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KEFG_ECOLI
ID   KEFG_ECOLI              Reviewed;         184 AA.
AC   P0A756; P45534; Q2M717;
DT   07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Glutathione-regulated potassium-efflux system ancillary protein KefG {ECO:0000255|HAMAP-Rule:MF_01415};
DE   AltName: Full=Putative quinone oxidoreductase KefG {ECO:0000255|HAMAP-Rule:MF_01415};
DE            EC=1.6.5.2 {ECO:0000255|HAMAP-Rule:MF_01415};
GN   Name=kefG {ECO:0000255|HAMAP-Rule:MF_01415}; Synonyms=yheR;
GN   OrderedLocusNames=b3351, JW3314;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   FUNCTION.
RC   STRAIN=Frag5;
RX   PubMed=11053405; DOI=10.1128/jb.182.22.6536-6540.2000;
RA   Miller S., Ness L.S., Wood C.M., Fox B.C., Booth I.R.;
RT   "Identification of an ancillary protein, YabF, required for activity of the
RT   KefC glutathione-gated potassium efflux system in Escherichia coli.";
RL   J. Bacteriol. 182:6536-6540(2000).
CC   -!- FUNCTION: Regulatory subunit of a potassium efflux system that confers
CC       protection against electrophiles. Required for full activity of KefB.
CC       {ECO:0000255|HAMAP-Rule:MF_01415, ECO:0000269|PubMed:11053405}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a quinone + H(+) + NADH = a quinol + NAD(+);
CC         Xref=Rhea:RHEA:46160, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124; EC=1.6.5.2;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01415};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a quinone + H(+) + NADPH = a quinol + NADP(+);
CC         Xref=Rhea:RHEA:46164, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:132124; EC=1.6.5.2;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01415};
CC   -!- SUBUNIT: Interacts with KefB. {ECO:0000255|HAMAP-Rule:MF_01415}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01415}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01415}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_01415}.
CC   -!- SIMILARITY: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefG
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01415}.
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DR   EMBL; U18997; AAA58148.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76376.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77939.1; -; Genomic_DNA.
DR   PIR; B65129; B65129.
DR   RefSeq; NP_417810.1; NC_000913.3.
DR   AlphaFoldDB; P0A756; -.
DR   SMR; P0A756; -.
DR   BioGRID; 4263025; 13.
DR   BioGRID; 852168; 1.
DR   ComplexPortal; CPX-3090; Glutathione-regulated potassium-efflux system KefB-KefG complex.
DR   IntAct; P0A756; 6.
DR   STRING; 511145.b3351; -.
DR   TCDB; 2.A.37.1.2; the monovalent cation:proton antiporter-2 (cpa2) family.
DR   jPOST; P0A756; -.
DR   PaxDb; P0A756; -.
DR   PRIDE; P0A756; -.
DR   EnsemblBacteria; AAC76376; AAC76376; b3351.
DR   EnsemblBacteria; BAE77939; BAE77939; BAE77939.
DR   GeneID; 947857; -.
DR   KEGG; ecj:JW3314; -.
DR   KEGG; eco:b3351; -.
DR   PATRIC; fig|511145.12.peg.3445; -.
DR   EchoBASE; EB2739; -.
DR   eggNOG; COG2249; Bacteria.
DR   HOGENOM; CLU_058643_0_1_6; -.
DR   InParanoid; P0A756; -.
DR   OMA; RYPMSDI; -.
DR   PhylomeDB; P0A756; -.
DR   BioCyc; EcoCyc:G7717-MON; -.
DR   PRO; PR:P0A756; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:1903103; C:potassium:proton antiporter complex; IC:ComplexPortal.
DR   GO; GO:0009055; F:electron transfer activity; IBA:GO_Central.
DR   GO; GO:0010181; F:FMN binding; IBA:GO_Central.
DR   GO; GO:0003955; F:NAD(P)H dehydrogenase (quinone) activity; IBA:GO_Central.
DR   GO; GO:0050136; F:NADH dehydrogenase (quinone) activity; IEA:RHEA.
DR   GO; GO:0008753; F:NADPH dehydrogenase (quinone) activity; IEA:RHEA.
DR   GO; GO:0032414; P:positive regulation of ion transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:1901381; P:positive regulation of potassium ion transmembrane transport; IMP:EcoCyc.
DR   GO; GO:0006813; P:potassium ion transport; IEA:InterPro.
DR   GO; GO:0051453; P:regulation of intracellular pH; IC:ComplexPortal.
DR   Gene3D; 3.40.50.360; -; 1.
DR   HAMAP; MF_01415; K_H_efflux_KefG; 1.
DR   InterPro; IPR003680; Flavodoxin_fold.
DR   InterPro; IPR029039; Flavoprotein-like_sf.
DR   InterPro; IPR023947; K_H_efflux_KefG.
DR   Pfam; PF02525; Flavodoxin_2; 1.
DR   SUPFAM; SSF52218; SSF52218; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; NAD; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN           1..184
FT                   /note="Glutathione-regulated potassium-efflux system
FT                   ancillary protein KefG"
FT                   /id="PRO_0000071642"
SQ   SEQUENCE   184 AA;  20958 MW;  F3B1123F7378EAB7 CRC64;
     MMSQPAKVLL LYAHPESQDS VANRVLLKPA TQLSNVTVHD LYAHYPDFFI DIPREQALLR
     EHEVIVFQHP LYTYSCPALL KEWLDRVLSR GFASGPGGNQ LAGKYWRSVI TTGEPESAYR
     YDALNRYPMS DVLRPFELAA GMCRMHWLSP IIIYWARRQS AQELASHARA YGDWLANPLS
     PGGR
 
 
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