AQP_DROME
ID AQP_DROME Reviewed; 245 AA.
AC Q9V5Z7; Q24148; Q3KN56; Q8MKY6;
DT 03-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Aquaporin;
GN Name=Drip; ORFNames=CG9023;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 82-191.
RC STRAIN=Oregon-R; TISSUE=Malpighian tubule;
RA Dow J.A.T., Kelly D.C., Davies S.A., Maddrell S.H.P., Brown D.;
RT "A member of the major intrinsic protein family in Drosophila tubules.";
RL J. Physiol. (Lond.) 489:110-110(1995).
CC -!- FUNCTION: Forms a water-specific channel. {ECO:0000250}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC membrane-spanning domains and a pore-forming loop with the signature
CC motif Asn-Pro-Ala (NPA).
CC -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
CC {ECO:0000305}.
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DR EMBL; AE013599; AAF58643.2; -; Genomic_DNA.
DR EMBL; AE013599; AAM68740.2; -; Genomic_DNA.
DR EMBL; BT023883; ABA81817.1; -; mRNA.
DR EMBL; U38807; AAA81324.1; -; mRNA.
DR RefSeq; NP_523697.1; NM_078973.3.
DR RefSeq; NP_725051.2; NM_165833.3.
DR AlphaFoldDB; Q9V5Z7; -.
DR SMR; Q9V5Z7; -.
DR BioGRID; 62024; 1.
DR DIP; DIP-23832N; -.
DR IntAct; Q9V5Z7; 17.
DR STRING; 7227.FBpp0303808; -.
DR TCDB; 1.A.8.8.13; the major intrinsic protein (mip) family.
DR PaxDb; Q9V5Z7; -.
DR DNASU; 36236; -.
DR EnsemblMetazoa; FBtr0088141; FBpp0087240; FBgn0015872.
DR EnsemblMetazoa; FBtr0088142; FBpp0087241; FBgn0015872.
DR GeneID; 36236; -.
DR KEGG; dme:Dmel_CG9023; -.
DR CTD; 36236; -.
DR FlyBase; FBgn0015872; Drip.
DR VEuPathDB; VectorBase:FBgn0015872; -.
DR eggNOG; KOG0223; Eukaryota.
DR GeneTree; ENSGT00940000176123; -.
DR InParanoid; Q9V5Z7; -.
DR OrthoDB; 1152704at2759; -.
DR PhylomeDB; Q9V5Z7; -.
DR Reactome; R-DME-1237044; Erythrocytes take up carbon dioxide and release oxygen.
DR Reactome; R-DME-1247673; Erythrocytes take up oxygen and release carbon dioxide.
DR Reactome; R-DME-432040; Vasopressin regulates renal water homeostasis via Aquaporins.
DR Reactome; R-DME-432047; Passive transport by Aquaporins.
DR BioGRID-ORCS; 36236; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Drip; fly.
DR GenomeRNAi; 36236; -.
DR PRO; PR:Q9V5Z7; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0015872; Expressed in seminal fluid secreting gland and 28 other tissues.
DR ExpressionAtlas; Q9V5Z7; baseline and differential.
DR Genevisible; Q9V5Z7; DM.
DR GO; GO:0016324; C:apical plasma membrane; IDA:FlyBase.
DR GO; GO:0016323; C:basolateral plasma membrane; IDA:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; IDA:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015250; F:water channel activity; IDA:FlyBase.
DR GO; GO:0046662; P:regulation of oviposition; IMP:FlyBase.
DR GO; GO:0003014; P:renal system process; IEP:FlyBase.
DR GO; GO:0055085; P:transmembrane transport; IDA:FlyBase.
DR GO; GO:0030104; P:water homeostasis; IEP:FlyBase.
DR GO; GO:0006833; P:water transport; IDA:FlyBase.
DR CDD; cd00333; MIP; 1.
DR Gene3D; 1.20.1080.10; -; 1.
DR InterPro; IPR023271; Aquaporin-like.
DR InterPro; IPR034294; Aquaporin_transptr.
DR InterPro; IPR000425; MIP.
DR InterPro; IPR022357; MIP_CS.
DR PANTHER; PTHR19139; PTHR19139; 1.
DR Pfam; PF00230; MIP; 1.
DR PRINTS; PR00783; MINTRINSICP.
DR SUPFAM; SSF81338; SSF81338; 1.
DR TIGRFAMs; TIGR00861; MIP; 1.
DR PROSITE; PS00221; MIP; 1.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Repeat; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..245
FT /note="Aquaporin"
FT /id="PRO_0000063976"
FT TOPO_DOM 1..26
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 27..46
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 47..51
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 52..72
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 73..94
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 116..132
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 154..163
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..205
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 206..226
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 227..245
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOTIF 76..78
FT /note="NPA 1"
FT MOTIF 192..194
FT /note="NPA 2"
FT CONFLICT 122..129
FT /note="GDLGVSSF -> ATWSILL (in Ref. 4; AAA81324)"
FT /evidence="ECO:0000305"
FT CONFLICT 191
FT /note="M -> T (in Ref. 4; AAA81324)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 245 AA; 25575 MW; 611AA17CFE3B807C CRC64;
MVEKTEMSKF VGVADITENK KIWRMLLGEL VGTFFLIFVG VGSTTSGSVP QIAFTFGLTV
ATIAQGLGHL SGCHINPAVT LGFLIVGEIS ILKAAFYIIV QCVGAIAGAA VIKVALDGVA
GGDLGVSSFD PSLNCAQAVL IEALITFILV FVVKAVSDPG RQDIKGSAPL AVGLAIAAGH
LCAIKLSGAS MNPARSFGPA VVQGVWTYHW VYWVGPIAGG LLAGIIYRLI FKVRKGDDET
DSYDF
