KEL2_YEAST
ID KEL2_YEAST Reviewed; 882 AA.
AC P50090; D6VV18;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=Kelch repeat-containing protein 2;
GN Name=KEL2; OrderedLocusNames=YGR238C; ORFNames=G8585;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8701610;
RX DOI=10.1002/(sici)1097-0061(19960330)12:4<385::aid-yea910>3.0.co;2-g;
RA van der Aart Q.J.M., Kleine K., Steensma H.Y.;
RT "Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1
RT region from the right arm of Saccharomyces cerevisiae chromosome VII.";
RL Yeast 12:385-390(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP CHARACTERIZATION.
RX PubMed=9786949; DOI=10.1083/jcb.143.2.375;
RA Philips J., Herskowitz I.;
RT "Identification of Kel1p, a kelch domain-containing protein involved in
RT cell fusion and morphology in Saccharomyces cerevisiae.";
RL J. Cell Biol. 143:375-389(1998).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-455 AND SER-509, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- SUBUNIT: Interacts with KEL1.
CC -!- INTERACTION:
CC P50090; P38853: KEL1; NbExp=6; IntAct=EBI-9630, EBI-9619;
CC -!- MISCELLANEOUS: Present with 468 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X87941; CAA61189.1; -; Genomic_DNA.
DR EMBL; Z73023; CAA97266.1; -; Genomic_DNA.
DR EMBL; AY693035; AAT93054.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08329.1; -; Genomic_DNA.
DR PIR; S57704; S57704.
DR RefSeq; NP_011754.3; NM_001181367.3.
DR AlphaFoldDB; P50090; -.
DR SMR; P50090; -.
DR BioGRID; 33490; 160.
DR ComplexPortal; CPX-36; Kelch-containing Formin Regulatory Complex.
DR DIP; DIP-4229N; -.
DR IntAct; P50090; 10.
DR MINT; P50090; -.
DR STRING; 4932.YGR238C; -.
DR iPTMnet; P50090; -.
DR MaxQB; P50090; -.
DR PaxDb; P50090; -.
DR PRIDE; P50090; -.
DR EnsemblFungi; YGR238C_mRNA; YGR238C; YGR238C.
DR GeneID; 853153; -.
DR KEGG; sce:YGR238C; -.
DR SGD; S000003470; KEL2.
DR VEuPathDB; FungiDB:YGR238C; -.
DR eggNOG; KOG0379; Eukaryota.
DR GeneTree; ENSGT00940000176597; -.
DR HOGENOM; CLU_005472_0_0_1; -.
DR InParanoid; P50090; -.
DR BioCyc; YEAST:G3O-30916-MON; -.
DR PRO; PR:P50090; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P50090; protein.
DR GO; GO:0051285; C:cell cortex of cell tip; IBA:GO_Central.
DR GO; GO:0005935; C:cellular bud neck; IDA:SGD.
DR GO; GO:0005934; C:cellular bud tip; IDA:SGD.
DR GO; GO:1990615; C:Kelch-containing formin regulatory complex; IDA:SGD.
DR GO; GO:0043332; C:mating projection tip; IDA:SGD.
DR GO; GO:0000747; P:conjugation with cellular fusion; IMP:SGD.
DR GO; GO:0001100; P:negative regulation of exit from mitosis; IMP:SGD.
DR GO; GO:0008360; P:regulation of cell shape; IBA:GO_Central.
DR GO; GO:0032465; P:regulation of cytokinesis; IGI:SGD.
DR GO; GO:0090337; P:regulation of formin-nucleated actin cable assembly; IGI:SGD.
DR GO; GO:0060627; P:regulation of vesicle-mediated transport; IMP:SGD.
DR Gene3D; 2.120.10.80; -; 2.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR SUPFAM; SSF117281; SSF117281; 2.
PE 1: Evidence at protein level;
KW Coiled coil; Kelch repeat; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..882
FT /note="Kelch repeat-containing protein 2"
FT /id="PRO_0000119097"
FT REPEAT 99..143
FT /note="Kelch 1"
FT REPEAT 153..207
FT /note="Kelch 2"
FT REPEAT 213..267
FT /note="Kelch 3"
FT REPEAT 268..317
FT /note="Kelch 4"
FT REPEAT 319..369
FT /note="Kelch 5"
FT REPEAT 371..417
FT /note="Kelch 6"
FT REGION 41..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 480..516
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 550..685
FT /evidence="ECO:0000255"
FT COILED 728..881
FT /evidence="ECO:0000255"
FT COMPBIAS 481..501
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 455
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 509
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
SQ SEQUENCE 882 AA; 99975 MW; 63B9772FF017E6D9 CRC64;
MVPFKLTNKV PTDTGPSLIS AQSVPRPIVF MDNRNNTRIV TPTLPPNQHR GISGASTALP
WSPESKNTGK YIWNRVKLKN SPFPRYRHSS SFIVTNDNRI FVTGGLHDQS VYGDVWQIAA
NADGTSFTSK RIDIDQNTPP PRVGHASTIC GNAYVVFGGD THKLNKNGLL DDDLYLFNIN
SYKWTIPQPI GRRPLGRYGH KISIIASNPM QTKLYLFGGQ VDETYFNDLV VFDLSSFRRP
NSHWEFLEPV GDLPPPLTNH TMVAYDNKLW VFGGETPKTI SNDTYRYDPA QSEWSKVKTT
GEKPPPIQEH ASVVYKHLMC VLGGKDTHNA YSNDVYFLNL LSLKWYKLPR MKEGIPQERS
GHSLTLMKNE KLLIMGGDKT DYASPNIHDL QTSETDQGEG TLLYTLDLSS LNELCPGIMC
ESLHAGESFS NSLSGGFTPS KSTESENQEI INILTPRLPD SKVLSYNDID EGAGSYSSAL
DDKAFERKSD REEKKPQSSK VDSSINKESP GTGIKVSKKN FPVLRGLTVD SEEYGSSSYK
DTSCQKGIPK NLFDDLNLNL QTLRLEAQQK ELETARHISQ LEKEVQRLMV IKEASKDSNF
QTARLKNLEI QKTFLESRIN DLKNLLMVKL SQASKLCDQI TIQNNGLKTC SEHVTIKRDI
IDLENKCDVL KRQNEILVNN MQKITPELHT YLNESSCYLG KLLKSYPTSA RPPSSEKDNQ
IYEKDSLNKI EKVINEMHET VRAKEKLHLE TQKLNDERDS LRANLLDNNN KLDALRKLSD
GSSKSMDLTK KAIHLSQSEL EKYRKNNDDL QKEIDRIKTE QAEQDDKQEQ RGAITHGNFD
AFHRMKINNL KAELYMSKEN RDSLKDELLA LKKKLYTLEQ KK
