ID   AQP_NOSCE               Reviewed;         249 AA.
AC   C4VBN2;
DT   19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=Aquaporin;
GN   Name=AQP; ORFNames=NCER_102220;
OS   Nosema ceranae (strain BRL01) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Nosematidae; Nosema.
OX   NCBI_TaxID=578460;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BRL01;
RX   PubMed=19503607; DOI=10.1371/journal.ppat.1000466;
RA   Cornman R.S., Chen Y.P., Schatz M.C., Street C., Zhao Y., Desany B.,
RA   Egholm M., Hutchison S., Pettis J.S., Lipkin W.I., Evans J.D.;
RT   "Genomic analyses of the microsporidian Nosema ceranae, an emergent
RT   pathogen of honey bees.";
RL   PLoS Pathog. 5:E1000466-E1000466(2009).
CC   -!- FUNCTION: Water channel required to facilitate the transport of water
CC       across membranes. Involved in osmotolerance (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC       membrane-spanning domains and a pore-forming loop with the signature
CC       motif Asn-Pro-Ala (NPA). In microsporidia, the second signature motif
CC       differs slightly and is Asn-Pro-Gly (NPG).
CC   -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; ACOL01000674; EEQ81370.1; -; Genomic_DNA.
DR   RefSeq; XP_002995041.1; XM_002994995.1.
DR   AlphaFoldDB; C4VBN2; -.
DR   SMR; C4VBN2; -.
DR   STRING; 578460.C4VBN2; -.
DR   EnsemblFungi; EEQ81370; EEQ81370; NCER_102220.
DR   KEGG; nce:NCER_102220; -.
DR   VEuPathDB; MicrosporidiaDB:NCER_102220; -.
DR   HOGENOM; CLU_020019_3_4_1; -.
DR   InParanoid; C4VBN2; -.
DR   OMA; MSSRKLM; -.
DR   Proteomes; UP000009082; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015267; F:channel activity; IEA:InterPro.
DR   Gene3D; 1.20.1080.10; -; 1.
DR   InterPro; IPR023271; Aquaporin-like.
DR   InterPro; IPR034294; Aquaporin_transptr.
DR   InterPro; IPR000425; MIP.
DR   InterPro; IPR022357; MIP_CS.
DR   PANTHER; PTHR45665; PTHR45665; 1.
DR   Pfam; PF00230; MIP; 1.
DR   PRINTS; PR00783; MINTRINSICP.
DR   SUPFAM; SSF81338; SSF81338; 1.
DR   PROSITE; PS00221; MIP; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Reference proteome; Repeat; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..249
FT                   /note="Aquaporin"
FT                   /id="PRO_0000390774"
FT   TOPO_DOM        1..11
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        33..39
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        40..60
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        61..83
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        84..104
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        105..133
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        134..154
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        155..179
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        180..200
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        201..223
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        224..246
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        247..249
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   MOTIF           69..71
FT                   /note="NPA"
FT   MOTIF           206..208
FT                   /note="NPG"
SQ   SEQUENCE   249 AA;  26936 MW;  BEF40E5651D17F22 CRC64;
     MTRKWIKKLQ SYIGEFFASF IFGFAVYTSI IGSAQTGQSA GPIIVALTIA LSGVAIIYSF
     CDITVAHFNP AITFSAMCFR RLPFFGGIFI IIFQVAGFII AGLASVAVLP GKYKNKLEIA
     RPKRVADNVS RGRIFGTEFF LTAILVYVAF AVGVNPYTPP KDEHGDQLDP DEGLTEGRKI
     TAPLAIGFTL GFCALLGIAS SGGAFNPGIV LSPMILTGTW DFWWVYLLGQ FSGGLLGGGL
     QRFLLYKIF