ID   KDPE_STAA8              Reviewed;         231 AA.
AC   Q2FWH6;
DT   11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Transcriptional regulatory protein KdpE {ECO:0000303|PubMed:20498265};
GN   Name=kdpE {ECO:0000303|PubMed:20498265}; OrderedLocusNames=SAOUHSC_02315;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
RN   [2]
RP   FUNCTION, PHOSPHORYLATION, AND DISRUPTION PHENOTYPE.
RX   PubMed=20498265; DOI=10.1128/iai.00131-10;
RA   Zhao L., Xue T., Shang F., Sun H., Sun B.;
RT   "Staphylococcus aureus AI-2 quorum sensing associates with the KdpDE two-
RT   component system to regulate capsular polysaccharide synthesis and
RT   virulence.";
RL   Infect. Immun. 78:3506-3515(2010).
RN   [3]
RP   FUNCTION, AND INDUCTION BY AGR.
RX   PubMed=21422185; DOI=10.1128/iai.01180-10;
RA   Xue T., You Y., Hong D., Sun H., Sun B.;
RT   "The Staphylococcus aureus KdpDE two-component system couples extracellular
RT   K+ sensing and Agr signaling to infection programming.";
RL   Infect. Immun. 79:2154-2167(2011).
RN   [4]
RP   FUNCTION.
RX   PubMed=26195599; DOI=10.1128/jb.00480-15;
RA   Moscoso J.A., Schramke H., Zhang Y., Tosi T., Dehbi A., Jung K.,
RA   Gruendling A.;
RT   "Binding of Cyclic Di-AMP to the Staphylococcus aureus Sensor Kinase KdpD
RT   Occurs via the Universal Stress Protein Domain and Downregulates the
RT   Expression of the Kdp Potassium Transporter.";
RL   J. Bacteriol. 198:98-110(2016).
CC   -!- FUNCTION: Member of the two-component regulatory system KdpD/KdpE that
CC       regulates the transcription of a series of virulence factors through
CC       sensing external K(+) concentrations. Regulates also capsular
CC       polysaccharide synthesis (PubMed:20498265). Upon phosphorylation by
CC       KpdD, functions as a transcriptional regulator by direct binding to
CC       promoter regions of target genes including spa, hla, aur and geh
CC       (PubMed:21422185). Represses the transcription of kdpFABC operon
CC       (PubMed:26195599). {ECO:0000269|PubMed:20498265,
CC       ECO:0000269|PubMed:21422185, ECO:0000269|PubMed:26195599}.
CC   -!- INDUCTION: By the Agr/RNAIII system. {ECO:0000269|PubMed:21422185}.
CC   -!- PTM: Phosphorylated by KdpD. Phosphorylation is required for
CC       transcriptional activity. {ECO:0000269|PubMed:20498265}.
CC   -!- DISRUPTION PHENOTYPE: Inactivation of kdpE results in decreased
CC       transcript level of cap. {ECO:0000269|PubMed:20498265}.
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DR   EMBL; CP000253; ABD31349.1; -; Genomic_DNA.
DR   RefSeq; WP_001190841.1; NZ_LS483365.1.
DR   RefSeq; YP_500794.1; NC_007795.1.
DR   AlphaFoldDB; Q2FWH6; -.
DR   SMR; Q2FWH6; -.
DR   STRING; 1280.SAXN108_2324; -.
DR   EnsemblBacteria; ABD31349; ABD31349; SAOUHSC_02315.
DR   GeneID; 3920940; -.
DR   KEGG; sao:SAOUHSC_02315; -.
DR   PATRIC; fig|93061.5.peg.2098; -.
DR   eggNOG; COG0745; Bacteria.
DR   HOGENOM; CLU_000445_30_8_9; -.
DR   OMA; YIQTHIG; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR   GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Phosphoprotein; Reference proteome; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..231
FT                   /note="Transcriptional regulatory protein KdpE"
FT                   /id="PRO_0000448707"
FT   DOMAIN          4..117
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        127..227
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         53
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   231 AA;  26512 MW;  AA97CD9D7F3A5666 CRC64;
     MQSKILIIED DHAITHLLDV ALTLDYYNVT TADNATQAHF KIQIDKPDVI LLDLGLPDKD
     GLCLISEIRQ HTDIPIIVIS ARQEEQTIIQ ALDNGANDYM TKPFNVDELR ARIRVIERIA
     KSHQETNIVF TNGLLSIDFG SKSVVINNQE VHLTPNEFSL LELLSNHKGK VLTYEMILKR
     IYGYVNKTEM PSLRVHMTSL RQKLSQCHED AKDIIKTHPR IGYQMLQWKE K