KEBAB_DROME
ID KEBAB_DROME Reviewed; 548 AA.
AC F0JAI6; Q961C7; Q9VQ69;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 1.
DT 03-AUG-2022, entry version 59.
DE RecName: Full=Kinetochore and Eb1-associated basic protein {ECO:0000303|PubMed:21912673};
GN Name=Kebab {ECO:0000303|PubMed:21912673};
GN ORFNames=CG31672 {ECO:0000312|FlyBase:FBgn0028952};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:ADY17727.1};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAK93101.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAK93101.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAK93101.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000312|EMBL:ADY17727.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC STRAIN=Berkeley {ECO:0000312|EMBL:ADY17727.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:ADY17727.1};
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP INTERACTION WITH EB1, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND
RP MUTAGENESIS OF 151-ILE-PRO-152 AND 170-ILE-PRO-171.
RX PubMed=21912673; DOI=10.1371/journal.pone.0024174;
RA Meireles A.M., Dzhindzhev N.S., Ohkura H.;
RT "Kebab: kinetochore and EB1 associated basic protein that dynamically
RT changes its localisation during Drosophila mitosis.";
RL PLoS ONE 6:E24174-E24174(2011).
CC -!- SUBUNIT: Interacts with Eb1 via the two SxIP motifs; the interaction is
CC not required for kebab kinetochore localization.
CC {ECO:0000269|PubMed:21912673}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:21912673}.
CC Cytoplasm, perinuclear region {ECO:0000269|PubMed:21912673}.
CC Chromosome, centromere, kinetochore {ECO:0000269|PubMed:21912673}.
CC Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:21912673}.
CC Note=During metaphase expressed in the kinetochores. During anaphase
CC expression in the kinetochores progressively increases, and at late
CC anaphase it is also expressed in the microtubules, specifically in the
CC central spindle and centrosomal region. During telophase expression
CC increases in the microtubules, and it is associated with residual
CC spindle microtubules between chromosomes that have separated. At
CC interphase it is expressed in the cytoplasm particularly around the
CC nucleus. {ECO:0000269|PubMed:21912673}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B {ECO:0000312|FlyBase:FBgn0028952};
CC IsoId=F0JAI6-1; Sequence=Displayed;
CC Name=A {ECO:0000312|FlyBase:FBgn0028952};
CC IsoId=F0JAI6-2; Sequence=VSP_058659;
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Flies are viable and
CC fertile. RNAi-mediated knockdown does not affect mitotic progression.
CC {ECO:0000269|PubMed:21912673}.
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DR EMBL; AE014134; AAF51310.2; -; Genomic_DNA.
DR EMBL; AE014134; AGB92437.1; -; Genomic_DNA.
DR EMBL; AY051677; AAK93101.1; -; mRNA.
DR EMBL; BT126028; ADY17727.1; -; mRNA.
DR RefSeq; NP_001259900.1; NM_001272971.1. [F0JAI6-1]
DR RefSeq; NP_722756.1; NM_164451.2. [F0JAI6-2]
DR AlphaFoldDB; F0JAI6; -.
DR SMR; F0JAI6; -.
DR IntAct; F0JAI6; 1.
DR PaxDb; F0JAI6; -.
DR DNASU; 48440; -.
DR EnsemblMetazoa; FBtr0077825; FBpp0077498; FBgn0028952. [F0JAI6-2]
DR EnsemblMetazoa; FBtr0310547; FBpp0302684; FBgn0028952. [F0JAI6-1]
DR GeneID; 48440; -.
DR KEGG; dme:Dmel_CG31672; -.
DR UCSC; CG31672-RA; d. melanogaster.
DR CTD; 48440; -.
DR FlyBase; FBgn0028952; Kebab.
DR VEuPathDB; VectorBase:FBgn0028952; -.
DR OMA; PIRPKVI; -.
DR BioGRID-ORCS; 48440; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 48440; -.
DR PRO; PR:F0JAI6; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0028952; Expressed in eye disc (Drosophila) and 15 other tissues.
DR ExpressionAtlas; F0JAI6; baseline and differential.
DR GO; GO:0000776; C:kinetochore; IDA:FlyBase.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:FlyBase.
DR GO; GO:0005876; C:spindle microtubule; IDA:FlyBase.
DR GO; GO:0043515; F:kinetochore binding; IDA:FlyBase.
DR GO; GO:0008017; F:microtubule binding; IDA:FlyBase.
DR Gene3D; 1.10.418.30; -; 1.
DR InterPro; IPR038273; Ndc80_sf.
PE 1: Evidence at protein level;
KW Alternative splicing; Centromere; Chromosome; Coiled coil; Cytoplasm;
KW Cytoskeleton; Kinetochore; Reference proteome.
FT CHAIN 1..548
FT /note="Kinetochore and Eb1-associated basic protein"
FT /id="PRO_0000438398"
FT REGION 1..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 82..181
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 100..253
FT /note="Important for kinetochore and microtubule
FT localization"
FT /evidence="ECO:0000269|PubMed:21912673"
FT REGION 237..372
FT /note="CH (calponin-homology)-like region, which is not
FT required for kinetochore and microtubule localization"
FT /evidence="ECO:0000269|PubMed:21912673"
FT COILED 386..457
FT /evidence="ECO:0000255"
FT MOTIF 149..152
FT /note="SXIP motif 1"
FT /evidence="ECO:0000269|PubMed:21912673"
FT MOTIF 168..171
FT /note="SXIP motif 2"
FT /evidence="ECO:0000269|PubMed:21912673"
FT COMPBIAS 82..107
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 124..144
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 146..173
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 116
FT /note="Missing (in isoform A)"
FT /id="VSP_058659"
FT MUTAGEN 151..152
FT /note="IP->NT: Slight reduction in Eb1 binding. No effect
FT on kinetochore and microtubule localization."
FT /evidence="ECO:0000269|PubMed:21912673"
FT MUTAGEN 170..171
FT /note="IP->RT: No effect on Eb1 binding, and no effect on
FT kinetochore and microtubule localization."
FT /evidence="ECO:0000269|PubMed:21912673"
SQ SEQUENCE 548 AA; 63527 MW; F3F0A2601C6B7B57 CRC64;
MSSMAKSPDM RTPGCCSPLR TKELLERQRS SRCTPAKGYL TPRNCQSPKH PEMRIPSIFV
TDADYGLERP KQLLQRLERS LYRSSSASKV PPKHSLLASQ NRQRTWEGPK TPEFRSRTNK
TIPASEPRPR RAKELLEDLR SKHQGTPATK IPSQRNPKEN QELSKSHTCI PSSEPQPIRP
KLILERERQE SITNRLASTS IDRLKTKPPR SSFTSSRLLV PQMGFSYPKD PKRLHESDKG
IKLTTSKRKL DFKTELGTDW LRRELEKIGK EWRKKTDYQL RQLISGFVKQ LVRLLPFNGI
TFSHLSRDCY VQQMVEALQQ LQYTKKVNKS WLQTPNSTQA IAHVLELLNF LLDVLEHRKG
EGMCALPVVS EKQRIEQLAS ASGTSYDVMS LQQKFENIKI EKERLNNYQE SLMPESPVSK
DMDKVTERDG NQDFVRLLDF QKETLHELQL QRLRLQEFSE LVSLAKIKLK RCCKANKQCI
EAFNDQIQDL ADCVVLRNRN IGLLTQLHLN DNPKEEELHE RMKQLQRLYE DNYSNLLQLN
IKPPQGSP
