KEI1_YEAST
ID KEI1_YEAST Reviewed; 221 AA.
AC Q06346; D6VSZ8; Q06999;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Inositol phosphorylceramide synthase regulatory subunit KEI1;
DE Short=ICP synthase regulatory subunit KEI1;
DE AltName: Full=KEX2-cleavable protein essential for inositol phosphorylceramide synthesis;
DE Contains:
DE RecName: Full=KEI1N;
DE Contains:
DE RecName: Full=KEI1C;
DE Flags: Precursor;
GN Name=KEI1; OrderedLocusNames=YDR367W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 11-221.
RC STRAIN=M5;
RA Miosga T., Juhnke H., Sterkel C., Zimmermann F.K.;
RT "A new yeast gene with homology to the aldo-keto reductase protein
RT family.";
RL Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION OF INTRON.
RX PubMed=10734188; DOI=10.1093/nar/28.8.1700;
RA Davis C.A., Grate L., Spingola M., Ares M. Jr.;
RT "Test of intron predictions reveals novel splice sites, alternatively
RT spliced mRNAs and new introns in meiotically regulated genes of yeast.";
RL Nucleic Acids Res. 28:1700-1706(2000).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH AUR1; COP1 AND SEC21, AND
RP MUTAGENESIS OF PHE-103 AND ARG-135.
RX PubMed=19726565; DOI=10.1091/mbc.e09-03-0235;
RA Sato K., Noda Y., Yoda K.;
RT "Kei1: a novel subunit of inositolphosphorylceramide synthase, essential
RT for its enzyme activity and Golgi localization.";
RL Mol. Biol. Cell 20:4444-4457(2009).
CC -!- FUNCTION: Regulatory component of the inositol phosphorylceramide (ICP)
CC synthase which catalyzes the addition of a phosphorylinositol group
CC onto ceramide to form inositol phosphorylceramide, an essential step in
CC sphingolipid biosynthesis. Helps the medial Golgi localization of IPC
CC synthase in a COPI vesicle-dependent manner.
CC {ECO:0000269|PubMed:19726565}.
CC -!- SUBUNIT: Component of the inositol phosphorylceramide synthase complex
CC composed of at least AUR1 and KEI1. Interacts (via C-terminal region)
CC with COP1 and SEC21. Note=The interaction with AUR1 seems to occur with
CC the full-length protein before cleavage by KEX2 since both full-length
CC and short chains of KEI1 interact with AUR1.
CC {ECO:0000269|PubMed:19726565}.
CC -!- INTERACTION:
CC Q06346; P36107: AUR1; NbExp=2; IntAct=EBI-38431, EBI-2046036;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:19726565}; Multi-pass
CC membrane protein {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:19726565}.
CC -!- PTM: The precursor protein is cleaved into two polypeptide chains,
CC KEI1N and KEI1C. The cleavage is performed in the Golgi apparatus by
CC the KEX2 protease which recognizes residue Arg-135. Generation of KEX2
CC cleavage site may have been an accidental event in evolution without
CC specific advantages or disadvantages in IPC synthesis.
CC -!- MISCELLANEOUS: Present with 3420 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the KEI1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB64803.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAA56685.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U28373; AAB64803.1; ALT_SEQ; Genomic_DNA.
DR EMBL; X80642; CAA56685.1; ALT_FRAME; Genomic_DNA.
DR EMBL; BK006938; DAA12208.1; -; Genomic_DNA.
DR PIR; S70234; S70234.
DR RefSeq; NP_010655.1; NM_001180675.1.
DR AlphaFoldDB; Q06346; -.
DR BioGRID; 32426; 17.
DR ComplexPortal; CPX-2894; Inositol phosphorylceramide synthase complex.
DR DIP; DIP-8823N; -.
DR IntAct; Q06346; 6.
DR STRING; 4932.YDR367W; -.
DR MaxQB; Q06346; -.
DR PaxDb; Q06346; -.
DR PRIDE; Q06346; -.
DR EnsemblFungi; YDR367W_mRNA; YDR367W; YDR367W.
DR GeneID; 851973; -.
DR KEGG; sce:YDR367W; -.
DR SGD; S000002775; KEI1.
DR VEuPathDB; FungiDB:YDR367W; -.
DR eggNOG; ENOG502QT2Z; Eukaryota.
DR HOGENOM; CLU_103819_1_0_1; -.
DR InParanoid; Q06346; -.
DR OMA; SMYIYSI; -.
DR BioCyc; MetaCyc:G3O-29917-MON; -.
DR BioCyc; YEAST:G3O-29917-MON; -.
DR BRENDA; 2.7.1.227; 984.
DR PRO; PR:Q06346; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q06346; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005794; C:Golgi apparatus; HDA:SGD.
DR GO; GO:0070916; C:inositol phosphoceramide synthase complex; IPI:SGD.
DR GO; GO:0030173; C:integral component of Golgi membrane; IDA:SGD.
DR GO; GO:0070917; F:inositol phosphoceramide synthase regulator activity; IMP:SGD.
DR GO; GO:0006673; P:inositol phosphoceramide metabolic process; IDA:ComplexPortal.
DR GO; GO:0030148; P:sphingolipid biosynthetic process; IPI:ComplexPortal.
DR InterPro; IPR013862; Kei1.
DR PANTHER; PTHR28077; PTHR28077; 1.
DR Pfam; PF08552; Kei1; 1.
PE 1: Evidence at protein level;
KW Golgi apparatus; Lipid metabolism; Membrane; Reference proteome;
KW Sphingolipid metabolism; Transmembrane; Transmembrane helix.
FT CHAIN 1..221
FT /note="Inositol phosphorylceramide synthase regulatory
FT subunit KEI1"
FT /id="PRO_0000253846"
FT CHAIN 1..135
FT /note="KEI1N"
FT /id="PRO_0000409445"
FT CHAIN 136..221
FT /note="KEI1C"
FT /id="PRO_0000409446"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 176..221
FT /note="COPI vesicle-binding"
FT MUTAGEN 103
FT /note="F->I: Decreases ICP synthase activity."
FT /evidence="ECO:0000269|PubMed:19726565"
FT MUTAGEN 135
FT /note="R->S: Impairs cleavage by KEX2."
FT /evidence="ECO:0000269|PubMed:19726565"
SQ SEQUENCE 221 AA; 25484 MW; 4EC6A3FF541FC6F5 CRC64;
MRSSLLTLPK SFLGFMPLYL AVEIVLGISI LNKCSGAYGI LALFTGHPLD FMQWIAYLWS
VFTLIVFSQG LYLIHKPNLL VFSQICVLYT IDTISTCFFT LWFTTQWFTL EDTANIDGNN
ALQSNPISTG KLTERGIDIS KQSATESYEY TMTILITLVS LIFRFYFNFI LASFVQELLH
HPKYLVDRDD VEQNLKNKPI WKRLWAKSQK GCYKLCKNLL E
