ID   KDSR_DICDI              Reviewed;         334 AA.
AC   Q556J2; Q86AN0;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 123.
DE   RecName: Full=3-ketodihydrosphingosine reductase;
DE            Short=KDS reductase;
DE            EC=1.1.1.102;
DE   AltName: Full=3-dehydrosphinganine reductase;
DE   Flags: Precursor;
GN   Name=ksrA-1; ORFNames=DDB_G0272883;
GN   and
GN   Name=ksrA-2; ORFNames=DDB_G0274015;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Catalyzes the reduction of 3-ketodihydrosphingosine (KDS) to
CC       dihydrosphingosine (DHS). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NADP(+) + sphinganine = 3-oxosphinganine + H(+) + NADPH;
CC         Xref=Rhea:RHEA:22640, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:57817, ChEBI:CHEBI:58299, ChEBI:CHEBI:58349;
CC         EC=1.1.1.102;
CC   -!- PATHWAY: Lipid metabolism; sphingolipid metabolism.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
CC   -!- CAUTION: The gene for this protein is duplicated in strains AX3 and
CC       AX4. These strains contain a duplication of a segment of 750 kb of
CC       chromosome 2 compared to the corresponding sequence in strain AX2.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000011; EAL70441.1; -; Genomic_DNA.
DR   EMBL; AAFI02000009; EAL71079.1; -; Genomic_DNA.
DR   RefSeq; XP_644366.1; XM_639274.1.
DR   RefSeq; XP_645048.1; XM_639956.1.
DR   AlphaFoldDB; Q556J2; -.
DR   SMR; Q556J2; -.
DR   STRING; 44689.DDB0232044; -.
DR   PaxDb; Q556J2; -.
DR   EnsemblProtists; EAL70441; EAL70441; DDB_G0274015.
DR   EnsemblProtists; EAL71079; EAL71079; DDB_G0272883.
DR   GeneID; 8618724; -.
DR   GeneID; 8619252; -.
DR   KEGG; ddi:DDB_G0272883; -.
DR   KEGG; ddi:DDB_G0274015; -.
DR   dictyBase; DDB_G0272883; ksrA-1.
DR   dictyBase; DDB_G0274015; ksrA-2.
DR   eggNOG; KOG1210; Eukaryota.
DR   HOGENOM; CLU_010194_3_2_1; -.
DR   InParanoid; Q556J2; -.
DR   OMA; NCAGMAI; -.
DR   PhylomeDB; Q556J2; -.
DR   Reactome; R-DDI-1660661; Sphingolipid de novo biosynthesis.
DR   UniPathway; UPA00222; -.
DR   PRO; PR:Q556J2; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0005811; C:lipid droplet; HDA:dictyBase.
DR   GO; GO:0047560; F:3-dehydrosphinganine reductase activity; ISS:dictyBase.
DR   GO; GO:0006666; P:3-keto-sphinganine metabolic process; IBA:GO_Central.
DR   GO; GO:0030148; P:sphingolipid biosynthetic process; IBA:GO_Central.
DR   CDD; cd08939; KDSR-like_SDR_c; 1.
DR   InterPro; IPR045022; KDSR-like.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   Lipid metabolism; NADP; Oxidoreductase; Reference proteome; Signal;
KW   Sphingolipid metabolism.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..334
FT                   /note="3-ketodihydrosphingosine reductase"
FT                   /id="PRO_0000331449"
SQ   SEQUENCE   334 AA;  36470 MW;  5543235D173D2C93 CRC64;
     MIIYILFSLL AAVIVHLVYK KQTGFKLKGK HIVVVGGSSG IGKELVYELL KENIASISVI
     SRSLDKLRSV VDDCPSEVCT KSTPLNVGSL KSKIEIYSCD ITNKIKVKET IAQIVSKNQG
     GKIDCLINCA GFAIPGYFIE QDEEIFEKTM QLDYFGSLYA TKEVVPLMIE NGGQGGHIVF
     VSSTCGLVGV PGYSTYCPSK FALRGLAETL RSELKPYKIT FSVVYPPDTD TPGYQQENLT
     KPEETVAISG GGKAVSPLEV AKSIVSGIKN GDYHIAYDVP TKLCAVLSPG LTPFYFSFFD
     ILLAPICRLV GIIAMNQNDA EVLKSWKKRN QLKK