KEG1_YEAST
ID KEG1_YEAST Reviewed; 200 AA.
AC P43614; A5A8M3; D6VTS5;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Beta-1,6-glucan synthesis-associated protein KEG1;
DE AltName: Full=KRE6-binding ER protein responsible for glucan synthesis protein 1;
GN Name=KEG1; OrderedLocusNames=YFR042W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE NAME, FUNCTION, INTERACTION WITH
RP KRE6, SUBCELLULAR LOCATION, VARIANTS VAL-3; CYS-55 AND ASP-84, AND
RP MUTAGENESIS OF HIS-124.
RC STRAIN=KA31a;
RX PubMed=17893149; DOI=10.1074/jbc.m706486200;
RA Nakamata K., Kurita T., Bhuiyan M.S.A., Sato K., Noda Y., Yoda K.;
RT "KEG1/YFR042w encodes a novel Kre6-binding endoplasmic reticulum membrane
RT protein responsible for beta-1,6-glucan synthesis in Saccharomyces
RT cerevisiae.";
RL J. Biol. Chem. 282:34315-34324(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204511 / S288c / AB972;
RX PubMed=8686379;
RX DOI=10.1002/(sici)1097-0061(199602)12:2<149::aid-yea893>3.0.co;2-g;
RA Eki T., Naitou M., Hagiwara H., Ozawa M., Sasanuma S., Sasanuma M.,
RA Tsuchiya Y., Shibata T., Hanaoka F., Murakami Y.;
RT "Analysis of a 36.2 kb DNA sequence including the right telomere of
RT chromosome VI from Saccharomyces cerevisiae.";
RL Yeast 12:149-167(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7670463; DOI=10.1038/ng0795-261;
RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA Eki T.;
RT "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT cerevisiae.";
RL Nat. Genet. 10:261-268(1995).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [6]
RP FUNCTION.
RX PubMed=18439903; DOI=10.1016/j.molcel.2008.02.021;
RA Ben-Aroya S., Coombes C., Kwok T., O'Donnell K.A., Boeke J.D., Hieter P.;
RT "Toward a comprehensive temperature-sensitive mutant repository of the
RT essential genes of Saccharomyces cerevisiae.";
RL Mol. Cell 30:248-258(2008).
CC -!- FUNCTION: Involved in the biosynthesis of (1->6)-beta-D-glucan polymers
CC of the cell wall. Required for viability. Involved in maintaining
CC chromosome stability. {ECO:0000269|PubMed:17893149,
CC ECO:0000269|PubMed:18439903}.
CC -!- SUBUNIT: Interacts with KRE6. {ECO:0000269|PubMed:17893149}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:17893149}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:17893149}.
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DR EMBL; AB303676; BAF62429.1; -; Genomic_DNA.
DR EMBL; D50617; BAA09281.1; -; Genomic_DNA.
DR EMBL; BK006940; DAA12485.1; -; Genomic_DNA.
DR PIR; S56297; S56297.
DR RefSeq; NP_116700.1; NM_001180007.1.
DR AlphaFoldDB; P43614; -.
DR BioGRID; 31200; 235.
DR DIP; DIP-1849N; -.
DR IntAct; P43614; 5.
DR MINT; P43614; -.
DR STRING; 4932.YFR042W; -.
DR PaxDb; P43614; -.
DR PRIDE; P43614; -.
DR EnsemblFungi; YFR042W_mRNA; YFR042W; YFR042W.
DR GeneID; 850603; -.
DR KEGG; sce:YFR042W; -.
DR SGD; S000001938; KEG1.
DR VEuPathDB; FungiDB:YFR042W; -.
DR eggNOG; ENOG502RXXA; Eukaryota.
DR HOGENOM; CLU_1367193_0_0_1; -.
DR InParanoid; P43614; -.
DR OMA; HFYDDYE; -.
DR BioCyc; YEAST:G3O-30489-MON; -.
DR PRO; PR:P43614; -.
DR Proteomes; UP000002311; Chromosome VI.
DR RNAct; P43614; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IDA:SGD.
DR GO; GO:0006078; P:(1->6)-beta-D-glucan biosynthetic process; IMP:SGD.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0051276; P:chromosome organization; IMP:SGD.
PE 1: Evidence at protein level;
KW Cell wall biogenesis/degradation; Endoplasmic reticulum; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..200
FT /note="Beta-1,6-glucan synthesis-associated protein KEG1"
FT /id="PRO_0000202695"
FT TOPO_DOM 1..15
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..44
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 66..82
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 104..145
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 167..173
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 195..200
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VARIANT 3
FT /note="G -> V (in strain: KA31a)"
FT /evidence="ECO:0000269|PubMed:17893149"
FT VARIANT 55
FT /note="Y -> C (in strain: KA31a)"
FT /evidence="ECO:0000269|PubMed:17893149"
FT VARIANT 84
FT /note="N -> D (in strain: KA31a)"
FT /evidence="ECO:0000269|PubMed:17893149"
FT MUTAGEN 124
FT /note="H->L: In KEG1-1; temperature-sensitive. Increased
FT sensitivity to chitin-binding dye Calcofluor white and
FT zymolyase. Shows K1 killer toxin resistance, and reduced
FT content of the cell wall beta-1,6-glucan."
FT /evidence="ECO:0000269|PubMed:17893149"
SQ SEQUENCE 200 AA; 23555 MW; E471CE48A72C2A61 CRC64;
MAGIKLTHKL YQYYQLATSF LYAALLIRWL ILMPLVGSRF LPGGIHEFLI YLMFYSSIME
VIWLLRFHGF KYGLLSRTFL KDLNFIYLVS VIHFYDDYEH ALILKNASYS SFIISLSLSQ
AYCHWCKLFK RKGVKERTLV WKVNTFVTLP ILYLSEFALL LLNIQVKNYH STPTLDIINR
VVLLAYFPVL LTAYKKLLTK
