ID   KDSA1_PSEPK             Reviewed;         281 AA.
AC   Q88MG0;
DT   20-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   20-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=2-dehydro-3-deoxyphosphooctonate aldolase 1;
DE            EC=2.5.1.55;
DE   AltName: Full=3-deoxy-D-manno-octulosonic acid 8-phosphate synthase 1;
DE   AltName: Full=KDO-8-phosphate synthase 1;
DE            Short=KDO 8-P synthase 1;
DE            Short=KDOPS 1;
DE   AltName: Full=Phospho-2-dehydro-3-deoxyoctonate aldolase 1;
GN   Name=kdsA1; Synonyms=kdsA-1; OrderedLocusNames=PP_1611;
OS   Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS   / KT2440).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA   Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA   Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA   Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA   Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA   Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the metabolically
RT   versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-arabinose 5-phosphate + H2O + phosphoenolpyruvate = 3-deoxy-
CC         alpha-D-manno-2-octulosonate-8-phosphate + phosphate;
CC         Xref=Rhea:RHEA:14053, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57693, ChEBI:CHEBI:58702, ChEBI:CHEBI:85985; EC=2.5.1.55;
CC   -!- PATHWAY: Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate
CC       biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate:
CC       step 2/3.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the KdsA family. {ECO:0000305}.
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DR   EMBL; AE015451; AAN67232.1; -; Genomic_DNA.
DR   RefSeq; NP_743768.1; NC_002947.4.
DR   RefSeq; WP_010952685.1; NC_002947.4.
DR   AlphaFoldDB; Q88MG0; -.
DR   SMR; Q88MG0; -.
DR   STRING; 160488.PP_1611; -.
DR   EnsemblBacteria; AAN67232; AAN67232; PP_1611.
DR   KEGG; ppu:PP_1611; -.
DR   PATRIC; fig|160488.4.peg.1702; -.
DR   eggNOG; COG2877; Bacteria.
DR   HOGENOM; CLU_036666_0_0_6; -.
DR   OMA; FRGIPTM; -.
DR   PhylomeDB; Q88MG0; -.
DR   BioCyc; PPUT160488:G1G01-1708-MON; -.
DR   UniPathway; UPA00030; -.
DR   UniPathway; UPA00357; UER00474.
DR   Proteomes; UP000000556; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008676; F:3-deoxy-8-phosphooctulonate synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019294; P:keto-3-deoxy-D-manno-octulosonic acid biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.20.20.70; -; 1.
DR   HAMAP; MF_00056; KDO8P_synth; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR006218; DAHP1/KDSA.
DR   InterPro; IPR006269; KDO8P_synthase.
DR   PANTHER; PTHR21057; PTHR21057; 1.
DR   Pfam; PF00793; DAHP_synth_1; 1.
DR   TIGRFAMs; TIGR01362; KDO8P_synth; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Lipopolysaccharide biosynthesis; Reference proteome;
KW   Transferase.
FT   CHAIN           1..281
FT                   /note="2-dehydro-3-deoxyphosphooctonate aldolase 1"
FT                   /id="PRO_0000187151"
SQ   SEQUENCE   281 AA;  31034 MW;  21C189C63F0C63CB CRC64;
     MTQKIIRVGN IEIANDKPFV LFGGMNVLES RDLAMKVCEE YVRVTEKLGI PYVFKASFDK
     ANRSSVTSYR GPGMEEGLKI FEEIKRTFNV PVITDVHEPY QAEPVAKVCD IIQLPAFLSR
     QTDLVVAMAK TGVVINIKKA QFLAPQEMKH ILAKCEEAGN DQLILCERGS SFGYNNLVVD
     MLGFGIMKQF EYPVFFDVTH ALQMPGGRAD SAGGRRAQVT DLAKAGMSQG LAGLFLEAHP
     DPDNAKCDGP CALRLDKLEP FLVQLKQLDD LVKSFPTVET A