ID   KDSA2_PSEPK             Reviewed;         280 AA.
AC   Q88LX0;
DT   24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=2-dehydro-3-deoxyphosphooctonate aldolase 2;
DE            EC=2.5.1.55;
DE   AltName: Full=3-deoxy-D-manno-octulosonic acid 8-phosphate synthase 2;
DE   AltName: Full=KDO-8-phosphate synthase 2;
DE            Short=KDO 8-P synthase 2;
DE            Short=KDOPS 2;
DE   AltName: Full=Phospho-2-dehydro-3-deoxyoctonate aldolase 2;
GN   Name=kdsA2; Synonyms=kdsA-2; OrderedLocusNames=PP_1807;
OS   Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS   / KT2440).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA   Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA   Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA   Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA   Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA   Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the metabolically
RT   versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-arabinose 5-phosphate + H2O + phosphoenolpyruvate = 3-deoxy-
CC         alpha-D-manno-2-octulosonate-8-phosphate + phosphate;
CC         Xref=Rhea:RHEA:14053, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57693, ChEBI:CHEBI:58702, ChEBI:CHEBI:85985; EC=2.5.1.55;
CC   -!- PATHWAY: Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate
CC       biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate:
CC       step 2/3.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the KdsA family. {ECO:0000305}.
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DR   EMBL; AE015451; AAN67426.1; -; Genomic_DNA.
DR   RefSeq; NP_743962.1; NC_002947.4.
DR   RefSeq; WP_010952843.1; NC_002947.4.
DR   AlphaFoldDB; Q88LX0; -.
DR   SMR; Q88LX0; -.
DR   STRING; 160488.PP_1807; -.
DR   EnsemblBacteria; AAN67426; AAN67426; PP_1807.
DR   KEGG; ppu:PP_1807; -.
DR   PATRIC; fig|160488.4.peg.1906; -.
DR   eggNOG; COG2877; Bacteria.
DR   HOGENOM; CLU_036666_0_0_6; -.
DR   OMA; IKKPQFM; -.
DR   PhylomeDB; Q88LX0; -.
DR   BioCyc; PPUT160488:G1G01-1910-MON; -.
DR   UniPathway; UPA00030; -.
DR   UniPathway; UPA00357; UER00474.
DR   Proteomes; UP000000556; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008676; F:3-deoxy-8-phosphooctulonate synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019294; P:keto-3-deoxy-D-manno-octulosonic acid biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.20.20.70; -; 1.
DR   HAMAP; MF_00056; KDO8P_synth; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR006218; DAHP1/KDSA.
DR   InterPro; IPR006269; KDO8P_synthase.
DR   PANTHER; PTHR21057; PTHR21057; 1.
DR   Pfam; PF00793; DAHP_synth_1; 1.
DR   TIGRFAMs; TIGR01362; KDO8P_synth; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Lipopolysaccharide biosynthesis; Reference proteome;
KW   Transferase.
FT   CHAIN           1..280
FT                   /note="2-dehydro-3-deoxyphosphooctonate aldolase 2"
FT                   /id="PRO_0000187152"
SQ   SEQUENCE   280 AA;  30469 MW;  1300418DC9803F4E CRC64;
     MIKINSTIEC SNSAPFVLFG GINVLESEDL ALTACAEYVR VTQKLGIPYV FKASFDKANR
     SSIHSYRGPG MEEGLRIFEK VKAEFGVPII TDVHEIYQTA PVAEVVDVLQ LPAFLARQTD
     LVVALAKTGK PVNIKKPQFL SPSQMQNIVH KFKEAGNDQL ILCDRGTCMG YDNLIVDMLG
     FGVMKRTCQD LPIIFDVTHA LQNRDPSGAA SGGRREQVVE LARAGMGVGL AGLFLEAHPN
     PDQAKCDGPS ALPLDKLEPF LAQIKALDDL VKSFPQLTIA