ID   KEFB_SHIB3              Reviewed;         601 AA.
AC   B2U3F5;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Glutathione-regulated potassium-efflux system protein KefB {ECO:0000255|HAMAP-Rule:MF_01412};
DE   AltName: Full=K(+)/H(+) antiporter {ECO:0000255|HAMAP-Rule:MF_01412};
GN   Name=kefB {ECO:0000255|HAMAP-Rule:MF_01412};
GN   OrderedLocusNames=SbBS512_E3725;
OS   Shigella boydii serotype 18 (strain CDC 3083-94 / BS512).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=344609;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 3083-94 / BS512;
RA   Rasko D.A., Rosovitz M., Maurelli A.T., Myers G., Seshadri R., Cer R.,
RA   Jiang L., Ravel J., Sebastian Y.;
RT   "Complete sequence of Shigella boydii serotype 18 strain BS512.";
RL   Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Pore-forming subunit of a potassium efflux system that
CC       confers protection against electrophiles. Catalyzes K(+)/H(+) antiport.
CC       {ECO:0000255|HAMAP-Rule:MF_01412}.
CC   -!- SUBUNIT: Interacts with the regulatory subunit KefG.
CC       {ECO:0000255|HAMAP-Rule:MF_01412}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01412}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01412}.
CC   -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 2 (CPA2)
CC       transporter (TC 2.A.37) family. KefB subfamily. {ECO:0000255|HAMAP-
CC       Rule:MF_01412}.
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DR   EMBL; CP001063; ACD10229.1; -; Genomic_DNA.
DR   RefSeq; WP_000399107.1; NC_010658.1.
DR   AlphaFoldDB; B2U3F5; -.
DR   SMR; B2U3F5; -.
DR   STRING; 344609.SbBS512_E3725; -.
DR   EnsemblBacteria; ACD10229; ACD10229; SbBS512_E3725.
DR   KEGG; sbc:SbBS512_E3725; -.
DR   HOGENOM; CLU_005126_9_3_6; -.
DR   OMA; IRPFHDV; -.
DR   Proteomes; UP000001030; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0015503; F:glutathione-regulated potassium exporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015299; F:solute:proton antiporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1530.20; -; 1.
DR   HAMAP; MF_01412; K_H_efflux_KefB; 1.
DR   InterPro; IPR006153; Cation/H_exchanger.
DR   InterPro; IPR004771; K/H_exchanger.
DR   InterPro; IPR020884; K_H_efflux_KefB.
DR   InterPro; IPR038770; Na+/solute_symporter_sf.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR003148; RCK_N.
DR   Pfam; PF00999; Na_H_Exchanger; 1.
DR   Pfam; PF02254; TrkA_N; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR00932; 2a37; 1.
DR   PROSITE; PS51201; RCK_N; 1.
PE   3: Inferred from homology;
KW   Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Potassium; Potassium transport; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..601
FT                   /note="Glutathione-regulated potassium-efflux system
FT                   protein KefB"
FT                   /id="PRO_1000145532"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01412"
FT   TRANSMEM        29..49
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01412"
FT   TRANSMEM        55..75
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01412"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01412"
FT   TRANSMEM        115..135
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01412"
FT   TRANSMEM        152..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01412"
FT   TRANSMEM        177..197
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01412"
FT   TRANSMEM        207..227
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01412"
FT   TRANSMEM        230..250
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01412"
FT   TRANSMEM        262..282
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01412"
FT   TRANSMEM        284..304
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01412"
FT   TRANSMEM        324..344
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01412"
FT   TRANSMEM        356..376
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01412"
FT   DOMAIN          402..524
FT                   /note="RCK N-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01412"
SQ   SEQUENCE   601 AA;  66437 MW;  583C154256B9EBC2 CRC64;
     MEGSDFLLAG VLFLFAAVAA VPLASRLGIG AVLGYLLAGI AIGPWGLGFI SDVDEILHFS
     ELGVVFLMFI IGLELNPSKL WQLRRSIFGV GAAQVLLSAA LLAGLLMLTD FAWQAAVVGG
     IGLAMSSTAM ALQLMREKGM NRSESGQLGF SVLLFQDLAV IPALALVPLL AGSADEHFDW
     MKIGMKVLAF VGMLIGGRYL LRPVFRFIAA SGVREVFTAA TLLLVLGSAL FMDALGLSMA
     LGTFIAGVLL AESEYRHELE TAIDPFKGLL LGLFFISVGM SLNLGVLYIH LLWVVISVVV
     LVAVKILVLY LLARLYGVRS SERMQFAGVL SQGGEFAFVL FSTASSQRLF QGDQMALLLV
     TVTLSMMTTP LLMKLVDKWL SRQFNGPEEE DEKPWVNDDK PQVIVVGFGR FGQVIGRLLM
     ANKMRITVLE RDISAVNLMR KYGYKVYYGD ATQVDLLRSA GAEAAESIVI TCNEPEDTMK
     LVEICQQHFP HLHILARARG RVEAHELLQA GVTQFSRETF SSALELGRKT LVTLGMHPHQ
     AQRAQLHFRR LDMRMLRELI PMHADTVQIS RAREARRELE EIFQREMQQE RRQLDGWDEF
     E