ID   KEFB_ALKAM              Reviewed;         661 AA.
AC   Q0ZAH7;
DT   19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Putative K(+) efflux antiporter KefB;
GN   Name=kefB; Synonyms=napA;
OS   Alkalimonas amylolytica.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alkalimonas.
OX   NCBI_TaxID=152573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND GENE NAME.
RC   STRAIN=N10;
RX   PubMed=17600061; DOI=10.1099/mic.0.2007/007450-0;
RA   Wei Y., Liu J., Ma Y., Krulwich T.A.;
RT   "Three putative cation/proton antiporters from the soda lake alkaliphile
RT   Alkalimonas amylolytica N10 complement an alkali-sensitive Escherichia coli
RT   mutant.";
RL   Microbiology 153:2168-2179(2007).
CC   -!- FUNCTION: May operate as a K(+)/H(+) antiporter.
CC       {ECO:0000269|PubMed:17600061}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 2 (CPA2)
CC       transporter (TC 2.A.37) family. KEA (TC 2.A.37.1) subfamily.
CC       {ECO:0000305}.
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DR   EMBL; DQ649019; ABG37986.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q0ZAH7; -.
DR   SMR; Q0ZAH7; -.
DR   STRING; 152573.SAMN04488051_10349; -.
DR   TCDB; 2.A.37.1.3; the monovalent cation:proton antiporter-2 (cpa2) family.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008324; F:cation transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0015299; F:solute:proton antiporter activity; IEA:InterPro.
DR   GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1530.20; -; 1.
DR   Gene3D; 3.30.70.1450; -; 1.
DR   InterPro; IPR006153; Cation/H_exchanger.
DR   InterPro; IPR038770; Na+/solute_symporter_sf.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR006037; RCK_C.
DR   InterPro; IPR036721; RCK_C_sf.
DR   InterPro; IPR003148; RCK_N.
DR   Pfam; PF00999; Na_H_Exchanger; 1.
DR   Pfam; PF02080; TrkA_C; 1.
DR   Pfam; PF02254; TrkA_N; 1.
DR   SUPFAM; SSF116726; SSF116726; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS51202; RCK_C; 1.
DR   PROSITE; PS51201; RCK_N; 1.
PE   3: Inferred from homology;
KW   Antiport; Cell membrane; Ion transport; Membrane; Potassium;
KW   Potassium transport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..661
FT                   /note="Putative K(+) efflux antiporter KefB"
FT                   /id="PRO_0000425722"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        31..51
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        154..174
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        178..198
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        224..244
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        273..293
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        296..316
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        330..350
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        360..380
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          412..534
FT                   /note="RCK N-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00543"
FT   DOMAIN          571..656
FT                   /note="RCK C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00544"
SQ   SEQUENCE   661 AA;  71766 MW;  D11E8222F2AA8B2E CRC64;
     MLLDGTLIQL LFLLGFMVFM VMLFQRAHIP ASIAYLLVGV LLGAHTAGPV ISEGYIHKIA
     EFGIVFLLFT IGLRFSWQQI YQLRHTILGL GTAQVGLTTL LVALLLWAMG VASVVAFVIG
     AVFAQSSTTI ISKQLLEQGE DQSRHGRLGI SLSVFQDITA VPFIIVIPVL GVAMAQDIAS
     TLGMALFKAV LATALVVLVG RYLLRHLFHR VSSSDSAELF TLTVLLVCLA AAWLTQSLGL
     SMAFGAFLAG MVMGETEFKL QVEAAIRPFR DVLLGIFFVS IGMLLDPMLL PEIGHIALAG
     ALLLLLIKIV LVTALVLATG VALETAFRTG LILAVGGEFG FALLALALEG GTLDSRSSQI
     ILTSVLLSMM LAVFLIRYNL QLSRFVVGRW IGQAAVSSEP FDDIEQHGLQ QHVVIAGFGR
     IGQGVAQFLQ KEQVPYIGLD LDAARVKNAR LADVPVFYAD STDPDTLIAV GLAKARLLVI
     SHEDLSAALT TLRHARTLHP DLPVIVRTRD ESHVAELRQA GATEVIPETI EAGMMLTSHV
     LLMLNVPARR VNQLVQEQRI NRYQLLRQQF RGSADLLKTQ QQEQLKAVLL AEGSPAIDQS
     LQMLDLAQYG IQLTELVRAH NRIEQPSLDM VLQENDVLVL FGSAEALEQA SLVLTHRLAA
     D