KEA4_ARATH
ID KEA4_ARATH Reviewed; 592 AA.
AC Q9ZUN3;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 15-JUN-2010, sequence version 2.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=K(+) efflux antiporter 4 {ECO:0000303|PubMed:11500563};
DE Short=AtKEA4 {ECO:0000303|PubMed:11500563};
DE Flags: Precursor;
GN Name=KEA4 {ECO:0000303|PubMed:11500563}; OrderedLocusNames=At2g19600;
GN ORFNames=F3P11.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
RN [5]
RP FUNCTION, GENE FAMILY, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=24278440; DOI=10.1371/journal.pone.0081463;
RA Zheng S., Pan T., Fan L., Qiu Q.S.;
RT "A novel AtKEA gene family, homolog of bacterial K+/H+ antiporters, plays
RT potential roles in K+ homeostasis and osmotic adjustment in Arabidopsis.";
RL PLoS ONE 8:E81463-E81463(2013).
CC -!- FUNCTION: K(+)/H(+) antiporter involved in K(+) homeostasis and osmotic
CC adjustment. {ECO:0000269|PubMed:24278440}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9ZUN3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9ZUN3-2; Sequence=VSP_039363;
CC -!- TISSUE SPECIFICITY: Expressed in shoots and roots.
CC {ECO:0000269|PubMed:24278440}.
CC -!- INDUCTION: Up-regulated by low K(+) stress and down-regulated by high
CC K(+). {ECO:0000269|PubMed:24278440}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 2 (CPA2)
CC transporter (TC 2.A.37) family. KEA (TC 2.A.37.1) subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD10158.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC005917; AAD10158.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC06899.1; -; Genomic_DNA.
DR EMBL; AY091119; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; G84578; G84578.
DR RefSeq; NP_849990.1; NM_179659.2. [Q9ZUN3-1]
DR AlphaFoldDB; Q9ZUN3; -.
DR SMR; Q9ZUN3; -.
DR STRING; 3702.AT2G19600.1; -.
DR iPTMnet; Q9ZUN3; -.
DR PaxDb; Q9ZUN3; -.
DR PRIDE; Q9ZUN3; -.
DR ProteomicsDB; 238212; -. [Q9ZUN3-1]
DR EnsemblPlants; AT2G19600.1; AT2G19600.1; AT2G19600. [Q9ZUN3-1]
DR GeneID; 816479; -.
DR Gramene; AT2G19600.1; AT2G19600.1; AT2G19600. [Q9ZUN3-1]
DR KEGG; ath:AT2G19600; -.
DR Araport; AT2G19600; -.
DR TAIR; locus:2050382; AT2G19600.
DR eggNOG; KOG1650; Eukaryota.
DR HOGENOM; CLU_005126_11_1_1; -.
DR InParanoid; Q9ZUN3; -.
DR OMA; IKAHASK; -.
DR OrthoDB; 522572at2759; -.
DR PhylomeDB; Q9ZUN3; -.
DR PRO; PR:Q9ZUN3; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZUN3; baseline and differential.
DR Genevisible; Q9ZUN3; AT.
DR GO; GO:0000138; C:Golgi trans cisterna; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015386; F:potassium:proton antiporter activity; IEA:InterPro.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR045158; KEA4/5/6-like.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR PANTHER; PTHR16254; PTHR16254; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Antiport; Ion transport; Membrane; Potassium;
KW Potassium transport; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix; Transport.
FT SIGNAL 1..35
FT /evidence="ECO:0000255"
FT CHAIN 36..592
FT /note="K(+) efflux antiporter 4"
FT /evidence="ECO:0000255"
FT /id="PRO_0000395100"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 248..268
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 313..333
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 388..408
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 437..457
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 462..482
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 491..511
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 523..545
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..261
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_039363"
SQ SEQUENCE 592 AA; 64249 MW; 75708F72D18B38DA CRC64;
MRRCKNNTDK FSVITMRLLT LLLICTFFFF FSFAYSAESD NETDSVVTRE INGTVVESNA
TSAKPREDSF ADMIDRALEK EFPDNDQNEV PDPGSFNNSV ADQQAVLETV ARVKPKKNET
KTKEEKSFFN LDNENGVEDT PRLIDRKDNV FIMSNPKSKY PVLQLDLRLI SDLVVVIVSA
TCGGIAFACA GQPVITGYLL AGSIIGPGGL SFVSEMVQVE TVAQFGVIFL LFALGLEFSA
AKLRVVRAVA IPGGLLQIFL FMCLSGITAS LCGGKLTEGI FVGAFLSMSS TAVVLKFLME
RNSISALHGQ ITVGTLILQD CAVGLLFALL PVLGGTSGVL QGVLSMAKSL AILIAFLGAL
FVLSRTWVPW FLKLMTSLSS QTNELYQLAA VAFCLLVAWC SDKLGLSLEL GSFAAGVMIS
TTDLAQHTLE QVEPIRNFFA ALFLASIGML IHMHFLWNHV DILLAAVLLV IVIKTVVVAI
VVKVFGYNNK TAVLVGMSLA QIGEFAFVLL SRASNLHLIE SKLYLLLLGT TALSLVTTPL
LFKLIPAVVH LGVLLRWFSP DSSTEIGFKG ELYHSESAKR ISLMIQGSLH DS
