ID   53DR_BPKV4              Reviewed;         170 AA.
AC   P59935;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Putative 5'(3')-deoxyribonucleotidase;
DE            EC=3.1.3.-;
GN   OrderedLocusNames=KVP40.0071;
OS   Vibrio phage KVP40 (Bacteriophage KVP40).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Schizotequatrovirus.
OX   NCBI_TaxID=75320;
OH   NCBI_TaxID=662; Vibrio.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12923095; DOI=10.1128/jb.185.17.5220-5233.2003;
RA   Miller E.S., Heidelberg J.F., Eisen J.A., Nelson W.C., Durkin A.S.,
RA   Ciecko A., Feldblyum T.V., White O., Paulsen I.T., Nierman W.C., Lee J.,
RA   Szczypinski B., Fraser C.M.;
RT   "Complete genome sequence of the broad-host-range vibriophage KVP40:
RT   comparative genomics of a T4-related bacteriophage.";
RL   J. Bacteriol. 185:5220-5233(2003).
CC   -!- FUNCTION: Dephosphorylates the 5' and 2'(3')-phosphates of
CC       deoxyribonucleotides. {ECO:0000305}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:Q97JQ5};
CC   -!- SIMILARITY: Belongs to the 5'(3')-deoxyribonucleotidase family.
CC       {ECO:0000305}.
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DR   EMBL; AY283928; AAQ64142.1; -; Genomic_DNA.
DR   RefSeq; NP_899319.1; NC_005083.2.
DR   SMR; P59935; -.
DR   GeneID; 2545825; -.
DR   KEGG; vg:2545825; -.
DR   GO; GO:0008253; F:5'-nucleotidase activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009264; P:deoxyribonucleotide catabolic process; IEA:InterPro.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR010708; 5'(3')-deoxyribonucleotidase.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR023214; HAD_sf.
DR   Pfam; PF06941; NT5C; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding.
FT   CHAIN           1..170
FT                   /note="Putative 5'(3')-deoxyribonucleotidase"
FT                   /id="PRO_0000164388"
FT   ACT_SITE        28
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000305"
FT   ACT_SITE        30
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000305"
FT   BINDING         28
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:Q8CTG7"
FT   BINDING         30
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:Q8CTG7"
FT   BINDING         134
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:Q8CTG7"
SQ   SEQUENCE   170 AA;  19766 MW;  AA26FABD620DF1E1 CRC64;
     MKLMNVVKLM IRAIARAKKT DKPIVYIDMD NVLVDFQSGI DSLSIWEKNQ YEGRYDECPG
     IFAHMKPMKG AIAAFEVLNK HFDCYILSTA PWDNPGSWQD KRLWVERYLG KGAYKRLILS
     HHKQLNFGHY LIDDRTKNGA GEFMGEHIHF GTDEFPDWFS VVTYLTSENK