KEA5_ARATH
ID KEA5_ARATH Reviewed; 568 AA.
AC Q8VYR9; Q9FLI8;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=K(+) efflux antiporter 5 {ECO:0000303|PubMed:11500563};
DE Short=AtKEA5 {ECO:0000303|PubMed:11500563};
DE Flags: Precursor;
GN Name=KEA5 {ECO:0000303|PubMed:11500563};
GN OrderedLocusNames=At5g51710 {ECO:0000312|Araport:AT5G51710};
GN ORFNames=MIO24.16 {ECO:0000312|EMBL:BAB11240.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
RN [6]
RP FUNCTION, GENE FAMILY, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=24278440; DOI=10.1371/journal.pone.0081463;
RA Zheng S., Pan T., Fan L., Qiu Q.S.;
RT "A novel AtKEA gene family, homolog of bacterial K+/H+ antiporters, plays
RT potential roles in K+ homeostasis and osmotic adjustment in Arabidopsis.";
RL PLoS ONE 8:E81463-E81463(2013).
CC -!- FUNCTION: K(+)/H(+) antiporter involved in K(+) homeostasis and osmotic
CC adjustment. {ECO:0000269|PubMed:24278440}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8VYR9-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in shoots and roots.
CC {ECO:0000269|PubMed:24278440}.
CC -!- INDUCTION: Up-regulated by osmotic stress.
CC {ECO:0000269|PubMed:24278440}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 2 (CPA2)
CC transporter (TC 2.A.37) family. KEA (TC 2.A.37.1) subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB11240.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB010074; BAB11240.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED96117.1; -; Genomic_DNA.
DR EMBL; AY070064; AAL49821.1; -; mRNA.
DR EMBL; AY084820; AAM61386.1; -; mRNA.
DR RefSeq; NP_568763.1; NM_124550.2. [Q8VYR9-1]
DR AlphaFoldDB; Q8VYR9; -.
DR SMR; Q8VYR9; -.
DR STRING; 3702.AT5G51710.1; -.
DR TCDB; 2.A.37.1.8; the monovalent cation:proton antiporter-2 (cpa2) family.
DR PaxDb; Q8VYR9; -.
DR PRIDE; Q8VYR9; -.
DR ProteomicsDB; 237043; -. [Q8VYR9-1]
DR EnsemblPlants; AT5G51710.1; AT5G51710.1; AT5G51710. [Q8VYR9-1]
DR GeneID; 835245; -.
DR Gramene; AT5G51710.1; AT5G51710.1; AT5G51710. [Q8VYR9-1]
DR KEGG; ath:AT5G51710; -.
DR Araport; AT5G51710; -.
DR TAIR; locus:2165326; AT5G51710.
DR eggNOG; KOG1650; Eukaryota.
DR InParanoid; Q8VYR9; -.
DR PhylomeDB; Q8VYR9; -.
DR PRO; PR:Q8VYR9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8VYR9; baseline and differential.
DR Genevisible; Q8VYR9; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015386; F:potassium:proton antiporter activity; IEA:InterPro.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR045158; KEA4/5/6-like.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR PANTHER; PTHR16254; PTHR16254; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Antiport; Ion transport; Membrane; Potassium;
KW Potassium transport; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix; Transport.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..568
FT /note="K(+) efflux antiporter 5"
FT /evidence="ECO:0000255"
FT /id="PRO_0000395101"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 334..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 389..409
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 422..442
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 447..467
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 476..496
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 510..530
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 59..78
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 568 AA; 61598 MW; 76D0C1B04DA3D613 CRC64;
MARFAVIGLT FLLLLGTSLS ARSDEETRER FYGNVVNSTA PGNGEGSIAK MFDRVLEKEF
SENDSPEGSD GASFNSSVAD QQAEIETVAK VTHEKGKRND TQENNGTRPF QLQDVFSLEN
EDSDDMTLID KKNNVFVMSN KKSKYPILQV DLRLISDLVV IIVFAAIGGI VFSCLGQPVI
VGYLLAGSII GPGGLKFISE MVQVETVAQF GVVFLLFALG LEFSMTKLKV VGPVAVLGGL
LQIVLLMFLC GVTALLCGAR LSEGIFVGAF LSMSSTAVVV KFLVERNSTS SLHGQVTIGI
LIFQDCVVGL LFALLPVLGG NSGLLQGIIS MGKLLLILSI YLTVASLLTW SFVPRFLKLM
IQLSSQTNEL YQLAAVAFCL LSAWCSDKLG LSLELGSFVA GVMLSTTEFA QHTLEQVEPI
RNLFAALFLS SIGMLINVHF LWNHVDILLA SVILVIVIKT AIAAVVVKAF RYNMRISFHV
GVLLAQIGEF AFVLLSRASN LHVIEGKMYL LLLGTTALSL VTTPLLFKLI PSAMNLGVLL
RWFPSENSSP NESLQEKASL IEVHNRTK
