KDSB_YERPE
ID KDSB_YERPE Reviewed; 250 AA.
AC Q8ZGA4; Q0WH15;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 129.
DE RecName: Full=3-deoxy-manno-octulosonate cytidylyltransferase {ECO:0000255|HAMAP-Rule:MF_00057};
DE EC=2.7.7.38 {ECO:0000255|HAMAP-Rule:MF_00057};
DE AltName: Full=CMP-2-keto-3-deoxyoctulosonic acid synthase {ECO:0000255|HAMAP-Rule:MF_00057};
DE Short=CKS {ECO:0000255|HAMAP-Rule:MF_00057};
DE Short=CMP-KDO synthase {ECO:0000255|HAMAP-Rule:MF_00057};
GN Name=kdsB {ECO:0000255|HAMAP-Rule:MF_00057};
GN OrderedLocusNames=YPO1400, y2772, YP_1193;
OS Yersinia pestis.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=632;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CO-92 / Biovar Orientalis;
RX PubMed=11586360; DOI=10.1038/35097083;
RA Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T.,
RA Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S.,
RA Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA Stevens K., Whitehead S., Barrell B.G.;
RT "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL Nature 413:523-527(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KIM10+ / Biovar Mediaevalis;
RX PubMed=12142430; DOI=10.1128/jb.184.16.4601-4611.2002;
RA Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P.,
RA Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D.,
RA Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A.,
RA Nilles M.L., Matson J.S., Blattner F.R., Perry R.D.;
RT "Genome sequence of Yersinia pestis KIM.";
RL J. Bacteriol. 184:4601-4611(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=91001 / Biovar Mediaevalis;
RX PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L.,
RA Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H.,
RA Wang J., Huang P., Yang R.;
RT "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT avirulent to humans.";
RL DNA Res. 11:179-197(2004).
CC -!- FUNCTION: Activates KDO (a required 8-carbon sugar) for incorporation
CC into bacterial lipopolysaccharide in Gram-negative bacteria.
CC {ECO:0000255|HAMAP-Rule:MF_00057}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-deoxy-alpha-D-manno-oct-2-ulosonate + CTP = CMP-3-deoxy-
CC beta-D-manno-octulosonate + diphosphate; Xref=Rhea:RHEA:23448,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:37563, ChEBI:CHEBI:85986,
CC ChEBI:CHEBI:85987; EC=2.7.7.38; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00057};
CC -!- PATHWAY: Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-
CC octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-
CC deoxy-D-manno-octulosonate and CTP: step 1/1. {ECO:0000255|HAMAP-
CC Rule:MF_00057}.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00057}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00057}.
CC -!- SIMILARITY: Belongs to the KdsB family. {ECO:0000255|HAMAP-
CC Rule:MF_00057}.
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DR EMBL; AL590842; CAL20052.1; -; Genomic_DNA.
DR EMBL; AE009952; AAM86324.1; -; Genomic_DNA.
DR EMBL; AE017042; AAS61436.1; -; Genomic_DNA.
DR PIR; AB0171; AB0171.
DR RefSeq; WP_002211314.1; NZ_WUCM01000045.1.
DR RefSeq; YP_002346423.1; NC_003143.1.
DR PDB; 3JTJ; X-ray; 2.18 A; A=1-250.
DR PDBsum; 3JTJ; -.
DR AlphaFoldDB; Q8ZGA4; -.
DR SMR; Q8ZGA4; -.
DR STRING; 214092.YPO1400; -.
DR PaxDb; Q8ZGA4; -.
DR DNASU; 1147719; -.
DR EnsemblBacteria; AAM86324; AAM86324; y2772.
DR EnsemblBacteria; AAS61436; AAS61436; YP_1193.
DR GeneID; 66842140; -.
DR KEGG; ype:YPO1400; -.
DR KEGG; ypk:y2772; -.
DR KEGG; ypm:YP_1193; -.
DR PATRIC; fig|214092.21.peg.1724; -.
DR eggNOG; COG1212; Bacteria.
DR HOGENOM; CLU_065038_1_0_6; -.
DR OMA; FMATCAK; -.
DR UniPathway; UPA00030; -.
DR UniPathway; UPA00358; UER00476.
DR EvolutionaryTrace; Q8ZGA4; -.
DR Proteomes; UP000000815; Chromosome.
DR Proteomes; UP000001019; Chromosome.
DR Proteomes; UP000002490; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0008690; F:3-deoxy-manno-octulosonate cytidylyltransferase activity; IBA:GO_Central.
DR GO; GO:0033468; P:CMP-keto-3-deoxy-D-manno-octulosonic acid biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd02517; CMP-KDO-Synthetase; 1.
DR Gene3D; 3.90.550.10; -; 1.
DR HAMAP; MF_00057; KdsB; 1.
DR InterPro; IPR003329; Cytidylyl_trans.
DR InterPro; IPR004528; KdsB.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF02348; CTP_transf_3; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
DR TIGRFAMs; TIGR00466; kdsB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Lipopolysaccharide biosynthesis;
KW Nucleotidyltransferase; Reference proteome; Transferase.
FT CHAIN 1..250
FT /note="3-deoxy-manno-octulosonate cytidylyltransferase"
FT /id="PRO_0000188521"
FT STRAND 4..9
FT /evidence="ECO:0007829|PDB:3JTJ"
FT STRAND 14..16
FT /evidence="ECO:0007829|PDB:3JTJ"
FT HELIX 19..21
FT /evidence="ECO:0007829|PDB:3JTJ"
FT HELIX 29..39
FT /evidence="ECO:0007829|PDB:3JTJ"
FT STRAND 43..50
FT /evidence="ECO:0007829|PDB:3JTJ"
FT HELIX 52..60
FT /evidence="ECO:0007829|PDB:3JTJ"
FT STRAND 64..67
FT /evidence="ECO:0007829|PDB:3JTJ"
FT HELIX 75..86
FT /evidence="ECO:0007829|PDB:3JTJ"
FT STRAND 93..96
FT /evidence="ECO:0007829|PDB:3JTJ"
FT HELIX 106..118
FT /evidence="ECO:0007829|PDB:3JTJ"
FT STRAND 119..129
FT /evidence="ECO:0007829|PDB:3JTJ"
FT HELIX 133..136
FT /evidence="ECO:0007829|PDB:3JTJ"
FT STRAND 143..146
FT /evidence="ECO:0007829|PDB:3JTJ"
FT STRAND 150..158
FT /evidence="ECO:0007829|PDB:3JTJ"
FT HELIX 164..169
FT /evidence="ECO:0007829|PDB:3JTJ"
FT STRAND 179..188
FT /evidence="ECO:0007829|PDB:3JTJ"
FT HELIX 189..195
FT /evidence="ECO:0007829|PDB:3JTJ"
FT HELIX 202..207
FT /evidence="ECO:0007829|PDB:3JTJ"
FT HELIX 212..216
FT /evidence="ECO:0007829|PDB:3JTJ"
FT STRAND 222..225
FT /evidence="ECO:0007829|PDB:3JTJ"
FT HELIX 237..247
FT /evidence="ECO:0007829|PDB:3JTJ"
SQ SEQUENCE 250 AA; 27336 MW; 5D29AF9F07CA4F08 CRC64;
MSFIAIIPAR YASTRLPGKP LADIAGKPMV VHVMERALAS GADRVIVATD HPDVVKAVEA
AGGEVCLTRA DHQSGTERLA EVIEHYGFAD DDIIVNVQGD EPLVPPVIIR QVADNLAACS
AGMATLAVPI ASSEEAFNPN AVKVVMDAQG YALYFSRATI PWERERFAQS KETIGDCFLR
HIGIYAYRAG FIRRYVNWAP SQLEQIELLE QLRVLWYGEK IHVAVAKAVP AVGVDTQSDL
DRVRAIMLNQ
