KDSA2_ARATH
ID KDSA2_ARATH Reviewed; 291 AA.
AC Q6NQL4; Q9SA33;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=2-dehydro-3-deoxyphosphooctonate aldolase 2;
DE EC=2.5.1.55 {ECO:0000250|UniProtKB:Q9AV97};
DE AltName: Full=3-deoxy-D-manno-octulosonic acid 8-phosphate synthase;
DE AltName: Full=KDO-8-phosphate synthase 2;
DE Short=AtkdsA2;
DE Short=KDO 8-P synthase;
DE Short=KDOPS;
DE AltName: Full=Phospho-2-dehydro-3-deoxyoctonate aldolase;
GN Name=KDSA2; Synonyms=kdsA; OrderedLocusNames=At1g16340; ORFNames=F3O9.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=18503041; DOI=10.1093/jxb/ern118;
RA Delmas F., Seveno M., Northey J.G., Hernould M., Lerouge P., McCourt P.,
RA Chevalier C.;
RT "The synthesis of the rhamnogalacturonan II component 3-deoxy-D-manno-2-
RT octulosonic acid (Kdo) is required for pollen tube growth and elongation.";
RL J. Exp. Bot. 59:2639-2647(2008).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: Catalyzes the stereospecific condensation of D-arabinose 5-
CC phosphate and phosphoenolpyruvate to form 3-deoxy-D-manno-octulosonate
CC 8-phosphate (KDO-8-phosphate) and inorganic phosphate. Involved in the
CC biosynthesis of 3-deoxy-D-manno-octulosonate (KDO) which is an
CC indispensable component of rhamnogalacturonan II (RG-II), a
CC structurally complex pectic polysaccharide of the primary cell wall.
CC RG-II is essential for the cell wall integrity of rapidly growing
CC tissues and pollen tube growth and elongation.
CC {ECO:0000269|PubMed:18503041}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-arabinose 5-phosphate + H2O + phosphoenolpyruvate = 3-deoxy-
CC alpha-D-manno-2-octulosonate-8-phosphate + phosphate;
CC Xref=Rhea:RHEA:14053, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57693, ChEBI:CHEBI:58702, ChEBI:CHEBI:85985; EC=2.5.1.55;
CC Evidence={ECO:0000250|UniProtKB:Q9AV97};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q6NQL4-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in roots, apical meristem, emerging
CC leaves, hydathodes of young leaves, styles of mature flowers and
CC funicules of mature siliques. {ECO:0000269|PubMed:18503041}.
CC -!- SIMILARITY: Belongs to the KdsA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD34685.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006341; AAD34685.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29437.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29438.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29439.1; -; Genomic_DNA.
DR EMBL; BT010438; AAQ62439.1; -; mRNA.
DR EMBL; AK175407; BAD43170.1; -; mRNA.
DR PIR; E86298; E86298.
DR RefSeq; NP_001031055.1; NM_001035978.2. [Q6NQL4-1]
DR RefSeq; NP_001077547.1; NM_001084078.1. [Q6NQL4-1]
DR RefSeq; NP_173084.1; NM_101500.4. [Q6NQL4-1]
DR AlphaFoldDB; Q6NQL4; -.
DR SMR; Q6NQL4; -.
DR BioGRID; 23443; 1.
DR STRING; 3702.AT1G16340.4; -.
DR iPTMnet; Q6NQL4; -.
DR MetOSite; Q6NQL4; -.
DR PaxDb; Q6NQL4; -.
DR PRIDE; Q6NQL4; -.
DR ProteomicsDB; 250757; -. [Q6NQL4-1]
DR EnsemblPlants; AT1G16340.1; AT1G16340.1; AT1G16340. [Q6NQL4-1]
DR EnsemblPlants; AT1G16340.2; AT1G16340.2; AT1G16340. [Q6NQL4-1]
DR EnsemblPlants; AT1G16340.3; AT1G16340.3; AT1G16340. [Q6NQL4-1]
DR GeneID; 838203; -.
DR Gramene; AT1G16340.1; AT1G16340.1; AT1G16340. [Q6NQL4-1]
DR Gramene; AT1G16340.2; AT1G16340.2; AT1G16340. [Q6NQL4-1]
DR Gramene; AT1G16340.3; AT1G16340.3; AT1G16340. [Q6NQL4-1]
DR KEGG; ath:AT1G16340; -.
DR Araport; AT1G16340; -.
DR eggNOG; ENOG502QQN7; Eukaryota.
DR InParanoid; Q6NQL4; -.
DR OMA; IKKPQFM; -.
DR PhylomeDB; Q6NQL4; -.
DR BioCyc; ARA:AT1G16340-MON; -.
DR BRENDA; 2.5.1.55; 399.
DR PRO; PR:Q6NQL4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q6NQL4; baseline and differential.
DR Genevisible; Q6NQL4; AT.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0008676; F:3-deoxy-8-phosphooctulonate synthase activity; IDA:UniProtKB.
DR GO; GO:0046364; P:monosaccharide biosynthetic process; IDA:UniProtKB.
DR GO; GO:0048868; P:pollen tube development; IMP:UniProtKB.
DR GO; GO:0009860; P:pollen tube growth; IMP:UniProtKB.
DR GO; GO:0010306; P:rhamnogalacturonan II biosynthetic process; TAS:UniProtKB.
DR Gene3D; 3.20.20.70; -; 1.
DR HAMAP; MF_00056; KDO8P_synth; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR006218; DAHP1/KDSA.
DR InterPro; IPR006269; KDO8P_synthase.
DR PANTHER; PTHR21057; PTHR21057; 1.
DR Pfam; PF00793; DAHP_synth_1; 1.
DR TIGRFAMs; TIGR01362; KDO8P_synth; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cell wall biogenesis/degradation;
KW Cytoplasm; Reference proteome; Transferase.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..291
FT /note="2-dehydro-3-deoxyphosphooctonate aldolase 2"
FT /id="PRO_0000421463"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22223895"
SQ SEQUENCE 291 AA; 31676 MW; E0EEC13A56AD9FAC CRC64;
MANSASLLYD QLKVAEPFFL LAGPNVIESE EHVLRMAKSI KDISTKLGLP LVFKSSFDKA
NRTSSKSFRG PGMAEGLKIL EKVKVAFDLP IVTDVHESSQ CEAVGKVADI IQIPAFLCRQ
TDLLVAAAQS GKIINIKKGQ FCGHSVMRNS AEKVRLAGNP NVMVCERGTM FGYNDLIVDP
RNLEWMREAD CPVVADITHS LQQPAGKKLD GGGVASGGLR ELIPCIARTA VAVGVDGIFM
EVHDDPLNAP VDGPTQWPLR HLEELLEELI AIASVTKGKQ QFQIDLTPYR D
