KEFG_SHIFL
ID KEFG_SHIFL Reviewed; 183 AA.
AC Q83PX9;
DT 03-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2003, sequence version 2.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=Glutathione-regulated potassium-efflux system ancillary protein KefG {ECO:0000255|HAMAP-Rule:MF_01415};
DE AltName: Full=Putative quinone oxidoreductase KefG {ECO:0000255|HAMAP-Rule:MF_01415};
DE EC=1.6.5.2 {ECO:0000255|HAMAP-Rule:MF_01415};
GN Name=kefG {ECO:0000255|HAMAP-Rule:MF_01415};
GN OrderedLocusNames=SF3370, S4393;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: Regulatory subunit of a potassium efflux system that confers
CC protection against electrophiles. Required for full activity of KefB.
CC {ECO:0000255|HAMAP-Rule:MF_01415}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinone + H(+) + NADH = a quinol + NAD(+);
CC Xref=Rhea:RHEA:46160, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124; EC=1.6.5.2;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01415};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinone + H(+) + NADPH = a quinol + NADP(+);
CC Xref=Rhea:RHEA:46164, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:132124; EC=1.6.5.2;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01415};
CC -!- SUBUNIT: Interacts with KefB. {ECO:0000255|HAMAP-Rule:MF_01415}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01415}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01415}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_01415}.
CC -!- SIMILARITY: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefG
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01415}.
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DR EMBL; AE005674; AAN44833.2; -; Genomic_DNA.
DR EMBL; AE014073; AAP19345.1; -; Genomic_DNA.
DR RefSeq; NP_709126.2; NC_004337.2.
DR RefSeq; WP_001445921.1; NZ_WPGW01000003.1.
DR AlphaFoldDB; Q83PX9; -.
DR SMR; Q83PX9; -.
DR STRING; 198214.SF3370; -.
DR EnsemblBacteria; AAN44833; AAN44833; SF3370.
DR EnsemblBacteria; AAP19345; AAP19345; S4393.
DR GeneID; 1026554; -.
DR KEGG; sfl:SF3370; -.
DR KEGG; sfx:S4393; -.
DR PATRIC; fig|198214.7.peg.3980; -.
DR HOGENOM; CLU_058643_0_1_6; -.
DR OMA; RYPMSDI; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0050136; F:NADH dehydrogenase (quinone) activity; IEA:RHEA.
DR GO; GO:0008753; F:NADPH dehydrogenase (quinone) activity; IEA:RHEA.
DR GO; GO:0032414; P:positive regulation of ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:1901381; P:positive regulation of potassium ion transmembrane transport; IEA:UniProtKB-UniRule.
DR GO; GO:0006813; P:potassium ion transport; IEA:InterPro.
DR Gene3D; 3.40.50.360; -; 1.
DR HAMAP; MF_01415; K_H_efflux_KefG; 1.
DR InterPro; IPR003680; Flavodoxin_fold.
DR InterPro; IPR029039; Flavoprotein-like_sf.
DR InterPro; IPR023947; K_H_efflux_KefG.
DR Pfam; PF02525; Flavodoxin_2; 1.
DR SUPFAM; SSF52218; SSF52218; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; NAD; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..183
FT /note="Glutathione-regulated potassium-efflux system
FT ancillary protein KefG"
FT /id="PRO_0000071649"
SQ SEQUENCE 183 AA; 20854 MW; FB70B4E4D44B9E43 CRC64;
MSQPAKVLLL YAHPESQDSV ANRVLLKPAT QLSNVTVHDL YAHYPDFFID IPREQALLRE
HEVIVFQHPL YTYSCPALLK EWLDRVLSRG FASGPGGNQL AGKYWRNVIT TGEPESAYRY
DALNRYPMSD VLRPFELAAG MCRMHWLSPI IIYWARRQSA KELASHARAY GDWLANPLSP
GGR
