AR13B_DANRE
ID AR13B_DANRE Reviewed; 407 AA.
AC Q8JHI3; Q561Z5;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=ADP-ribosylation factor-like protein 13B;
DE AltName: Full=ADP-ribosylation factor-like protein 2-like 1;
DE Short=ARL2-like protein 1;
DE AltName: Full=Protein scorpion;
GN Name=arl13b; Synonyms=arl2l1;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND FUNCTION.
RC TISSUE=Embryo;
RX PubMed=12006978; DOI=10.1038/ng896;
RA Golling G., Amsterdam A., Sun Z., Antonelli M., Maldonado E., Chen W.,
RA Burgess S., Haldi M., Artzt K., Farrington S., Lin S.-Y., Nissen R.M.,
RA Hopkins N.;
RT "Insertional mutagenesis in zebrafish rapidly identifies genes essential
RT for early vertebrate development.";
RL Nat. Genet. 31:135-140(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-275.
RC TISSUE=Olfactory epithelium;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15269167; DOI=10.1242/dev.01240;
RA Sun Z., Amsterdam A., Pazour G.J., Cole D.G., Miller M.S., Hopkins N.;
RT "A genetic screen in zebrafish identifies cilia genes as a principal cause
RT of cystic kidney.";
RL Development 131:4085-4093(2004).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND MUTAGENESIS OF GLY-28 AND THR-35.
RX PubMed=19906870; DOI=10.1242/dev.036350;
RA Duldulao N.A., Lee S., Sun Z.;
RT "Cilia localization is essential for in vivo functions of the Joubert
RT syndrome protein Arl13b/Scorpion.";
RL Development 136:4033-4042(2009).
CC -!- FUNCTION: Cilium-specific protein required to control the microtubule-
CC based, ciliary axoneme structure. May act by maintaining the
CC association between IFT subcomplexes A and B. Binds GTP but is not able
CC to hydrolyze it; the GTPase activity remains unclear. Required to
CC pattern the neural tube. Involved in cerebral cortex development:
CC required for the initial formation of a polarized radial glial
CC scaffold, the first step in the construction of the cerebral cortex, by
CC regulating ciliary signaling. Regulates the migration and placement of
CC postmitotic interneurons in the developing cerebral cortex Required
CC during embryogenesis for cilia formation in multiple organs such as the
CC pronephric duct. {ECO:0000269|PubMed:12006978,
CC ECO:0000269|PubMed:15269167, ECO:0000269|PubMed:19906870}.
CC -!- SUBUNIT: Interacts with EXOC2; regulates ARL13B localization to the
CC cilium membrane. {ECO:0000250|UniProtKB:Q640N2}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium membrane
CC {ECO:0000269|PubMed:19906870}; Lipid-anchor
CC {ECO:0000269|PubMed:19906870}. Note=Associates to the cilium membrane
CC via palmitoylation. Localizes to proximal ciliary membranes, to an
CC inversin-like subciliary membrane compartment, excluding the transition
CC zone.
CC -!- TISSUE SPECIFICITY: Cilium-specific. {ECO:0000269|PubMed:19906870}.
CC -!- DEVELOPMENTAL STAGE: Detected at the 4- to 32-cell stage, suggesting it
CC is expressed maternally. {ECO:0000269|PubMed:19906870}.
CC -!- DISRUPTION PHENOTYPE: Embryos have a ventrally curved tail, ciliary
CC defects and cystic kidneys. {ECO:0000269|PubMed:15269167}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Arf family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH92689.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};
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DR EMBL; AF506213; AAM34657.1; -; mRNA.
DR EMBL; BC092689; AAH92689.1; ALT_SEQ; mRNA.
DR RefSeq; NP_775379.1; NM_173272.1.
DR AlphaFoldDB; Q8JHI3; -.
DR SMR; Q8JHI3; -.
DR STRING; 7955.ENSDARP00000092960; -.
DR PaxDb; Q8JHI3; -.
DR Ensembl; ENSDART00000102184; ENSDARP00000092960; ENSDARG00000012763.
DR GeneID; 286784; -.
DR KEGG; dre:286784; -.
DR CTD; 200894; -.
DR ZFIN; ZDB-GENE-021217-3; arl13b.
DR eggNOG; KOG0074; Eukaryota.
DR eggNOG; KOG0076; Eukaryota.
DR GeneTree; ENSGT00940000156365; -.
DR HOGENOM; CLU_040729_3_0_1; -.
DR InParanoid; Q8JHI3; -.
DR OrthoDB; 732329at2759; -.
DR PhylomeDB; Q8JHI3; -.
DR TreeFam; TF105476; -.
DR Reactome; R-DRE-5624958; ARL13B-mediated ciliary trafficking of INPP5E.
DR Reactome; R-DRE-9013406; RHOQ GTPase cycle.
DR Reactome; R-DRE-9013409; RHOJ GTPase cycle.
DR Reactome; R-DRE-9646399; Aggrephagy.
DR PRO; PR:Q8JHI3; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 1.
DR Bgee; ENSDARG00000012763; Expressed in testis and 28 other tissues.
DR ExpressionAtlas; Q8JHI3; baseline.
DR GO; GO:0060170; C:ciliary membrane; ISS:UniProtKB.
DR GO; GO:0005929; C:cilium; IDA:ZFIN.
DR GO; GO:0031514; C:motile cilium; IBA:GO_Central.
DR GO; GO:0097730; C:non-motile cilium; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0060271; P:cilium assembly; IMP:ZFIN.
DR GO; GO:0021943; P:formation of radial glial scaffolds; ISS:UniProtKB.
DR GO; GO:0021830; P:interneuron migration from the subpallium to the cortex; ISS:UniProtKB.
DR GO; GO:0021532; P:neural tube patterning; ISS:UniProtKB.
DR GO; GO:1905515; P:non-motile cilium assembly; ISS:UniProtKB.
DR GO; GO:0035845; P:photoreceptor cell outer segment organization; IMP:ZFIN.
DR GO; GO:0097500; P:receptor localization to non-motile cilium; IBA:GO_Central.
DR GO; GO:0007224; P:smoothened signaling pathway; ISS:UniProtKB.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR006689; Small_GTPase_ARF/SAR.
DR Pfam; PF00025; Arf; 1.
DR PRINTS; PR00328; SAR1GTPBP.
DR SMART; SM00178; SAR; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51417; ARF; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cell projection; Cilium; Coiled coil; Developmental protein;
KW GTP-binding; Lipoprotein; Membrane; Nucleotide-binding; Palmitate;
KW Reference proteome.
FT CHAIN 1..407
FT /note="ADP-ribosylation factor-like protein 13B"
FT /id="PRO_0000251139"
FT REGION 202..407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 195..313
FT /evidence="ECO:0000255"
FT COMPBIAS 202..253
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 296..312
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 28..35
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 71..75
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 130..133
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT LIPID 8
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250"
FT LIPID 9
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250"
FT MUTAGEN 28
FT /note="G->V: Abolishes ability to rescue a arl13b mutant
FT when transfected, without affecting localization to
FT cilium."
FT /evidence="ECO:0000269|PubMed:19906870"
FT MUTAGEN 35
FT /note="T->N: Abolishes ability to rescue a arl13b mutant
FT when transfected, without affecting localization to
FT cilium."
FT /evidence="ECO:0000269|PubMed:19906870"
FT CONFLICT 43
FT /note="G -> E (in Ref. 2; AAH92689)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 407 AA; 46566 MW; DE491E88F57FAEC9 CRC64;
MFNLMANCCN WLKRWREPAR KVTLVMVGLD NAGKTATVRG IQGESPLDVA PTVGFSKVDL
KQGKFEVTIF DLGGGKRIRG IWKNYYSESY GVVFVVDSSD VQRIQETRDT MAEVLRHPRI
AGKPVLVLAN KQDQDGAMAE ADIIETLSLE KLVNENKCLC QIEPCSAVLG YGKKVDKSIK
NGLNWLLNNI AKDYEAISER VQKDTAEQKA QEEQDKKERA ERVRRIREER DRQEREEAER
EGRTLKEEEL DDVNMFNPFQ PINNVLTENQ DRLNREKEMQ RQRENGQQGS VQEQIALQDE
EEEEEDEESE RQTPESTESG AVDQTKKKTR KLRLKRKHRV DPLRMEEAAP KSPTPPPLPV
GWATPKVSRL PKLEPLGDTR HSDFYGKPLP PVAIRQRPNS DTHDVIS
