ID   KDSB2_BURCM             Reviewed;         267 AA.
AC   Q0B295;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=3-deoxy-manno-octulosonate cytidylyltransferase 2 {ECO:0000255|HAMAP-Rule:MF_00057};
DE            EC=2.7.7.38 {ECO:0000255|HAMAP-Rule:MF_00057};
DE   AltName: Full=CMP-2-keto-3-deoxyoctulosonic acid synthase 2 {ECO:0000255|HAMAP-Rule:MF_00057};
DE            Short=CKS 2 {ECO:0000255|HAMAP-Rule:MF_00057};
DE            Short=CMP-KDO synthase 2 {ECO:0000255|HAMAP-Rule:MF_00057};
GN   Name=kdsB2 {ECO:0000255|HAMAP-Rule:MF_00057}; OrderedLocusNames=Bamb_6184;
OS   Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia
OS   (strain AMMD)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=339670;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-244 / AMMD;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P.,
RA   Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K.,
RA   Ramette A., Tiedje J., Richardson P.;
RT   "Complete sequence of chromosome 3 of Burkholderia cepacia AMMD.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Activates KDO (a required 8-carbon sugar) for incorporation
CC       into bacterial lipopolysaccharide in Gram-negative bacteria.
CC       {ECO:0000255|HAMAP-Rule:MF_00057}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-deoxy-alpha-D-manno-oct-2-ulosonate + CTP = CMP-3-deoxy-
CC         beta-D-manno-octulosonate + diphosphate; Xref=Rhea:RHEA:23448,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:37563, ChEBI:CHEBI:85986,
CC         ChEBI:CHEBI:85987; EC=2.7.7.38; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00057};
CC   -!- PATHWAY: Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-
CC       octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-
CC       deoxy-D-manno-octulosonate and CTP: step 1/1. {ECO:0000255|HAMAP-
CC       Rule:MF_00057}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00057}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00057}.
CC   -!- SIMILARITY: Belongs to the KdsB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00057}.
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DR   EMBL; CP000442; ABI91728.1; -; Genomic_DNA.
DR   RefSeq; WP_011661059.1; NZ_CP009800.1.
DR   AlphaFoldDB; Q0B295; -.
DR   SMR; Q0B295; -.
DR   STRING; 339670.Bamb_6184; -.
DR   EnsemblBacteria; ABI91728; ABI91728; Bamb_6184.
DR   GeneID; 44696720; -.
DR   KEGG; bam:Bamb_6184; -.
DR   PATRIC; fig|339670.21.peg.6045; -.
DR   eggNOG; COG1212; Bacteria.
DR   OMA; MSIKEHE; -.
DR   UniPathway; UPA00030; -.
DR   UniPathway; UPA00358; UER00476.
DR   Proteomes; UP000000662; Chromosome 3.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008690; F:3-deoxy-manno-octulosonate cytidylyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0033468; P:CMP-keto-3-deoxy-D-manno-octulosonic acid biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd02517; CMP-KDO-Synthetase; 1.
DR   Gene3D; 3.90.550.10; -; 1.
DR   HAMAP; MF_00057; KdsB; 1.
DR   InterPro; IPR003329; Cytidylyl_trans.
DR   InterPro; IPR004528; KdsB.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF02348; CTP_transf_3; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
DR   TIGRFAMs; TIGR00466; kdsB; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Lipopolysaccharide biosynthesis; Nucleotidyltransferase;
KW   Transferase.
FT   CHAIN           1..267
FT                   /note="3-deoxy-manno-octulosonate cytidylyltransferase 2"
FT                   /id="PRO_0000370020"
SQ   SEQUENCE   267 AA;  28659 MW;  52E71EA073465910 CRC64;
     MTSFNDGRTV RVVIPARYGS TRLPGKPLVD LDGEPMIVRV HARVRRALPD ADIVVAIDDA
     RIAEVLDARG IRFAMTDTGH ASGTDRAAEV ARVSGWPDTD AVLNVQGDEP LVPTALLQAF
     AGFCGAAPDL GVATVACPID EVALLDEPAI VKVVVDHRGR ALYFSRAAIP FCRDGRPADS
     AGSHGLFLRH IGLYGYANAT LQALASAAPC ELEQLEKLEQ LRALWLGMPI DVMRWPDAPP
     AGIDTPDDVA RVVSLLKRQT HDATEPY