMed:16199569}.
CC -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. NIP (TC
CC 1.A.8.12) subfamily. {ECO:0000305}.
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DR EMBL; AL591688; CAC45655.1; -; Genomic_DNA.
DR RefSeq; NP_385182.1; NC_003047.1.
DR RefSeq; WP_010969025.1; NC_003047.1.
DR AlphaFoldDB; Q92R43; -.
DR SMR; Q92R43; -.
DR STRING; 266834.SMc02648; -.
DR TCDB; 1.A.8.12.10; the major intrinsic protein (mip) family.
DR EnsemblBacteria; CAC45655; CAC45655; SMc02648.
DR GeneID; 61602536; -.
DR KEGG; sme:SMc02648; -.
DR PATRIC; fig|266834.11.peg.2482; -.
DR eggNOG; COG0580; Bacteria.
DR HOGENOM; CLU_020019_8_0_5; -.
DR OMA; TGGAQWF; -.
DR BioCyc; MetaCyc:MON-21671; -.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015267; F:channel activity; IEA:InterPro.
DR GO; GO:0046685; P:response to arsenic-containing substance; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1080.10; -; 1.
DR InterPro; IPR023271; Aquaporin-like.
DR InterPro; IPR034294; Aquaporin_transptr.
DR InterPro; IPR000425; MIP.
DR PANTHER; PTHR45724; PTHR45724; 1.
DR Pfam; PF00230; MIP; 2.
DR PRINTS; PR00783; MINTRINSICP.
DR SUPFAM; SSF81338; SSF81338; 1.
PE 1: Evidence at protein level;
KW Arsenical resistance; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..233
FT /note="Aquaglyceroporin AqpS"
FT /id="PRO_0000453039"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOTIF 69..71
FT /note="NPA 1"
FT /evidence="ECO:0000305"
FT MOTIF 174..176
FT /note="NPA 2"
FT /evidence="ECO:0000305"
FT MUTAGEN 49
FT /note="T->F,W: Increases uptake of both methylarsenite and
FT methylarsenate and exhibits higher sensitivity to
FT methylarsenite."
FT /evidence="ECO:0000269|PubMed:33418223"
FT MUTAGEN 177
FT /note="V->I: Decreases methylarsenite transport activity
FT and results in higher resistance to methylarsenite. Slight
FT decrease in methylarsenate transport activity."
FT /evidence="ECO:0000269|PubMed:33418223"
FT MUTAGEN 177
FT /note="V->R: Increases methylarsenite transport activity,
FT leading to lower resistance to methylarsenite. Nearly
FT complete loss of methylarsenate transport activity."
FT /evidence="ECO:0000269|PubMed:33418223"
SQ SEQUENCE 233 AA; 24301 MW; ABAD5ACF96C95655 CRC64;
MQEFDLTRRC VAEALGTGLL VAAVVGSGIM ADALTADDAL ALVANTIATG AILVVLVTIL
GPLSGAHFNP AVSLVFALSG RLTRRDCAAY VIAQVAGAIA GTALAHLMFD LPPLDMSMKV
RTGPAQWLSE GVAAFGLVAT ILAGIRFHRE AVPWLVGLYI TAAYWFTAST SFANPAVALA
RSFTNTFSGI RPGDLPGFVI AELLGAVCAL ALMRWLLQPA RPIIRQTSPE TAP
