AR6P4_MOUSE
ID AR6P4_MOUSE Reviewed; 229 AA.
AC Q9JM93; Q9WUG8;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=ADP-ribosylation factor-like protein 6-interacting protein 4;
DE Short=ARL-6-interacting protein 4;
DE Short=Aip-4;
DE AltName: Full=Splicing factor SRrp37;
GN Name=Arl6ip4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=10708573; DOI=10.1006/bbrc.2000.2301;
RA Sasahara K., Yamaoka T., Moritani M., Tanaka M., Iwahana H., Yoshimoto K.,
RA Miyagawa J., Kuroda Y., Itakura M.;
RT "Molecular cloning and expression analysis of a putative nuclear protein,
RT SR-25.";
RL Biochem. Biophys. Res. Commun. 269:444-450(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 114-177, AND INTERACTION WITH ARL6.
RX PubMed=10508919; DOI=10.1016/s0014-5793(99)01188-6;
RA Ingley E., Williams J.H., Walker C.E., Tsai S., Colley S., Sayer M.S.,
RA Tilbrook P.A., Sarna M., Beaumont J.G., Klinken S.P.;
RT "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-
RT conducting channel SEC61beta subunit.";
RL FEBS Lett. 459:69-74(1999).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Liver, Lung, Pancreas, Spleen,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Involved in modulating alternative pre-mRNA splicing with
CC either 5' distal site activation or preferential use of 3' proximal
CC site. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with ZCCHC17 (By similarity). Interacts with SRSF2
CC (By similarity). Interacts with ARL6. {ECO:0000250,
CC ECO:0000269|PubMed:10508919}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}. Nucleus speckle
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Widely expressed. Expressed at high level in testis
CC and thymus. {ECO:0000269|PubMed:10708573}.
CC -!- SIMILARITY: Belongs to the ARL6IP4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD33049.1; Type=Miscellaneous discrepancy; Note=Low quality sequence that contains many discrepancies.; Evidence={ECO:0000305};
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DR EMBL; AB035383; BAA94743.1; -; mRNA.
DR EMBL; AK133934; BAE21935.1; -; mRNA.
DR EMBL; BC063748; AAH63748.1; -; mRNA.
DR EMBL; AF133911; AAD33049.1; ALT_SEQ; mRNA.
DR CCDS; CCDS19673.1; -.
DR PIR; JC7219; JC7219.
DR RefSeq; NP_653092.1; NM_144509.2.
DR AlphaFoldDB; Q9JM93; -.
DR BioGRID; 211131; 3.
DR IntAct; Q9JM93; 2.
DR MINT; Q9JM93; -.
DR STRING; 10090.ENSMUSP00000031351; -.
DR iPTMnet; Q9JM93; -.
DR PhosphoSitePlus; Q9JM93; -.
DR EPD; Q9JM93; -.
DR jPOST; Q9JM93; -.
DR MaxQB; Q9JM93; -.
DR PaxDb; Q9JM93; -.
DR PRIDE; Q9JM93; -.
DR ProteomicsDB; 296276; -.
DR Antibodypedia; 31741; 93 antibodies from 23 providers.
DR DNASU; 65105; -.
DR Ensembl; ENSMUST00000031351; ENSMUSP00000031351; ENSMUSG00000029404.
DR GeneID; 65105; -.
DR KEGG; mmu:65105; -.
DR UCSC; uc008zoz.1; mouse.
DR CTD; 51329; -.
DR MGI; MGI:1929500; Arl6ip4.
DR VEuPathDB; HostDB:ENSMUSG00000029404; -.
DR eggNOG; ENOG502RYBZ; Eukaryota.
DR GeneTree; ENSGT00390000009670; -.
DR InParanoid; Q9JM93; -.
DR OMA; FQMRTGM; -.
DR OrthoDB; 1479900at2759; -.
DR PhylomeDB; Q9JM93; -.
DR TreeFam; TF350468; -.
DR BioGRID-ORCS; 65105; 5 hits in 72 CRISPR screens.
DR ChiTaRS; Arl6ip4; mouse.
DR PRO; PR:Q9JM93; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q9JM93; protein.
DR Bgee; ENSMUSG00000029404; Expressed in floor plate of midbrain and 267 other tissues.
DR ExpressionAtlas; Q9JM93; baseline and differential.
DR Genevisible; Q9JM93; MM.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR InterPro; IPR019532; Nucl_RNA-splicing_assoc_SR-25.
DR Pfam; PF10500; SR-25; 1.
PE 1: Evidence at protein level;
KW Isopeptide bond; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Ubl conjugation.
FT CHAIN 1..229
FT /note="ADP-ribosylation factor-like protein 6-interacting
FT protein 4"
FT /id="PRO_0000312271"
FT REGION 1..152
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..25
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 65..87
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..118
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 140
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 174
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q66PJ3"
FT CROSSLNK 191
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q66PJ3"
SQ SEQUENCE 229 AA; 25525 MW; 1F3D553DAD5E84F9 CRC64;
MAHVGSRKRS RSRSRSRSGR RGSEKRSKRS SKDASRNCSA SRSQGHKAGS ASGVEERSKH
KAQRTSRSSS TSSSSSSSSS ASSSSSSDGR KKRAKHKEKK RKKKKKKRKK KLKKRVKEKA
VAVHQAEALP GPSLDQWHRS AGEDNDGPVL TDEQKSRIQA MKPMTKEEWD ARQSVIRKVV
DPETGRTRLI KGDGEVLEEI VTKERHREIN KQATRGDGLA FQMRTGLLP
